Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,463,649	T→C	A242A (GCT→GCC)	gspK →	Type II secretion system protein GspK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,463,649	0	T	C	100.0%	279.2 / NA	77	A242A (GCT→GCC)	gspK	Type II secretion system protein GspK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/36);  total (41/36)

GGCTTATCCCGTATGTCTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCT  >  NC_000913/3463525‑3463785                                                                                                                             |                                                                                                                                         ggCTTATCCCGTATGTCTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCaa                                                                                                                           >  2:857506/1‑140 (MQ=255)        cccGTATGTCTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCGGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGc                                                                                                                    <  1:584166/140‑1 (MQ=255)          cGTATGTCTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCg                                                                                                                  <  2:535686/140‑1 (MQ=255)             aTGTCTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAgg                                                                                                               >  2:1005104/1‑140 (MQ=255)              tGTCTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTGACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAggg                                                                                                              <  1:866598/140‑1 (MQ=255)              tGTCTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAggg                                                                                                              <  1:609533/140‑1 (MQ=255)                 cTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAg                                                                                                           >  2:370951/1‑140 (MQ=255)                 cTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAg                                                                                                           >  2:974396/1‑140 (MQ=255)                 cTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAg                                                                                                           <  1:167450/140‑1 (MQ=255)                 cTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAg                                                                                                           >  2:818906/1‑140 (MQ=255)                  tGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAgg                                                                                                               >  1:627598/1‑135 (MQ=255)                  tGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAgg                                                                                                               <  2:627598/135‑1 (MQ=255)                  tGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAgg                                                                                                          >  1:113709/1‑140 (MQ=255)                  tGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAgg                                                                                                          >  2:109500/1‑140 (MQ=255)                    cGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAggct                                                                                                        >  1:562495/1‑140 (MQ=255)                    cGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAggct                                                                                                        >  2:1037301/1‑140 (MQ=255)                     gTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACTGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAggctg                                                                                                       >  2:299008/1‑140 (MQ=255)                      ttCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAggctgg                                                                                                      <  2:562495/140‑1 (MQ=255)                         tcCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCCCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTa                                                                                                   <  2:137318/140‑1 (MQ=255)                          cccGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTaa                                                                                                  >  2:513629/1‑140 (MQ=255)                              aCCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCAc                                                                                              >  2:19888/1‑140 (MQ=255)                              aCCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCAc                                                                                              >  1:692826/1‑140 (MQ=255)                                   ggAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATATCATCTCGGATGCTGACGCGCGGGTCTTATTGCAAAAAAGGCCGAGGGTATGTGGGCTAACCACGGATg                                                                                         <  2:54051/140‑1 (MQ=255)                                   ggAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTACCCACGGATg                                                                                         <  2:788165/140‑1 (MQ=255)                                   ggAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATg                                                                                         <  2:692826/140‑1 (MQ=255)                                     aaCTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCa                                                                                       <  1:974396/140‑1 (MQ=255)                                     aaCTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCa                                                                                       <  2:113709/140‑1 (MQ=255)                                                 aTCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTgg                                                                           >  1:135643/1‑140 (MQ=255)                                                    ttAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGgcg                                                                        <  1:19888/140‑1 (MQ=255)                                                          tGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGcagcag                                                                  <  1:315950/140‑1 (MQ=255)                                                            cTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGa                                                                >  1:136169/1‑140 (MQ=255)                                                            cTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGa                                                                >  1:401224/1‑140 (MQ=255)                                                            cTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGa                                                                >  2:1007216/1‑140 (MQ=255)                                                            cTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGa                                                                >  2:956014/1‑140 (MQ=255)                                                            cTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGa                                                                >  1:619660/1‑140 (MQ=255)                                                             tAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGAt                                                               >  2:161145/1‑140 (MQ=255)                                                              aaCGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGAtt                                                              >  1:308479/1‑140 (MQ=255)                                                                cGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTtc                                                            >  1:645536/1‑140 (MQ=255)                                                                cGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTtc                                                            >  2:403340/1‑140 (MQ=255)                                                                  gagaACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGTGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTtctc                                                          <  2:619660/140‑1 (MQ=255)                                                                  gagaACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTtctc                                                          <  2:303810/140‑1 (MQ=255)                                                                  gagaACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTtctc                                                          <  1:857506/140‑1 (MQ=255)                                                                   agaACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTcc                                                         <  1:1005104/140‑1 (MQ=255)                                                                        gATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGgtgt                                                    <  1:818906/140‑1 (MQ=255)                                                                         atatTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGtgtg                                                   <  2:135643/140‑1 (MQ=255)                                                                         atatTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGtgtg                                                   <  2:645536/140‑1 (MQ=255)                                                                         atatTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGtgtg                                                   <  1:252512/140‑1 (MQ=255)                                                                         atatTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGtgtg                                                   >  2:980820/1‑140 (MQ=255)                                                                             tCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGc                                               <  2:136169/140‑1 (MQ=255)                                                                                 ctctTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACt                                           <  2:308479/140‑1 (MQ=255)                                                                                 ctctTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACt                                           >  1:1016274/1‑140 (MQ=255)                                                                                 ctctTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACt                                           <  1:109500/140‑1 (MQ=255)                                                                                  tctTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTg                                          >  2:360130/1‑140 (MQ=255)                                                                                    ttCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGt                                        >  1:815944/1‑140 (MQ=255)                                                                                      cAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTg                                      <  2:815944/140‑1 (MQ=255)                                                                                         agCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGc                                               >  1:278133/1‑128 (MQ=255)                                                                                         agCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGc                                               <  2:278133/128‑1 (MQ=255)                                                                                         agCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTc                                   >  1:988962/1‑140 (MQ=255)                                                                                         agCGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGAAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTc                                   <  1:513629/140‑1 (MQ=255)                                                                                           cGTTATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAg                                 >  2:635197/1‑140 (MQ=255)                                                                                              tATTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTg                              >  1:72259/1‑140 (MQ=255)                                                                                               aTTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGa                             <  2:312567/140‑1 (MQ=255)                                                                                               aTTCCTCAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGa                             >  2:756137/1‑140 (MQ=255)                                                                                                     cAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGACGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGc                       >  2:151302/1‑140 (MQ=255)                                                                                                     cAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGc                       <  1:635197/140‑1 (MQ=255)                                                                                                     cAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGc                       <  1:756137/140‑1 (MQ=255)                                                                                                     cAATAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGc                       <  1:941599/140‑1 (MQ=255)                                                                                                       aTAACATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCAt                     <  1:370951/140‑1 (MQ=255)                                                                                                          aCATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCAtctc                  >  2:737816/1‑140 (MQ=255)                                                                                                           cATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGc                       >  2:381470/1‑134 (MQ=255)                                                                                                           cATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGc                       <  1:381470/134‑1 (MQ=255)                                                                                                           cATCACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATctct                 <  1:956014/140‑1 (MQ=255)                                                                                                              cACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCa                      >  2:373113/1‑132 (MQ=255)                                                                                                              cACGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCa                      <  1:373113/132‑1 (MQ=255)                                                                                                               aCGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTcc             <  2:988962/140‑1 (MQ=255)                                                                                                                cGGATGCTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTccc            >  1:499675/1‑140 (MQ=255)                                                                                                                      cTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGc      >  1:894457/1‑140 (MQ=255)                                                                                                                      cTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGc      >  1:332634/1‑140 (MQ=255)                                                                                                                      cTGACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGc      >  1:1045394/1‑140 (MQ=255)                                                                                                                        gACGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCg    <  2:332634/140‑1 (MQ=255)                                                                                                                          cGCCCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCt  <  1:864525/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                         GGCTTATCCCGTATGTCTGCGTTCTCCCGACCACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTTCTTTACTGGGCGCAGCAGGATTTCTCCGGTGTGAAGCCACTGGTTGCTCAGGTGAAAAGGCATCTCTTTCCCTATAGCCGCT  >  NC_000913/3463525‑3463785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑