Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,978,378	T→C	A174A (GCA→GCG)	lysA ←	diaminopimelate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,978,378	0	T	C	100.0%	200.2 / NA	56	A174A (GCA→GCG)	lysA	diaminopimelate decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/29);  total (27/29)

TGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGCCATACCCGATGCCCTGGCGAAACCTGGCCCAG  >  NC_000913/2978241‑2978512                                                                                                                                          |                                                                                                                                       tGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAgcgc                                                                                                                                      >  1:652933/1‑140 (MQ=255) tGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATACCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAgcgc                                                                                                                                      >  1:71241/1‑140 (MQ=255)   tAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCgcgg                                                                                                                                    >  2:350123/1‑140 (MQ=255)         cTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCa                                                                                                                              <  1:42041/140‑1 (MQ=255)            aCCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGAt                                                                                                                           <  2:583424/140‑1 (MQ=255)            aCCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGAt                                                                                                                           <  2:1008994/140‑1 (MQ=255)            aCCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGAt                                                                                                                           <  1:954848/140‑1 (MQ=255)             ccGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATc                                                                                                                          >  2:635102/1‑140 (MQ=255)                aaTTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGgt                                                                                                                       <  2:892382/140‑1 (MQ=255)                aaTTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGgt                                                                                                                       <  2:673931/140‑1 (MQ=255)                aaTTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGgt                                                                                                                       <  2:532346/140‑1 (MQ=255)                aaTTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGgt                                                                                                                       <  2:381377/140‑1 (MQ=255)                aaTTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGgt                                                                                                                       <  1:845929/140‑1 (MQ=255)                  ttCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCg                                                                                                                         >  1:204263/1‑136 (MQ=255)                  ttCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCg                                                                                                                         <  2:204263/136‑1 (MQ=255)                      aTGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCa                                                                                                                 <  2:141202/140‑1 (MQ=255)                            tGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATAcc                                                                                                           <  2:404417/140‑1 (MQ=255)                            tGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATAcc                                                                                                           >  1:590877/1‑140 (MQ=255)                                    cATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGtgcttgc                                                                                                   >  1:409859/1‑140 (MQ=255)                                    cATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGtgcttgc                                                                                                   >  1:1092695/1‑140 (MQ=255)                                        gCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGtt                                                                                               <  2:652933/140‑1 (MQ=255)                                              cacacCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGcc                                                                                         >  2:540391/1‑140 (MQ=255)                                              cacacCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGcc                                                                                         >  1:672748/1‑140 (MQ=255)                                              cacacCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGcc                                                                                         >  2:1083374/1‑140 (MQ=255)                                                cacCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCAc                                                                                       <  1:540391/140‑1 (MQ=255)                                                 acCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCAcc                                                                                      >  2:849453/1‑140 (MQ=255)                                                 acCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCAcc                                                                                      >  2:6068/1‑140 (MQ=255)                                                 acCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCAcc                                                                                      <  1:6068/140‑1 (MQ=255)                                                     gTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTa                                                                                  <  1:350123/140‑1 (MQ=255)                                                       tCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTAtt                                                                                <  1:635102/140‑1 (MQ=255)                                                         cAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTgg                                                                              >  2:84935/1‑140 (MQ=255)                                                             tGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGtttt                                                                          <  1:84935/140‑1 (MQ=255)                                                                gCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTg                                                                       >  1:309696/1‑140 (MQ=255)                                                                gCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTg                                                                       >  1:48330/1‑140 (MQ=255)                                                                 cATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCAc                                                                                       >  2:565476/1‑123 (MQ=255)                                                                 cATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCAc                                                                                       <  1:565476/123‑1 (MQ=255)                                                                 cATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTgg                                                                      >  1:647576/1‑140 (MQ=255)                                                                          cGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCg                                                             <  1:1083374/140‑1 (MQ=255)                                                                               gAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCg                                                             <  2:16816/135‑1 (MQ=255)                                                                               gAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCg                                                             >  1:16816/1‑135 (MQ=255)                                                                                aaCCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCa                                                       >  1:160189/1‑140 (MQ=255)                                                                                aaCCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCa                                                       >  2:203820/1‑140 (MQ=255)                                                                                aaCCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTAGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCa                                                       >  1:608652/1‑140 (MQ=255)                                                                                     aTGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAAccc                                                  <  2:590877/140‑1 (MQ=255)                                                                                                aaTGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTAGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAAcgc                                       <  2:608652/140‑1 (MQ=255)                                                                                                 aTGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAAcgcg                                      <  2:309696/140‑1 (MQ=255)                                                                                                      gACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGcc                                 <  2:48330/140‑1 (MQ=255)                                                                                                      gACCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGcc                                 <  2:1092695/140‑1 (MQ=255)                                                                                                       aacggCTGCAGATGATGTTGTTGTATCACGTCCAGCGCAGCGGGCAGATAGGTGTACCAGATACCGTGCTTGCCGCTTTCGCCACCGGTATTGGTTTTTTGGATATGTCCGTGACCAATCCCCGGATTAACGCGCAGCCa                                <  2:71241/136‑1 (MQ=255)                                                                                                       aCCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAAcgcg                                      <  2:727655/134‑1 (MQ=255)                                                                                                       aCCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAAcgcg                                      >  1:727655/1‑134 (MQ=255)                                                                                                       aCCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGCCa                                >  1:973446/1‑140 (MQ=255)                                                                                                       aCCAGCTGCAGATGATGACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGCCa                                >  1:482253/1‑140 (MQ=255)                                                                                                                  atgatgACGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGCCATACCCGATAcc                     <  1:203820/140‑1 (MQ=255)                                                                                                                   tgatgaCGTTGTATCACGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGCCATACCCGATGccc                    >  2:1086651/1‑140 (MQ=255)                                                                                                                             gTATCACGTCCAGCCCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGCCATACCCGATGCCCTGGCGAAAcc          <  2:672748/140‑1 (MQ=255)                                                                                                                                   cGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGCCATACCCGATGCCCTGGCGAAACCTGGccc    >  2:1075170/1‑140 (MQ=255)                                                                                                                                   cGTCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGCCATACCCGATGCCCTGGCGAAACCTGGccc    >  1:566706/1‑140 (MQ=255)                                                                                                                                     tCCAGCGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGCCATACCCGATGCCCTGGCGAAACCTGGCCCAg  >  1:575206/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCCACCGGTATTGGTTTTTTGGCTATGTCCGTGACCAAACCCCGGATTAACGCGCAGCCATACCCGATGCCCTGGCGAAACCTGGCCCAG  >  NC_000913/2978241‑2978512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑