Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,916	G→A	P59L (CCG→CTG)	mazF ←	endoribonuclease toxin MazF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,916	0	G	A	100.0%	212.7 / NA	59	P59L (CCG→CTG)	mazF	endoribonuclease toxin MazF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/31);  total (28/31)

GTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAC  >  NC_000913/2910791‑2911049                                                                                                                              |                                                                                                                                      gTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGAt                                                                                                                         >  1:105017/1‑140 (MQ=255)  ttGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGAtt                                                                                                                        <  2:179521/140‑1 (MQ=255)  ttGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGAtt                                                                                                                        <  2:532172/140‑1 (MQ=255)    gTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGc                                                                                                                      <  1:64236/140‑1 (MQ=255)     tAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCg                                                                                                                     >  1:1047120/1‑140 (MQ=255)     tAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCg                                                                                                                     <  2:105017/140‑1 (MQ=255)     tAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCg                                                                                                                     <  2:1047120/140‑1 (MQ=255)     tAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCg                                                                                                                     <  2:385233/140‑1 (MQ=255)     tAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCg                                                                                                                     <  1:784554/140‑1 (MQ=255)     tAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCg                                                                                                                     <  1:461175/140‑1 (MQ=255)      aaTTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGt                                                                                                                    >  2:218255/1‑140 (MQ=255)       aTTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGtt                                                                                                                   >  2:655266/1‑140 (MQ=255)        ttCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTg                                                                                                                  <  2:80422/140‑1 (MQ=255)           ctctGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTAc                                                                                                               <  2:348226/140‑1 (MQ=255)             ctGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACaa                                                                                                             <  1:534246/140‑1 (MQ=255)               ggggCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAgg                                                                                                           <  2:1039495/140‑1 (MQ=255)                   cAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACa                                                                                                              >  1:606594/1‑133 (MQ=255)                   cAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACa                                                                                                              <  2:606594/133‑1 (MQ=255)                     aCTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAacaca                                                                                                     <  1:130249/140‑1 (MQ=255)                         ttCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGa                                                                                                 <  1:203072/140‑1 (MQ=255)                          tCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGac                                                                                                >  1:654456/1‑140 (MQ=255)                             tttCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGAcaca                                                                                             >  2:682254/1‑140 (MQ=255)                                 ttcGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATAcc                                                                                         >  2:222252/1‑140 (MQ=255)                                                  ccGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTttgttg                                                                               <  2:732357/133‑1 (MQ=255)                                                  ccGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTttgttg                                                                               >  1:732357/1‑133 (MQ=255)                                                     ccAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGa                                                                     >  1:18188/1‑140 (MQ=255)                                                       aGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGaaa                                                                   <  1:472261/140‑1 (MQ=255)                                                       aGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGaaa                                                                   <  1:203687/140‑1 (MQ=255)                                                                   ttACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGaca                                                       >  2:1084730/1‑140 (MQ=255)                                                                    tACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGacaa                                                      <  1:171/140‑1 (MQ=255)                                                                          aTCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTg                                                >  1:180203/1‑140 (MQ=255)                                                                              gCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCCGGACg                                            <  2:654456/140‑1 (MQ=255)                                                                               cTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGa                                           >  2:86025/1‑140 (MQ=255)                                                                                    gCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTcc                                      >  2:914716/1‑140 (MQ=255)                                                                                    gCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTcc                                      >  1:2548/1‑140 (MQ=255)                                                                                       aCGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGc                                   >  2:265635/1‑140 (MQ=255)                                                                                       aCGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGc                                   >  2:142636/1‑140 (MQ=255)                                                                                       aCGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGc                                   <  1:222252/140‑1 (MQ=255)                                                                                       aCGCCACCACATTCCTGAGCTAATAAAGCAACTTCGAACAGAAATCCTTTTGATTGCGTTGTACAAGGAACACACAGCCACATACCTGGTTTGTTGATGAACATGAAAGGACTCAGGACAACAACTGGACGATGTCCatc                                   >  2:981925/1‑138 (MQ=255)                                                                                        cGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCt                                  >  1:1047850/1‑140 (MQ=255)                                                                                        cGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCt                                  <  1:265635/140‑1 (MQ=255)                                                                                         gCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCtt                                 >  2:626304/1‑140 (MQ=255)                                                                                         gCCATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCtt                                 >  1:487309/1‑140 (MQ=255)                                                                                          ccATCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTg                                <  1:142636/140‑1 (MQ=255)                                                                                             tCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                             <  1:850639/140‑1 (MQ=255)                                                                                             tCACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTc                             >  1:801528/1‑140 (MQ=255)                                                                                              cACGTTCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCg                            >  2:136633/1‑140 (MQ=255)                                                                                                   tCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTAcc                       <  2:926551/140‑1 (MQ=255)                                                                                                   tCCTGACCGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTAcc                       >  2:594402/1‑140 (MQ=255)                                                                                                         ccGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGt                 <  1:433443/140‑1 (MQ=255)                                                                                                          cGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTc                >  1:957622/1‑140 (MQ=255)                                                                                                          cGGATAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTc                <  1:655266/140‑1 (MQ=255)                                                                                                             aTAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCggg             >  1:511343/1‑140 (MQ=255)                                                                                                             aTAAAACAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCggg             >  2:570489/1‑140 (MQ=255)                                                                                                               aaaaCAACTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATTAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTc           <  1:864903/140‑1 (MQ=255)                                                                                                                    aacTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAAt      <  1:136633/140‑1 (MQ=255)                                                                                                                    aacTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCGTTTGTCGGGTCAAAAt      <  2:957622/140‑1 (MQ=255)                                                                                                                      cTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCa    >  2:820391/1‑140 (MQ=255)                                                                                                                      cTTCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCa    >  1:243375/1‑140 (MQ=255)                                                                                                                        tCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGCCTCAGGACAACAGGTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAc  <  2:768711/140‑1 (MQ=255)                                                                                                                        tCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAc  <  1:570489/140‑1 (MQ=255)                                                                                                                        tCGAACAGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAc  <  2:511343/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                      GTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAC  >  NC_000913/2910791‑2911049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑