Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	407,822	T→C	V132A (GTG→GCG)	aroM →	protein AroM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	407,822	0	T	C	100.0%	222.6 / NA	62	V132A (GTG→GCG)	aroM	protein AroM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/25);  total (37/25)

ATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGATGCCGGGAAAG  >  NC_000913/407699‑407956                                                                                                                            |                                                                                                                                       aTGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCg                                                                                                                        <  2:61446/140‑1 (MQ=255)  tGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCgg                                                                                                                       >  1:759754/1‑140 (MQ=255)   gACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGt                                                                                                                      <  2:318370/140‑1 (MQ=255)   gACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGt                                                                                                                      <  2:331291/140‑1 (MQ=255)   gACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGt                                                                                                                      <  2:564934/140‑1 (MQ=255)       tCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTgag                                                                                                                  >  2:558811/1‑140 (MQ=255)       tCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTgag                                                                                                                  >  1:217237/1‑140 (MQ=255)                      gTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGtt                                                                                                   <  2:210741/140‑1 (MQ=255)                       taaaGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTc                                                                                                  >  1:671668/4‑140 (MQ=255)                       tACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTc                                                                                                  >  2:768271/1‑140 (MQ=255)                             aaaCATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGgcgc                                                                                            >  2:564755/1‑140 (MQ=255)                               aCATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCaa                                                                                          >  1:101211/1‑140 (MQ=255)                               aCATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCaa                                                                                          >  1:943615/1‑140 (MQ=255)                                 aTTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCaaaa                                                                                        >  2:425641/1‑140 (MQ=255)                                    agtagtATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATg                                                                                     >  1:949449/1‑140 (MQ=255)                                    agtagtATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTG‑AGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATgg                                                                                    >  1:992879/1‑140 (MQ=255)                                           tGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAAtt                                                                              >  1:22121/1‑140 (MQ=255)                                             aCTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAAtttt                                                                            >  1:948429/1‑140 (MQ=255)                                             aCTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAAtttt                                                                            >  1:1024240/1‑140 (MQ=255)                                                gcgcGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCa                                                                         >  1:297570/1‑140 (MQ=255)                                                 cgcgTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAg                                                                        >  2:81057/1‑140 (MQ=255)                                                 cgcgTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAg                                                                        >  2:884607/1‑140 (MQ=255)                                                   cgTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGaa                                                                      >  1:731637/1‑140 (MQ=255)                                                   cgTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGaa                                                                      <  2:297570/140‑1 (MQ=255)                                                       aTACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCg                                                                  <  2:1024240/140‑1 (MQ=255)                                                        tACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGc                                                                 >  2:139204/1‑140 (MQ=255)                                                        tACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGc                                                                 <  1:139204/140‑1 (MQ=255)                                                         aCGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGcc                                                                <  1:876886/140‑1 (MQ=255)                                                           gATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCgg                                                              >  1:375943/1‑140 (MQ=255)                                                                ttcttGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTAttt                                                         >  1:583102/1‑140 (MQ=255)                                                                ttcttGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTAttt                                                         >  2:765451/1‑140 (MQ=255)                                                                   ttGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCa                                                      <  1:768271/140‑1 (MQ=255)                                                                   ttGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCa                                                      <  1:425641/140‑1 (MQ=255)                                                                   ttGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCa                                                      <  2:250577/140‑1 (MQ=255)                                                                        ccGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTggg                                                 <  2:375943/140‑1 (MQ=255)                                                                        ccGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTggg                                                 >  1:42682/1‑140 (MQ=255)                                                                         cGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGt                                                <  2:949449/140‑1 (MQ=255)                                                                         cGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGt                                                <  2:943615/140‑1 (MQ=255)                                                                          gTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTa                                               <  1:998704/140‑1 (MQ=255)                                                                          gTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTa                                               <  2:731637/140‑1 (MQ=255)                                                                           tCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTaa                                              <  1:81057/140‑1 (MQ=255)                                                                           tCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTT‑AGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGCAAc                                             >  1:467184/1‑140 (MQ=255)                                                                                  tatTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCAtt                                       <  2:217237/140‑1 (MQ=255)                                                                                        cTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTT‑AGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGAtt                                <  2:467184/140‑1 (MQ=255)                                                                                           cACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAAcccc                                          >  2:420345/1‑128 (MQ=255)                                                                                           cACTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAAcccc                                          <  1:420345/128‑1 (MQ=255)                                                                                            aCTGGTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAg                             >  2:596209/1‑140 (MQ=255)                                                                                               ggTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAAc                          >  2:792602/1‑140 (MQ=255)                                                                                                gTTTCCTCTATTGTTGAAGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACa                         >  1:811178/1‑140 (MQ=255)                                                                                                                 aGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCAtt               <  1:128644/133‑1 (MQ=255)                                                                                                                 aGATCATCAGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCAtt               >  2:128644/1‑133 (MQ=255)                                                                                                                    tcatcaGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGATGCCg        >  1:159803/1‑137 (MQ=255)                                                                                                                    tcatcaGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGATGCCg        <  2:159803/137‑1 (MQ=255)                                                                                                                    tcatcaGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGATGCCGGGa     >  2:898865/1‑140 (MQ=255)                                                                                                                    tcatcaGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGATGCCGGGa     >  1:469923/1‑140 (MQ=255)                                                                                                                     catcaGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGATGCCGGGaa    >  1:109971/1‑140 (MQ=255)                                                                                                                     catcaGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGATGCCGGGaa    >  2:611364/1‑140 (MQ=255)                                                                                                                      atcaGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGATGCCGGGaaa   <  1:541445/140‑1 (MQ=255)                                                                                                                       tcaGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGa             <  2:777223/129‑1 (MQ=255)                                                                                                                       tcaGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGa             >  1:777223/1‑129 (MQ=255)                                                                                                                       tcaGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGATGCCGGGAAAg  >  1:493548/1‑140 (MQ=255)                                                                                                                       tcaGGCGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGATGCCGGGAAAg  >  1:741525/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                       ATGACGTCATTTTATTAATGAGTACAGCAAACATTAGTAGTATGACTGCGCGTAATACGATCTTTCTTGAGCCGTCGCGAATATTGCCTCCACTGGTTTCCTCTATTGTTGAAGATCATCAGGTGGGGGTTATCGTTCCGGTTGAGGAGATGCTGCCCGTTCAGGCGCAAAAATGGCAAATTTTGCAGAAATCGCCGGTATTTTCATTGGGTAACCCCATTCATGATTCAGAACAAAAAATCATTGATGCCGGGAAAG  >  NC_000913/407699‑407956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑