Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,386,758	G→A	F477F (TTC→TTT)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,386,758	0	G	A	100.0%	207.2 / NA	58	F477F (TTC→TTT)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/36);  total (22/36)

GCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATG  >  NC_000913/3386621‑3386893                                                                                                                                          |                                                                                                                                        gCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACtgaaatgaaa                                                                                                                                       >  1:488887/1‑140 (MQ=255)      aGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGTCGATTTGCCCTAATGCGCTGTCAAGAAACTGACTGAAATGAAAAGTCa                                                                                                                                  <  2:101923/140‑1 (MQ=255)      aGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCa                                                                                                                                  <  1:887616/140‑1 (MQ=255)        aaacaaacTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATc                                                                                                                                <  2:161310/140‑1 (MQ=255)                     aaGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTATTGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCa                                                                                                                   <  2:85614/140‑1 (MQ=255)                     aaGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCa                                                                                                                   <  1:203728/140‑1 (MQ=255)                     aaGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCa                                                                                                                   <  1:221350/140‑1 (MQ=255)                     aaGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCa                                                                                                                   <  2:488887/140‑1 (MQ=255)                       gCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACg                                                                                                                 <  1:1026951/140‑1 (MQ=255)                          gTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGata                                                                                                              >  1:67836/1‑140 (MQ=255)                            acacGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGataat                                                                                                            >  2:100880/1‑140 (MQ=255)                            acacGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGataat                                                                                                            <  2:52451/140‑1 (MQ=255)                            acacGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGat                                                                                                               <  1:738871/137‑1 (MQ=255)                            acacGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGat                                                                                                               >  2:738871/1‑137 (MQ=255)                             cacGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGataata                                                                                                           >  1:117900/1‑140 (MQ=255)                                gTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATAttt                                                                                                        >  1:236820/1‑140 (MQ=255)                                 tCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTa                                                                                                       <  2:839581/140‑1 (MQ=255)                                 tCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTa                                                                                                       <  2:731959/140‑1 (MQ=255)                                  ccGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTAt                                                                                                      >  1:624026/1‑140 (MQ=255)                                   cGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATg                                                                                                     <  2:492897/140‑1 (MQ=255)                                     gTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATAtttg                                                                                                       <  1:98323/136‑2 (MQ=255)                                     gTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATAtttg                                                                                                       >  2:98323/1‑135 (MQ=255)                                     gTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGt                                                                                                   >  2:741852/1‑140 (MQ=255)                                     gTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGt                                                                                                   >  2:241933/1‑140 (MQ=255)                                     gTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGt                                                                                                   >  2:850113/1‑140 (MQ=255)                                         tGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTg                                                                                               <  1:397263/140‑1 (MQ=255)                                           tCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCATGATAATATTTATGGTGCTGGc                                                                                             >  2:956219/1‑140 (MQ=255)                                            cgcgcgATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGcc                                                                                            <  2:236820/140‑1 (MQ=255)                                            cgcgcgATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGcc                                                                                            >  1:121237/1‑140 (MQ=255)                                              cgcgATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAg                                                                                          <  2:197755/140‑1 (MQ=255)                                                cgATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGTCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTg                                                                                        >  2:1006058/1‑140 (MQ=255)                                                cgATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTg                                                                                        >  1:218103/1‑140 (MQ=255)                                                       tCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGcc                                                                                      >  2:686371/1‑135 (MQ=255)                                                       tCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGcc                                                                                      <  1:686371/135‑1 (MQ=255)                                                                   aaaaTAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCg                                                                     <  1:241933/140‑1 (MQ=255)                                                                    aaaTAACGGTGAACGCGAGCACACAGTCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGc                                                                    <  1:1006058/140‑1 (MQ=255)                                                                    aaaTAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGc                                                                    <  1:360357/140‑1 (MQ=255)                                                                    aaaTAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGc                                                                    <  2:367944/140‑1 (MQ=255)                                                                            gTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTg                                                            <  2:117900/140‑1 (MQ=255)                                                                            gTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTg                                                            <  2:870755/140‑1 (MQ=255)                                                                            gTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTg                                                            <  1:6688/140‑1 (MQ=255)                                                                            gTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTg                                                            <  2:624026/140‑1 (MQ=255)                                                                            gTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTg                                                            <  1:793663/140‑1 (MQ=255)                                                                            gTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTg                                                            <  2:219004/140‑1 (MQ=255)                                                                            gTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTg                                                            <  2:121237/140‑1 (MQ=255)                                                                               aaCGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTAc                                                         >  2:395319/1‑140 (MQ=255)                                                                               aaCGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTAc                                                         >  2:665034/1‑140 (MQ=255)                                                                                    gAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCta                                                    <  1:956219/140‑1 (MQ=255)                                                                                         cacaGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCg                                               <  1:665034/140‑1 (MQ=255)                                                                                          acaGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGa                                              <  1:850113/140‑1 (MQ=255)                                                                                            aGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTGATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAgg                                            >  2:940527/1‑140 (MQ=255)                                                                                              ccGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGgaa                                          >  2:93891/1‑140 (MQ=255)                                                                                                  cgaTTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAAt                                      <  1:18762/140‑1 (MQ=255)                                                                                                       tGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAg                                 <  1:93891/140‑1 (MQ=255)                                                                                                          ccTAATGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCAc                              <  1:940527/140‑1 (MQ=255)                                                                                                              aTGCGCTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGcc                          >  2:624734/1‑140 (MQ=255)                                                                                                                 cgcTGTCGAGAAACTGACTGAAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAgg                       >  1:817277/1‑140 (MQ=255)                                                                                                                                gactgaAATGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACagcagc        >  1:513463/1‑140 (MQ=255)                                                                                                                                      aaTGAAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATg  <  1:100880/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATG  >  NC_000913/3386621‑3386893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑