Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,906,780	C→T	A388T (GCT→ACT)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,906,780	0	C	T	100.0%	169.9 / NA	48	A388T (GCT→ACT)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/20);  total (28/20)

TATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAAT  >  NC_000913/2906644‑2906916                                                                                                                                         |                                                                                                                                         tATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCagtagt                                                                                                                                       <  2:98363/140‑1 (MQ=255)     ccTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCa                                                                                                                                   <  2:270702/140‑1 (MQ=255)        ggCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACa                                                                                                                                >  1:145175/1‑140 (MQ=255)        ggCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACa                                                                                                                                >  1:765572/1‑140 (MQ=255)        ggCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACa                                                                                                                                >  1:319857/1‑140 (MQ=255)          ccTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGc                                                                                                                              <  1:44768/140‑1 (MQ=255)          ccTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGc                                                                                                                              >  2:44768/1‑140 (MQ=255)          ccTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGc                                                                                                                              <  2:319857/140‑1 (MQ=255)                aTCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTc                                                                                                                        <  2:765572/140‑1 (MQ=255)                  ctctTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAg                                                                                                                      <  1:1068948/140‑1 (MQ=255)                     tttACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCg                                                                                                                    >  1:375715/1‑139 (MQ=255)                     tttACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCg                                                                                                                    <  2:375715/139‑1 (MQ=255)                          gACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTa                                                                                                              >  1:594935/1‑140 (MQ=255)                               ttGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTc                                                                                                         >  1:185107/1‑140 (MQ=255)                                       tGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGttt                                                                                                 >  1:611326/1‑140 (MQ=255)                                       tGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGttt                                                                                                 >  2:1012884/1‑140 (MQ=255)                                         cTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCg                                                                                               >  2:377605/1‑140 (MQ=255)                                          tttttCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGc                                                                                              <  2:145175/140‑1 (MQ=255)                                           ttttCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGcc                                                                                             <  2:326385/140‑1 (MQ=255)                                                 cccAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAg                                                                                           >  1:770790/1‑136 (MQ=255)                                                 cccAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAg                                                                                           <  2:770790/136‑1 (MQ=255)                                                    agagCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTg                                                                                    <  2:611326/140‑1 (MQ=255)                                                      agCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTgag                                                                                  >  1:910307/1‑140 (MQ=255)                                                                aTACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGc                                                                        >  1:67656/1‑140 (MQ=255)                                                                     aaTCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTgaga                                                                                 >  2:983679/1‑126 (MQ=255)                                                                     aaTCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTgaga                                                                                 <  1:983679/126‑1 (MQ=255)                                                                              gTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCAt                                                          >  2:319201/1‑140 (MQ=255)                                                                                tGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCt                                                        >  2:411221/1‑140 (MQ=255)                                                                                tGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCt                                                        >  2:797199/1‑140 (MQ=255)                                                                                    tttAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCg                                                    >  1:244206/1‑140 (MQ=255)                                                                                      tAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGcc                                                  <  1:797199/140‑1 (MQ=255)                                                                                      tAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGcc                                                  <  1:430758/140‑1 (MQ=255)                                                                                         cAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATc                                               >  1:863758/1‑140 (MQ=255)                                                                                            cacGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTg                                            >  1:487899/1‑140 (MQ=255)                                                                                              cGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGAt                                          <  1:377605/140‑1 (MQ=255)                                                                                              cGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGAt                                          <  1:411221/140‑1 (MQ=255)                                                                                                 tCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGc                                       >  2:332798/1‑140 (MQ=255)                                                                                                   aGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCg                                                    <  1:537306/125‑1 (MQ=255)                                                                                                   aGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCg                                                    >  2:537306/1‑125 (MQ=255)                                                                                                          cTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGt                              >  1:792293/1‑140 (MQ=255)                                                                                                          cTCATAGAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGt                              >  2:675651/1‑140 (MQ=255)                                                                                                                gAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGt                        <  1:332798/140‑1 (MQ=255)                                                                                                                gAACCAGTTTTGATCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGt                        <  2:863758/140‑1 (MQ=255)                                                                                                                            aTCTGGCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGAt            <  2:67656/140‑1 (MQ=255)                                                                                                                                 gCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGt       >  2:718208/1‑140 (MQ=255)                                                                                                                                 gCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGt       >  2:862839/1‑140 (MQ=255)                                                                                                                                 gCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGt       >  2:389398/1‑140 (MQ=255)                                                                                                                                 gCCTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTAAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGt       >  2:575644/1‑140 (MQ=255)                                                                                                                                   cTGCAGTAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTg     <  2:910307/140‑1 (MQ=255)                                                                                                                                      cagtagTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAAt  <  2:359849/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGATGGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAAT  >  NC_000913/2906644‑2906916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑