Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	243,760	A→G	N73S (AAC→AGC)	lpcA →	D‑sedoheptulose 7‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	243,760	0	A	G	100.0%	130.2 / NA	37	N73S (AAC→AGC)	lpcA	D‑sedoheptulose 7‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/26);  total (11/26)

ACGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAACCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGAAGGCGATGTACTGCTGGGG  >  NC_000913/243625‑243893                                                                                                                                         |                                                                                                                                      aCGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCCTTGCGACGCTATGCACTTTTCCGCAGA‑GTTGACCGGGCGCTACCGTGAAAACCgc                                                                                                                                   >  2:879326/1‑139 (MQ=255)      aTTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCCTTGCGACGCCATGCACTTTTCCGAAGA‑GTTGGCCGGGCGCTACCCTGAAAACCGTCCcgg                                                                                                                              >  2:20659/1‑140 (MQ=255)       ttCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTTCCCTTGCGGCGCCATGCACTTTTCCGGAGA‑GGTGGCCGGGCGCCACCCCGAAAACCGTCCGGGc                                                                                                                             >  2:929480/1‑140 (MQ=255)       ttCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGc                                                                                                                             <  2:316774/140‑1 (MQ=255)        tCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCCTTGCGACGCCATGCACTTTTCCGGAGA‑GGTGAACGGGCGCTACCCCGAAAGCCGGCCCGGCt                                                                                                                            >  2:922013/1‑140 (MQ=255)             gcgcgGCGGTCCTGTTAGCAGACAGCTTTAAAGCGGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTTCCCCTTCGGCGCTATGCACTTTGTCGGAGAGGTGGACCGG‑CGCCACCCTGAAAACCCTCCggggcccccc                                                                                                                       >  2:858917/1‑133 (MQ=255)                 ggcggTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCCTTGCGACGCTATGCACTTTTCCGAAGA‑GGTGACCGGTCGCTACCGGGAAAGCCGTCCGGGCGACCCcgcgg                                                                                                                   >  2:321511/1‑139 (MQ=255)                 ggcggTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCCCTGCGGCGCCATGGACTTTTCCGAAGA‑GGTGACCCGGCGCTACCCGGGAAGCCGTCCCGGCTACCCcgcgg                                                                                                                   >  2:892614/1‑139 (MQ=255)                  gcggTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGAt                                                                                                                  <  2:16091/140‑1 (MQ=255)                      gccTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCt                                                                                                              <  1:309412/139‑1 (MQ=255)                       ccTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTa                                                                                                             <  1:892614/140‑1 (MQ=255)                               cAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCCTTTCGGCGCCATGCACTTTTCCGAAGA‑GGTGACCGGGCGCTACCCGGAAAACCGGCCCGGCTACCCCGCGGGTGCTAttttttac                                                                                                     >  2:476542/1‑135 (MQ=255)                               cAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGAc                                                                                                     <  2:251991/140‑1 (MQ=255)                                  aCAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTTCCCTTGCGGCGCTATGCACTTTTCCGGAGA‑GTTGACCGGGCGCTACCCCGGAAACCGGCCGGGGTACCCCGCGATTGCTATTTTTTACGtc                                                                                                  >  2:309412/1‑139 (MQ=255)                                   cAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTa                                                                                                 <  1:922013/140‑1 (MQ=255)                                             gCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTc                                                                                       <  1:307350/140‑1 (MQ=255)                                                 gTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGc                                                                                   <  1:858917/140‑1 (MQ=255)                                                  tGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCg                                                                                  <  1:613129/140‑1 (MQ=255)                                                                    cGGCAACGGCGGTTCCCCTTGCGACGCTATGCACTTTTCCGGAGA‑GTTGACCGGGCGCTACCGTGAAAGCCGTCCGGGCTACCCCGCGGTTGCTATTTCTGACGTTAGTCATATTTTCTGCGTCGGGAATGATTTTGGtt                                                                >  2:886128/1‑140 (MQ=255)                                                                         aCGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAAt                                                           <  1:314118/140‑1 (MQ=255)                                                                                      ttGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGCCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTccc                                              >  2:959692/1‑140 (MQ=255)                                                                                       tGCGGCGCTATGCACTTTGGCGGAGA‑GTTGGCCCGTCGCTACCGTGAAAGCCGGCCGGGCTTCCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCg                                             <  2:729270/140‑1 (MQ=255)                                                                                       tGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCg                                             <  1:886128/140‑1 (MQ=255)                                                                                         cGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCt                                           >  2:1004746/1‑140 (MQ=255)                                                                                           aCGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCg                                             >  1:303994/1‑136 (MQ=255)                                                                                           aCGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCg                                             <  2:303994/136‑1 (MQ=255)                                                                                            cGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACg                                        <  1:1085028/140‑1 (MQ=255)                                                                                            cGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACg                                        >  2:253441/1‑140 (MQ=255)                                                                                                 tGCACTTTGCCGAAGA‑GTTTACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGaa                                   >  1:356308/1‑140 (MQ=255)                                                                                                 tGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGaa                                   >  1:1027033/1‑140 (MQ=255)                                                                                                 tGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGaa                                   >  1:839728/1‑140 (MQ=255)                                                                                                  gCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAg                                  <  1:573024/140‑1 (MQ=255)                                                                                                   cACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGc                                 >  2:328625/1‑140 (MQ=255)                                                                                                    aCTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCg                                <  1:20659/140‑1 (MQ=255)                                                                                                        tGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAg                            >  2:656162/1‑140 (MQ=255)                                                                                                             aaGA‑GTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTcgc                       >  2:500936/1‑140 (MQ=255)                                                                                                              aga‑gTTTACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTcgcg                      <  2:356308/140‑1 (MQ=255)                                                                                                               ga‑gTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGa                     <  2:600688/140‑1 (MQ=255)                                                                                                               ga‑gTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGa                     <  2:861006/140‑1 (MQ=255)                                                                                                               ga‑gTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGa                     <  1:253441/140‑1 (MQ=255)                                                                                                               ga‑gTTGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGa                     <  1:960590/140‑1 (MQ=255)                                                                                                                   ttGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGAAgg                  <  2:619708/140‑1 (MQ=255)                                                                                                                   ttGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGAAgg                  >  1:893446/1‑140 (MQ=255)                                                                                                                   ttGACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGAAgg                  <  1:266334/140‑1 (MQ=255)                                                                                                                     gACCGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGAAGGCg                <  2:893446/140‑1 (MQ=255)                                                                                                                        cGGTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGAAGGCGATg             <  2:1079290/140‑1 (MQ=255)                                                                                                                          gTCGCTACCGTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGAAGGCGATGTa           <  1:63421/140‑1 (MQ=255)                                                                                                                                   gTGAAAGCCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGAAGGCGATGTACTGCTgggg  <  1:929480/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      ACGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGA‑GTTGACCGGTCGCTACCGTGAAAACCGTCCGGGCTACCCGGCGATTGCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATTTTCTCCCGCTACGTTGAAGCGGTAGGTCGCGAAGGCGATGTACTGCTGGGG  >  NC_000913/243625‑243893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑