Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,911,907	(T)6→7	coding (1745/2235 nt)	relA ←	GDP/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,911,901	1	.	T	100.0%	117.8 / NA	35	S584N (AGC→AAC)	relA	GDP/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (18/17);  total (18/17)

CCGCGCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGC‑TTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGATATCCCCGCCACCAATCGCCGCCAGCAAC  >  NC_000913/2911763‑2912029                                                                                                                                            |                                                                                                                                 ccGCGCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGC‑t                                                                                                                                 >  1:617192/1‑140 (MQ=255)       cccTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGC‑ttttttt                                                                                                                           <  2:142142/140‑2 (MQ=255)        ccTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTATAGC‑tttttttg                                                                                                                          <  1:821588/140‑3 (MQ=255)          tGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGC‑tttttttgct                                                                                                                        <  1:607679/140‑5 (MQ=255)               aaTGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGt                                                                                                                    <  2:426848/140‑1 (MQ=255)                 tGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTg                                                                                                                  >  1:336118/1‑140 (MQ=255)                   aaGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGt                                                                                                                    <  2:786132/136‑1 (MQ=255)                   aaGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGt                                                                                                                    >  1:786132/1‑136 (MQ=255)                   aaGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCt                                                                                                                >  1:170524/1‑140 (MQ=255)                    aGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCtt                                                                                                               <  1:712793/140‑1 (MQ=255)                      ccGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCa                                                                                                             >  1:57757/1‑140 (MQ=255)                              ctcatctcCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCgcggcg                                                                                                     <  2:57757/140‑1 (MQ=255)                              ctcatctcCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCgcggcg                                                                                                     <  2:141783/140‑1 (MQ=255)                              ctcatctcCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCgcggcg                                                                                                     <  2:617192/140‑1 (MQ=255)                                 atctcCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGGAGCTTTTTTTGCTGAAGATGCTTCAGCgcggcggcg                                                                                                  >  1:914151/1‑140 (MQ=255)                                         gAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGTCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGctc                                                                                          >  2:288638/1‑140 (MQ=255)                                                  ggCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACt                                                                                 <  2:509110/140‑1 (MQ=255)                                                      gcagcGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGAGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGc                                                                             <  2:717389/140‑1 (MQ=255)                                                                         aTCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCa                                                          >  1:582234/1‑140 (MQ=255)                                                                            aGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGa                                                       <  2:1014914/140‑1 (MQ=255)                                                                             ggTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGaa                                                      >  1:746643/1‑140 (MQ=255)                                                                                tGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGtt                                                   <  2:170524/140‑1 (MQ=255)                                                                                tGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGtt                                                   <  1:410768/140‑1 (MQ=255)                                                                                  ccAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCa                                                 <  2:336118/140‑1 (MQ=255)                                                                                    aaCACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCAcc                                               <  2:582234/140‑1 (MQ=255)                                                                                            cGACTA‑CACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTg                                      >  2:268600/1‑140 (MQ=255)                                                                                              aCTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTga                                     <  2:746643/140‑1 (MQ=255)                                                                                                tACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTgaga                                   >  2:408969/1‑140 (MQ=255)                                                                                                     cgcgACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGat                              >  1:182858/1‑140 (MQ=255)                                                                                                     cgcgACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGat                              >  2:611296/1‑140 (MQ=255)                                                                                                      gcgACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGata                             <  1:611296/140‑1 (MQ=255)                                                                                                       cgACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGatat                            >  2:814101/1‑140 (MQ=255)                                                                                                                    tACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGATATCCCCGCCACCAAt               >  2:1007541/1‑140 (MQ=255)                                                                                                                    tACTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGATATCCCCGCCACCAAt               >  2:681120/1‑140 (MQ=255)                                                                                                                      cTGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGATATCCCCGCCACCAATcg             <  1:681120/140‑1 (MQ=255)                                                                                                                       tGCGGTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGATATCCCCGCCACCAATcgc            >  2:256441/1‑140 (MQ=255)                                                                                                                           gTTTTGCGGCGTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGATATCCCCGCCACCAATCgccgcc        >  1:389805/1‑140 (MQ=255)                                                                                                                              ttGCGGCGTGGAGCTTTTTTTGCTTAAGTTGCTTCAGCGCGGCGGCGGCCTGCTCTTCTGCACTCGGCTTATTAAATTGCGATTGCCGGAAGGTCACCATCTTATTGAGACGGATATCCCCGCCACCAATCGccgcccgc     >  2:161022/1‑140 (MQ=255)                                                                                                                                 cggcgTGTAGCTTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGATATCCCCGCCACCAATCGCCGCCAGCAAc  <  2:389805/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                 CCGCGCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGC‑TTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACTCGGCTTATTAAATTGCGATTGCAGGAAGTTCACCATCTGATTGAGACGGATATCCCCGCCACCAATCGCCGCCAGCAAC  >  NC_000913/2911763‑2912029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑