Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,920,095	T→C	T218A (ACT→GCT)	gudX ←	glucarate dehydratase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,920,095	0	T	C	100.0%	211.3 / NA	59	T218A (ACT→GCT)	gudX	glucarate dehydratase‑related protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/28);  total (31/28)

CGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATA  >  NC_000913/2919960‑2920227                                                                                                                                        |                                                                                                                                     cgcATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCg                                                                                                                                  >  1:136636/1‑140 (MQ=255)         tCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTGCTTCAATGCACGAACAGCGTCGATTTCTTg                                                                                                                          <  2:192144/140‑1 (MQ=255)           ttCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCt                                                                                                                        <  1:455335/140‑1 (MQ=255)             cGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCg                                                                                                                      <  2:177105/140‑1 (MQ=255)             cGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCg                                                                                                                      <  2:36726/140‑1 (MQ=255)                  tAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCg                                                                                                                      >  1:310756/1‑135 (MQ=255)                  tAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCg                                                                                                                      <  2:310756/135‑1 (MQ=255)                   aGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGt                                                                                                                >  2:860037/1‑140 (MQ=255)                     gTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCt                                                                                                                        <  2:1065429/130‑1 (MQ=255)                     gTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCt                                                                                                                        >  1:1065429/1‑130 (MQ=255)                          aaCATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACAcgc                                                                                                         >  2:242340/1‑140 (MQ=255)                            catcatTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGcc                                                                                                                    <  1:897873/127‑1 (MQ=255)                            catcatTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGcc                                                                                                                    >  2:897873/1‑127 (MQ=255)                              tcatTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACAcgcc                                                                                                        >  1:196868/1‑137 (MQ=255)                              tcatTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACAcgcc                                                                                                        <  2:196868/137‑1 (MQ=255)                              tcatTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACgccgcc                                                                                                     >  2:102253/1‑140 (MQ=255)                               catTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGccc                                                                                                    >  1:441106/1‑140 (MQ=255)                                    aGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAg                                                                                               >  2:977558/1‑140 (MQ=255)                                           tGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGaaa                                                                                        >  2:27641/1‑140 (MQ=255)                                           tGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGaaa                                                                                        >  2:422538/1‑140 (MQ=255)                                                   gAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTaaa                                                                                <  1:860037/140‑1 (MQ=255)                                                       tGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCg                                                                            >  1:434783/1‑140 (MQ=255)                                                          cTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAg                                                                         <  1:422538/140‑1 (MQ=255)                                                            tcatcaAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCg                                                                       >  2:50914/1‑140 (MQ=255)                                                                       ccATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAgg                                                            >  1:228049/1‑140 (MQ=255)                                                                       ccATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCG‑TCCTGTGAGGc                                                           >  1:848020/1‑140 (MQ=255)                                                                          tGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCtt                                                         >  2:1046941/1‑140 (MQ=255)                                                                            cACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTcc                                                       <  1:578118/140‑1 (MQ=255)                                                                            cACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTcc                                                       >  1:31534/1‑140 (MQ=255)                                                                              ccGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCATTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGc                                                     <  2:238961/140‑1 (MQ=255)                                                                               cGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGcc                                                    <  1:102253/140‑1 (MQ=255)                                                                               cGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGcc                                                    <  2:228049/140‑1 (MQ=255)                                                                               cGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGcc                                                    <  2:434783/140‑1 (MQ=255)                                                                               cGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGcc                                                    >  1:265036/1‑140 (MQ=255)                                                                                 ttGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAgg                                                            <  1:925189/130‑1 (MQ=255)                                                                                 ttGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAgg                                                            >  2:925189/1‑130 (MQ=255)                                                                                 ttGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCG‑TCCTGTGAGGCTTCCGCCAGa                                                 <  2:848020/140‑1 (MQ=255)                                                                                      aTCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGca                                             >  2:468861/1‑140 (MQ=255)                                                                                       tCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGcac                                            <  1:50914/140‑1 (MQ=255)                                                                                        cAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGcaca                                           >  2:473972/1‑140 (MQ=255)                                                                                         aaCGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACTAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACaa                                          >  2:396962/1‑140 (MQ=255)                                                                                         aaCGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACaa                                          >  2:233604/1‑140 (MQ=255)                                                                                              tAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCt                                     <  1:473972/140‑1 (MQ=255)                                                                                                aTCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTc                                   >  1:486496/1‑140 (MQ=255)                                                                                                     cgcATCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGt                              >  1:745506/1‑140 (MQ=255)                                                                                                        aTCCGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCa                           <  2:441106/140‑1 (MQ=255)                                                                                                          ccGGGAAGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCAtt                         >  2:702655/1‑140 (MQ=255)                                                                                                                aGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGt                              >  2:360778/1‑129 (MQ=255)                                                                                                                aGCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGt                              <  1:360778/129‑1 (MQ=255)                                                                                                                 gCGTTTCTTCAATGCACTAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCt                  <  1:396962/140‑1 (MQ=255)                                                                                                                 gCGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCt                  <  1:233604/140‑1 (MQ=255)                                                                                                                  cGTTTCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTg                 >  2:1041246/1‑140 (MQ=255)                                                                                                                    tttCTTCAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGAt               >  1:1067966/1‑140 (MQ=255)                                                                                                                       cttcAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGcc            <  1:649507/140‑1 (MQ=255)                                                                                                                        ttcAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCg           <  1:468861/140‑1 (MQ=255)                                                                                                                        ttcAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCg           <  1:242340/140‑1 (MQ=255)                                                                                                                          cAATGCACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCa         <  1:1046941/140‑1 (MQ=255)                                                                                                                               cACGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTGAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGGACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGa    >  2:752421/1‑140 (MQ=255)                                                                                                                                 cGAACAGCGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATa  <  1:977558/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     CGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATA  >  NC_000913/2919960‑2920227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑