Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,978,049	A→G	V284A (GTG→GCG)	lysA ←	diaminopimelate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,978,049	0	A	G	100.0%	256.7 / NA	77	V284A (GTG→GCG)	lysA	diaminopimelate decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/39);  total (38/39)

GTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAAC  >  NC_000913/2977915‑2978185                                                                                                                                       |                                                                                                                                         gtTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGa                                                                                                                                     <  1:1024646/140‑1 (MQ=255)    ccAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGta                                                                                                                                  <  1:458399/140‑1 (MQ=255)           aCGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATa                                                                                                                           <  2:936455/140‑1 (MQ=255)                 aTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCt                                                                                                                                       <  1:956769/122‑1 (MQ=255)                 aTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCt                                                                                                                                       >  2:956769/1‑122 (MQ=255)                 aTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAg                                                                                                                     <  1:436429/140‑1 (MQ=255)                    aGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACt                                                                                                                  >  2:529543/1‑140 (MQ=255)                       tcccAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTgcg                                                                                                               <  2:1013177/138‑1 (MQ=255)                       tGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTgcg                                                                                                               <  1:953851/140‑1 (MQ=255)                           aGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTAc                                                                                                           <  1:196519/140‑1 (MQ=255)                                   gATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGc                                                                                                   >  2:181368/1‑140 (MQ=255)                                    atatGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCg                                                                                                  >  1:44772/1‑140 (MQ=255)                                         gtggtAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCg                                                                                             >  1:613632/1‑140 (MQ=255)                                              aaCTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCa                                                                                        >  2:24250/1‑140 (MQ=255)                                              aaCTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGGAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCa                                                                                        >  2:1033461/1‑140 (MQ=255)                                               aCTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCaa                                                                                       >  1:733200/1‑140 (MQ=255)                                                   ccGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTc                                                                                   >  2:57450/1‑140 (MQ=255)                                                    cGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCa                                                                                        >  2:594678/1‑134 (MQ=255)                                                    cGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCa                                                                                        <  1:594678/134‑1 (MQ=255)                                                    cGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTcc                                                                                  >  1:961141/1‑140 (MQ=255)                                                    cGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTcc                                                                                  >  1:236357/1‑140 (MQ=255)                                                      tACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTTACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAg                                                                                >  2:923950/1‑140 (MQ=255)                                                        cATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGtt                                                                              >  1:1089120/1‑140 (MQ=255)                                                           tGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCa                                                                                 >  2:311817/1‑134 (MQ=255)                                                           tGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCa                                                                                 <  1:311817/134‑1 (MQ=255)                                                           tGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTca                                                                           <  2:607311/140‑1 (MQ=255)                                                           tGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTca                                                                           <  2:236357/140‑1 (MQ=255)                                                           tGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTca                                                                           >  2:300714/1‑140 (MQ=255)                                                             ccGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTcc                                                                                  >  2:303455/1‑131 (MQ=255)                                                             ccGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTcc                                                                                  <  1:303455/131‑1 (MQ=255)                                                             ccGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTcaca                                                                         >  2:210974/1‑140 (MQ=255)                                                             ccGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTcaca                                                                         >  1:55137/1‑140 (MQ=255)                                                             ccGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTcaca                                                                         >  1:107646/1‑140 (MQ=255)                                                               gggCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAgg                                                                       <  2:504697/140‑1 (MQ=255)                                                               gggCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAgg                                                                       <  1:24250/140‑1 (MQ=255)                                                               gggCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAgg                                                                       <  1:923950/140‑1 (MQ=255)                                                                ggCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAggg                                                                      >  2:787429/1‑140 (MQ=255)                                                                  cgcATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGGAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTg                                                                    >  2:529222/1‑140 (MQ=255)                                                                              ttGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATggcg                                                        >  1:1036029/1‑140 (MQ=255)                                                                              ttGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATggcg                                                        >  1:863890/1‑140 (MQ=255)                                                                               tGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATggcgg                                                       >  1:690126/1‑140 (MQ=255)                                                                                 aaCCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATggcgggc                                                     >  2:825572/1‑140 (MQ=255)                                                                                      ggCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGAttt                                                >  1:49483/1‑140 (MQ=255)                                                                                      ggCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGAttt                                                >  1:532346/1‑140 (MQ=255)                                                                                      ggCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGAttt                                                >  1:690808/1‑140 (MQ=255)                                                                                         aTCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCt                                             <  1:18257/140‑1 (MQ=255)                                                                                           cAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTcc                                           >  1:836040/1‑139 (MQ=255)                                                                                           cAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCa                                           >  1:133241/1‑140 (MQ=255)                                                                                             aCCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCAcg                                         <  1:181368/140‑1 (MQ=255)                                                                                               cAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCAcgcg                                       <  1:825572/140‑1 (MQ=255)                                                                                               cAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCAcgcg                                       <  1:787429/140‑1 (MQ=255)                                                                                               cAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCAcgcg                                       <  2:690126/140‑1 (MQ=255)                                                                                                 gCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCAcgcgcg                                     <  2:418026/140‑1 (MQ=255)                                                                                                  cacaAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCgg                                    >  1:845707/1‑140 (MQ=255)                                                                                                   acaAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGc                                   <  2:1036029/140‑1 (MQ=255)                                                                                                   acaAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGc                                   <  2:863890/140‑1 (MQ=255)                                                                                                    caAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCa                                  <  1:626214/140‑1 (MQ=255)                                                                                                    caAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCa                                  <  1:57450/140‑1 (MQ=255)                                                                                                       aGTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTc                               >  1:201840/1‑140 (MQ=255)                                                                                                        gTGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGGAATTAATACGCCAGACTGCGCTACCAGGGAGCGACCCGGCTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGTTCACGCGCGGCATTcc                              >  2:979746/1‑140 (MQ=255)                                                                                                         tGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCca                             <  2:613632/140‑1 (MQ=255)                                                                                                         tGGCGGCTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCca                             <  2:845707/140‑1 (MQ=255)                                                                                                           gcggcTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCcaca                           <  2:733200/140‑1 (MQ=255)                                                                                                           gcggcTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCcaca                           <  1:996668/140‑1 (MQ=255)                                                                                                           gcggcTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCcaca                           <  2:55137/140‑1 (MQ=255)                                                                                                           gcggcTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCcaca                           <  2:1089120/140‑1 (MQ=255)                                                                                                           gcggcTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGCTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCcaca                           <  2:961141/140‑1 (MQ=255)                                                                                                            cggcTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAg                          >  1:737532/1‑140 (MQ=255)                                                                                                             ggcTCCCCATTTGTTTGACGCTCCGTGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGa                         <  2:944073/140‑1 (MQ=255)                                                                                                             ggcTCCCCATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGa                         <  1:346311/140‑1 (MQ=255)                                                                                                                 ccccATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCcaca                           >  1:914467/1‑134 (MQ=255)                                                                                                                 ccccATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCcaca                           <  2:914467/134‑1 (MQ=255)                                                                                                                 ccccATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAc                        <  2:638052/137‑1 (MQ=255)                                                                                                                 ccccATTTGTTTGACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGAc                        >  1:638052/1‑137 (MQ=255)                                                                                                                          tttgACGCTCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGtt            <  1:210974/140‑1 (MQ=255)                                                                                                                                  tCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGtt            <  2:691867/132‑1 (MQ=255)                                                                                                                                  tCCGCGCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGtt            >  1:691867/1‑132 (MQ=255)                                                                                                                                    cgcgCCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAAc  <  2:737532/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAACCCGGCATCAACCAGCACAAAGTGGCGGCTCCCCATTTGTTTGACGCTCCGCACCTGAGTAATTAATACGCCAGACTGCGCTACCAGGAAGCGACCCGGTTCAATTTCCAGTTTCACAGGGTGGCCCAAATGGCGGGCGATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAAC  >  NC_000913/2977915‑2978185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑