| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,883,287 | A→G | C284C (TGT→TGC) | casA ← | type I‑E CRISPR system Cascade subunit CasA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,883,287 | 0 | A | G | 97.9% | 166.3 / ‑4.5 | 48 | C284C (TGT→TGC) | casA | type I‑E CRISPR system Cascade subunit CasA |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (27/20); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTC > NC_000913/2883151‑2883421 | ccgccgCCACGAGAGTACCAGTTGCATGTTGAAGAATGTTAGCTACCACAAATCGGCTGATTTGTGTCCATGAAGGTACGGAGGTGGTGAAAGCAAGAAATTTTCCCTCAACCTCCCCTTCCTTGACTGTTACCAGACaa < 2:174810/140‑1 (MQ=255) ccgccACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAggg > 1:636273/1‑140 (MQ=255) cACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAAt < 2:270062/140‑1 (MQ=255) attttcattttGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGcc < 1:897529/140‑1 (MQ=255) attttcattttGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGcc < 1:437467/140‑1 (MQ=255) attttcattttGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGcc < 1:642991/140‑1 (MQ=255) tttGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCa < 2:589483/140‑1 (MQ=255) ttGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCAt > 2:1082817/1‑140 (MQ=255) tGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCAtt < 2:822211/140‑1 (MQ=255) tGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCAtt > 1:172654/1‑140 (MQ=255) tGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCAtt < 2:717831/140‑1 (MQ=255) tCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTa > 2:995433/1‑140 (MQ=255) tCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGt > 2:147820/1‑140 (MQ=255) cTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTa > 1:722212/1‑140 (MQ=255) cacaACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAAtt > 1:476250/1‑140 (MQ=255) cacaACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAAtt > 2:804575/1‑140 (MQ=255) cTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTcc > 2:348467/1‑140 (MQ=255) cTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTcc > 2:935043/1‑140 (MQ=255) cGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCtt > 2:660595/1‑140 (MQ=255) gCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGTATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTaa > 1:244425/1‑140 (MQ=255) gCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTaa > 1:514210/1‑140 (MQ=255) gCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTaa > 1:639371/1‑140 (MQ=255) cTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAg > 1:193884/1‑140 (MQ=255) cTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAg < 2:15551/140‑1 (MQ=255) atgatgGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATaa > 1:578401/1‑140 (MQ=255) tgatgGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAAc > 1:1029370/1‑140 (MQ=255) gatgGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAAc < 1:1056124/130‑1 (MQ=255) gatgGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAAc > 2:1056124/1‑130 (MQ=255) tgGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCa < 1:1091314/140‑1 (MQ=255) ggtggtGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCttt > 2:721197/1‑140 (MQ=255) gtggtgAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTc < 1:1082817/140‑1 (MQ=255) gtggtgAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTc < 2:193884/140‑1 (MQ=255) gtggtgAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTc < 2:636273/140‑1 (MQ=255) gtgAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTg > 2:1030749/1‑140 (MQ=255) cAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGTGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAg > 1:877536/1‑140 (MQ=255) aGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGTGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCa < 2:877536/140‑1 (MQ=255) gAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCaa > 2:630177/1‑140 (MQ=255) gAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAAAGCAAATTGCTTTCCTGTCCACAGCaa < 1:804575/140‑1 (MQ=255) cTCAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTAc < 2:1029370/140‑1 (MQ=255) cAACCTCCCCTTTCTTGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCa < 1:348467/140‑1 (MQ=255) tGACTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCg < 1:147820/140‑1 (MQ=255) cTGTTACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCAt < 1:167492/140‑1 (MQ=255) ttACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGa < 2:33621/131‑1 (MQ=255) ttACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGa > 1:33621/1‑131 (MQ=255) ttACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAAt > 2:248128/1‑140 (MQ=255) tACCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAAtt > 1:651636/1‑140 (MQ=255) aCCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTc > 1:339820/1‑140 (MQ=255) aCCAGGCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTc > 2:706583/1‑140 (MQ=255) | CCGCCGCCACGCGATTTCCATTTTCATTTTGAATAATCTTATCTACCACAACTCGGCTGATTTGTGTCCATGATGGTGCGGAGGTGGTGAAAGCAAGAAATTTTTCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTC > NC_000913/2883151‑2883421 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |