Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,942,553	T→C	L5L (TTA→TTG)	gcvA ←	DNA‑binding transcriptional dual regulator GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,942,553	0	T	C	100.0%	213.0 / NA	59	L5L (TTA→TTG)	gcvA	DNA‑binding transcriptional dual regulator GcvA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/35);  total (24/35)

GCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAG  >  NC_000913/2942420‑2942688                                                                                                                                      |                                                                                                                                        gCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGt                                                                                                                                   >  1:643980/1‑140 (MQ=255)  ccccAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGtt                                                                                                                                  <  1:469005/140‑1 (MQ=255)      aaaaaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCaa                                                                                                                                       >  2:425055/1‑131 (MQ=255)      aaaaaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGa                                                                                                                              <  1:266424/140‑1 (MQ=255)      aaaaaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGa                                                                                                                              >  1:11807/1‑140 (MQ=255)         aaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACAt                                                                                                                           <  2:252945/140‑1 (MQ=255)                cAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTAtt                                                                                                                    <  2:161575/140‑1 (MQ=255)                      aCTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACtt                                                                                                              >  1:497676/1‑140 (MQ=255)                        ttgatttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACAt                                                                                                                           <  2:704561/125‑1 (MQ=255)                        ttgatttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACAt                                                                                                                           >  1:704561/1‑125 (MQ=255)                           atttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACtt                                                                                                              <  2:403698/135‑1 (MQ=255)                           atttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACtt                                                                                                              >  1:403698/1‑135 (MQ=255)                            tttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGt                                                                                                        <  2:746587/140‑1 (MQ=255)                             ttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGtt                                                                                                       >  2:169052/1‑140 (MQ=255)                                atgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTaaa                                                                                                    >  1:532095/1‑140 (MQ=255)                                  gaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATg                                                                                                  >  2:391040/1‑140 (MQ=255)                                     ttACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAAt                                                                                               >  1:86039/1‑140 (MQ=255)                                     ttACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAAt                                                                                               >  2:191729/1‑140 (MQ=255)                                      tACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAAtt                                                                                              >  1:930371/1‑140 (MQ=255)                                       aCTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAAttt                                                                                             <  2:11807/140‑1 (MQ=255)                                       aCTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAAttt                                                                                             <  1:169052/140‑1 (MQ=255)                                        cTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGNNAAANGAATTTa                                                                                            <  2:389503/140‑1 (MQ=255)                                          gCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAAc                                                                                          >  1:1018183/1‑140 (MQ=255)                                            ggCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACaa                                                                                        <  1:577406/140‑1 (MQ=255)                                            ggCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACaa                                                                                        <  2:497676/140‑1 (MQ=255)                                             gCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAAt                                                                                       <  2:220045/140‑1 (MQ=255)                                             gCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAAt                                                                                       >  1:113465/1‑140 (MQ=255)                                                tGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAg                                                                                    >  2:570423/1‑140 (MQ=255)                                                 gggTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGa                                                                                   <  1:985048/140‑1 (MQ=255)                                                 gggTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGa                                                                                   <  2:113465/140‑1 (MQ=255)                                                  ggTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGAt                                                                                  >  2:135676/1‑140 (MQ=255)                                                    tCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATca                                                                                <  2:95196/140‑1 (MQ=255)                                                    tCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATca                                                                                <  1:570423/140‑1 (MQ=255)                                                     cacaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcac                                                                               <  1:1032163/140‑1 (MQ=255)                                                     cacaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcac                                                                               <  2:86039/140‑1 (MQ=255)                                                     cacaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcac                                                                               <  1:191729/140‑1 (MQ=255)                                                     cacaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcac                                                                               <  1:135676/140‑1 (MQ=255)                                                      acaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcaca                                                                              <  2:643980/140‑1 (MQ=255)                                                      acaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcaca                                                                              <  2:608026/140‑1 (MQ=255)                                                      acaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcaca                                                                              <  2:532095/140‑1 (MQ=255)                                                       caAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGa                                                                                   >  2:279142/1‑134 (MQ=255)                                                       caAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGa                                                                                   <  1:279142/134‑1 (MQ=255)                                                       caAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCacac                                                                             >  1:50786/1‑140 (MQ=255)                                                               tcttctGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAAc                                                                     <  2:930371/140‑1 (MQ=255)                                                                  tctGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTa                                                                  >  2:367913/1‑140 (MQ=255)                                                                      ctgcGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAg                                                              <  2:664697/140‑1 (MQ=255)                                                                      ctgcGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAg                                                              <  1:367913/140‑1 (MQ=255)                                                                      ctgcGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAg                                                              >  2:849737/1‑140 (MQ=255)                                                                           cgAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGttttt                                                         >  1:103782/1‑140 (MQ=255)                                                                           cgAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGttttt                                                         >  1:822988/1‑140 (MQ=255)                                                                                     ttAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAg                                               <  2:822988/140‑1 (MQ=255)                                                                                     ttAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAg                                               <  2:50786/140‑1 (MQ=255)                                                                                     ttAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAg                                               <  1:83751/140‑1 (MQ=255)                                                                                     ttAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAg                                               <  1:849737/140‑1 (MQ=255)                                                                                     ttAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAg                                               <  2:103782/140‑1 (MQ=255)                                                                                        aaTGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCa                                            >  2:854370/1‑140 (MQ=255)                                                                                               tgctgcGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGcc                                             >  1:133678/1‑132 (MQ=255)                                                                                               tgctgcGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGcc                                             <  2:133678/132‑1 (MQ=255)                                                                                                   gcGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGCAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGt                                 <  1:391040/140‑1 (MQ=255)                                                                                                                                  gtggcAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAg  >  1:1062893/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        GCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAG  >  NC_000913/2942420‑2942688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑