Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,030,704	+A	coding (268/2190 nt)	fadB ←	dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,030,701	1	.	A	100.0%	182.8 / NA	51	A91S (GCC→TCC)	fadB	dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (27/24);  total (27/24)

TGCGCAGATCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGC‑AAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTC  >  NC_000913/4030569‑4030833                                                                                                                                      |                                                                                                                                     tGCGCAGATCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGC‑aaaagtg                                                                                                                               <  2:551318/140‑5 (MQ=255)      aGATCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGcc                                                                                                                           <  1:905609/140‑1 (MQ=255)       gATCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCa                                                                                                                          <  2:856320/140‑1 (MQ=255)       gATCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCa                                                                                                                          <  2:694113/140‑1 (MQ=255)       gATCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCa                                                                                                                          <  2:820589/140‑1 (MQ=255)           cGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGa                                                                                                                      >  1:733595/1‑140 (MQ=255)             gCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACt                                                                                                                    >  1:520907/1‑140 (MQ=255)              cGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACtt                                                                                                                   <  2:767639/140‑1 (MQ=255)                tCGCCAGACGATAATCGGTCGCCAGCACGCACTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTaa                                                                                                                 <  1:306804/140‑1 (MQ=255)                  gCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACt                                                                                                               >  2:421682/1‑140 (MQ=255)                  gCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACt                                                                                                               >  1:651490/1‑140 (MQ=255)                   ccAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTg                                                                                                              <  1:178484/140‑1 (MQ=255)                   ccAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTg                                                                                                              <  1:996639/140‑1 (MQ=255)                   ccAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTACAGCCACTGACTTAACTg                                                                                                              <  1:112999/140‑1 (MQ=255)                           tAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAg                                                                                                      <  2:907981/140‑1 (MQ=255)                              tCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGaa                                                                                                   <  1:873969/140‑1 (MQ=255)                              tCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGaa                                                                                                   >  2:121853/1‑140 (MQ=255)                                   cGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAgg                                                                                              >  1:899177/1‑140 (MQ=255)                                     ccAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGaa                                                                                            >  1:862457/1‑140 (MQ=255)                                            gCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAg                                                                                                      >  2:1038410/1‑123 (MQ=255)                                            gCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAg                                                                                                      <  1:1038410/123‑1 (MQ=255)                                                   cAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAAtt                                                                              <  2:899177/140‑1 (MQ=255)                                                       cACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGt                                                                          <  2:897734/140‑1 (MQ=255)                                                       cACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGt                                                                          <  1:548796/140‑1 (MQ=255)                                                       cACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGt                                                                          <  2:563734/140‑1 (MQ=255)                                                        aCCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCg                                                                            <  1:732588/137‑1 (MQ=255)                                                        aCCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCg                                                                            >  2:732588/1‑137 (MQ=255)                                                            cccAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGatat                                                                     >  1:40955/1‑140 (MQ=255)                                                                    aTAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACcg                                                             <  1:121853/140‑1 (MQ=255)                                                                    aTAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACcg                                                             >  1:176603/1‑140 (MQ=255)                                                                    aTAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACcg                                                             <  2:733595/140‑1 (MQ=255)                                                                           ttGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATaa                                                      >  1:86835/1‑140 (MQ=255)                                                                                  cagcaATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGgctgc                                               >  2:675378/1‑140 (MQ=255)                                                                                   agcaATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGgctgct                                              >  2:620848/1‑140 (MQ=255)                                                                                       aTGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGt                                          >  2:903290/1‑140 (MQ=255)                                                                                          gTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCg                                       <  2:176603/140‑1 (MQ=255)                                                                                               cACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCCGCACCGACGATAAAGGCTGCTTTGTTc                                        <  2:96950/134‑1 (MQ=255)                                                                                               cACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTc                                        >  1:96950/1‑134 (MQ=255)                                                                                                    gCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCGCCGACGATAAAGGCTGCTTTGTTCGAACgcagcag                             >  2:722941/1‑140 (MQ=255)                                                                                                    gCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACgcagcag                             >  1:525637/1‑140 (MQ=255)                                                                                                    gCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACgcagcag                             >  2:246479/1‑140 (MQ=255)                                                                                                    gCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACgcagcag                             >  1:339665/1‑140 (MQ=255)                                                                                                        aTCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCagcagcagc                         <  2:520907/140‑1 (MQ=255)                                                                                                         tCTTCCAGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGcc                        >  2:668919/1‑140 (MQ=255)                                                                                                               aGGCGATTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTa                  >  1:382797/1‑140 (MQ=255)                                                                                                                    aTTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCt             >  1:992646/1‑140 (MQ=255)                                                                                                                    aTTAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGAGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCt             >  2:977179/1‑140 (MQ=255)                                                                                                                     ttAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTg            >  1:282151/1‑140 (MQ=255)                                                                                                                     ttAAACACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTg            >  2:420871/1‑140 (MQ=255)                                                                                                                       aaaCACGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGAt          <  1:722941/140‑1 (MQ=255)                                                                                                                         acacGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTg        <  2:862457/140‑1 (MQ=255)                                                                                                                         acacGCTATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTg        <  2:433444/140‑1 (MQ=255)                                                                                                                               tATTGGCAAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTc  >  2:52525/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     TGCGCAGATCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGC‑AAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTC  >  NC_000913/4030569‑4030833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑