Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	987,008	(T)8→7	intergenic (‑26/+577)	ompF ← / ← asnS	outer membrane porin F/asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	987,001	0	T	.	100.0%	162.0 / NA	38	intergenic (‑19/+584)	ompF/asnS	outer membrane porin F/asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/19);  total (19/19)

TTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAA  >  NC_000913/986869‑987135                                                                                                                                     |                                                                                                                                       tttACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGttttttta                                                                                                                                 <  2:844781/140‑2 (MQ=255)  ttACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGtttttttat                                                                                                                                >  2:601058/1‑138 (MQ=255)       gTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGt                                                                                                                                        >  1:245811/1‑127 (MQ=255)       gTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGt                                                                                                                                        <  2:245811/127‑1 (MQ=255)             aTCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCa                                                                                                                    >  2:86121/1‑140 (MQ=255)             aTCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCa                                                                                                                    <  1:518806/140‑1 (MQ=255)             aTCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCa                                                                                                                    >  2:656581/1‑140 (MQ=255)             aTCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCa                                                                                                                    >  1:742628/1‑140 (MQ=255)             aTCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCa                                                                                                                    >  2:777363/1‑140 (MQ=255)               cTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACt                                                                                                                  <  1:549827/140‑1 (MQ=255)                tACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTg                                                                                                                 <  1:601058/140‑1 (MQ=255)                 tcTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCCCTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGc                                                                                                                <  1:67445/139‑1 (MQ=255)                  cTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTACGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGcc                                                                                                               >  2:359717/1‑140 (MQ=255)                       ttgCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCaa                                                                                                          >  2:496329/1‑140 (MQ=255)                        tgCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTCCCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAAt                                                                                                         <  1:234946/140‑1 (MQ=255)                            aTCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGcc                                                                                                               >  1:608985/1‑130 (MQ=255)                            aTCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGcc                                                                                                               <  2:608985/130‑1 (MQ=255)                               tttGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCt                                                                                                  <  1:595537/140‑1 (MQ=255)                                           ttCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGtt                                                                                                    >  2:257170/1‑126 (MQ=255)                                           ttCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGtt                                                                                                    <  1:257170/126‑1 (MQ=255)                                            tCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAAtt                                                                                     >  1:964644/1‑140 (MQ=255)                                              tGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTa                                                                                   <  2:742628/140‑1 (MQ=255)                                                   cGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGaa                                                                              <  1:86121/140‑1 (MQ=255)                                                             aCCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCACCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtgatga                                                                    >  2:462097/1‑140 (MQ=255)                                                                  ctactaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGtt                                                               <  1:778668/140‑1 (MQ=255)                                                                  ctactaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGtt                                                               >  2:778668/1‑140 (MQ=255)                                                                    actaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTg                                                             <  2:748402/140‑1 (MQ=255)                                                                    actaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTg                                                             <  1:656581/140‑1 (MQ=255)                                                                     ctaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTgg                                                            <  2:964644/140‑1 (MQ=255)                                                                      taACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGgt                                                           >  1:135882/1‑140 (MQ=255)                                                                            agCAGGGACGATCACTGCCAGAATATTGTGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCttt                                                     >  1:262511/1‑140 (MQ=255)                                                                            agCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCttt                                                     >  2:689254/1‑140 (MQ=255)                                                                                      aTCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGc                                           <  2:135882/140‑1 (MQ=255)                                                                                                 aaTATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTc                                >  2:213759/1‑140 (MQ=255)                                                                                                 aaTATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTc                                >  2:634358/1‑140 (MQ=255)                                                                                                 aaTATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTc                                >  1:831104/1‑140 (MQ=255)                                                                                                    atTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCAt                             <  1:496329/140‑1 (MQ=255)                                                                                                       gcgcTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCa                          >  2:414064/1‑140 (MQ=255)                                                                                                                       tattaCCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTa          >  1:736853/1‑140 (MQ=255)                                                                                                                             ccTCATGG‑TTTTTTTATGCCACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGa    <  1:777363/140‑1 (MQ=255)                                                                                                                             ccTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGa    <  2:831104/140‑1 (MQ=255)                                                                                                                               tCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGaaa  >  2:428709/1‑140 (MQ=255)                                                                                                                               tCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGaaa  <  1:689254/140‑1 (MQ=255)                                                                                                                               tCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGaaa  <  1:428709/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAA  >  NC_000913/986869‑987135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑