Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,520,593	C→T	L37L (TTG→TTA)	hofQ ←	DNA utilization protein HofQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,520,593	0	C	T	100.0%	301.1 / NA	84	L37L (TTG→TTA)	hofQ	DNA utilization protein HofQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/40);  total (44/40)

TTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAAC  >  NC_000913/3520460‑3520729                                                                                                                                      |                                                                                                                                         ttATCAGTCCGGCGCTTTTCACTACAGTTTGTAGCGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACAcc                                                                                                                                    >  1:794831/1‑140 (MQ=255)       gTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCt                                                                                                                              >  1:946039/1‑140 (MQ=255)       gTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCt                                                                                                                              >  2:823045/1‑140 (MQ=255)           ggCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCg                                                                                                                          <  2:987267/140‑1 (MQ=255)           ggCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCg                                                                                                                          <  2:794831/140‑1 (MQ=255)           ggCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCg                                                                                                                          >  1:860530/1‑140 (MQ=255)            gcgcTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCgg                                                                                                                         >  1:717523/1‑140 (MQ=255)             cgcTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACTGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGa                                                                                                                        <  1:769430/140‑1 (MQ=255)               cTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAAc                                                                                                                      <  2:252779/140‑1 (MQ=255)                 tttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGt                                                                                                                    <  1:39217/140‑1 (MQ=255)                 tttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGt                                                                                                                    <  2:714466/140‑1 (MQ=255)                   tCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCa                                                                                                                  <  1:591829/140‑1 (MQ=255)                         cAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATccacc                                                                                                            >  1:696987/1‑140 (MQ=255)                           gTTTGTAGTGCCTGCTTCCATGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCAt                                                                                                          <  1:684206/140‑1 (MQ=255)                            tttGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATc                                                                                                                >  1:128959/1‑133 (MQ=255)                            tttGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATc                                                                                                                <  2:128959/133‑1 (MQ=255)                            tttGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATc                                                                                                         >  2:902676/1‑140 (MQ=255)                              tGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAg                                                                                                       >  1:533220/1‑140 (MQ=255)                                 aGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGt                                                                                                    >  2:609874/1‑140 (MQ=255)                                 aGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGt                                                                                                    >  2:465259/1‑140 (MQ=255)                                   tGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCa                                                                                                  >  1:236465/1‑140 (MQ=255)                                   tGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCa                                                                                                  >  2:323545/1‑140 (MQ=255)                                   tGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCa                                                                                                  >  1:41890/1‑140 (MQ=255)                                    gCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCAc                                                                                                 <  2:717523/140‑1 (MQ=255)                                     ccTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACt                                                                                                >  1:968169/1‑140 (MQ=255)                                     ccTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACt                                                                                                >  1:787044/1‑140 (MQ=255)                                        gCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTGAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACtttt                                                                                             >  2:954209/1‑140 (MQ=255)                                                   aCATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTgc                                                                                  <  1:823045/140‑1 (MQ=255)                                                   aCATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTgc                                                                                  <  1:604945/140‑1 (MQ=255)                                                    cATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTgcc                                                                                 >  1:993785/1‑140 (MQ=255)                                                    cATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTgcc                                                                                 >  1:1010916/1‑140 (MQ=255)                                                    cATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTgcc                                                                                 >  1:498720/1‑140 (MQ=255)                                                     aTCTGTTAGATGTAACGACACCGTGCCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTgcc                                                                                 >  2:510859/1‑139 (MQ=255)                                                     aTCTGTTAGATGTAACGACACCGTGCCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTgcc                                                                                 <  1:510859/139‑1 (MQ=255)                                                            aGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTAc                                                                         >  2:1022848/1‑140 (MQ=255)                                                             gATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACg                                                                        <  1:609874/140‑1 (MQ=255)                                                              aTGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGc                                                                       >  1:867414/1‑140 (MQ=255)                                                                  aaCGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGcc                                                                      >  1:546958/1‑137 (MQ=255)                                                                  aaCGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGcc                                                                      <  2:546958/137‑1 (MQ=255)                                                                  aaCGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCggg                                                                   >  1:19419/1‑140 (MQ=255)                                                                    cGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACg                                                                        >  2:435338/1‑133 (MQ=255)                                                                    cGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACg                                                                        <  1:435338/133‑1 (MQ=255)                                                                    cGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTa                                                                 >  2:681207/1‑140 (MQ=255)                                                                    cGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTa                                                                 >  2:401090/1‑140 (MQ=255)                                                                      acacCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATc                                                               <  1:465259/140‑1 (MQ=255)                                                                         ccGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGcc                                                                      >  2:670774/1‑130 (MQ=255)                                                                         ccGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGcc                                                                      <  1:670774/130‑1 (MQ=255)                                                                         ccGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGc                                                            <  2:1008223/140‑1 (MQ=255)                                                                         ccGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGc                                                            <  1:324982/140‑1 (MQ=255)                                                                         ccGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGc                                                            >  2:25884/1‑140 (MQ=255)                                                                          cGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCa                                                           >  2:266447/1‑140 (MQ=255)                                                                            tACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATc                                                         >  1:492956/1‑140 (MQ=255)                                                                            tACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATc                                                         >  2:940209/1‑140 (MQ=255)                                                                            tACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATc                                                         >  2:230242/1‑140 (MQ=255)                                                                            tACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATc                                                         <  2:41890/140‑1 (MQ=255)                                                                              ccGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCaa                                                       <  1:1022848/140‑1 (MQ=255)                                                                                 cTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACag                                                    >  1:646979/1‑140 (MQ=255)                                                                                    aCGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACagtag                                                 >  1:732434/1‑140 (MQ=255)                                                                                         tGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCa                                                              <  1:695396/122‑1 (MQ=255)                                                                                         tGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCa                                                              >  2:695396/1‑122 (MQ=255)                                                                                         tGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCgg                                            <  1:377764/140‑1 (MQ=255)                                                                                           gCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCt                                          >  2:129342/1‑140 (MQ=255)                                                                                           gCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCt                                          >  2:629329/1‑140 (MQ=255)                                                                                                     cAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCt                                <  1:401090/140‑1 (MQ=255)                                                                                                      aGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCtt                               <  2:787044/140‑1 (MQ=255)                                                                                                       ggTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTc                              >  2:829230/1‑140 (MQ=255)                                                                                                       ggTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTc                              <  1:266447/140‑1 (MQ=255)                                                                                                       ggTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTc                              <  2:696987/140‑1 (MQ=255)                                                                                                        gTTCAACTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCa                             <  1:859678/140‑1 (MQ=255)                                                                                                             aCTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGt                        <  1:856058/140‑1 (MQ=255)                                                                                                             aCTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGt                        <  1:120784/140‑1 (MQ=255)                                                                                                              cTTCTCCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTa                       <  2:533220/140‑1 (MQ=255)                                                                                                                  tcCTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTAtctc                   >  2:543528/1‑140 (MQ=255)                                                                                                                   ccTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTcc                  >  1:847442/1‑140 (MQ=255)                                                                                                                   ccTGTTCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTcc                  >  2:569632/1‑140 (MQ=255)                                                                                                                        tCAGCCAGCGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGcc             >  2:433551/1‑140 (MQ=255)                                                                                                                         cagccagcGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCg            <  2:19419/140‑1 (MQ=255)                                                                                                                         cagccagcGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCg            <  2:860530/140‑1 (MQ=255)                                                                                                                           gccagcGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGtt          <  1:230242/140‑1 (MQ=255)                                                                                                                           gccagcGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGtt          <  1:940209/140‑1 (MQ=255)                                                                                                                             cagcGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGc        <  2:732434/140‑1 (MQ=255)                                                                                                                             cagcGCCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGc        <  2:993785/140‑1 (MQ=255)                                                                                                                                cgcCTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCcac     <  2:968169/140‑1 (MQ=255)                                                                                                                                   cTGTAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAAc  <  2:732166/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGCGTCACTTTTTGCGGCTTTGCCGCCTGTACGCCGGGTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAAC  >  NC_000913/3520460‑3520729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑