Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,079,836	A→G	D29G (GAC→GGC)	yiiF →	protein YiiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,079,836	0	A	G	100.0%	346.4 / NA	94	D29G (GAC→GGC)	yiiF	protein YiiF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/45);  total (49/45)

ATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAA  >  NC_000913/4079699‑4079969                                                                                                                                          |                                                                                                                                      aTTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGcc                                                                                                                                     >  1:197220/1‑140 (MQ=255)    cAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTAt                                                                                                                                  >  2:204867/1‑140 (MQ=255)      aaaaTGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTa                                                                                                                                >  1:939575/1‑140 (MQ=255)      aaaaTGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAACTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTa                                                                                                                                >  1:44001/1‑140 (MQ=255)           gATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGa                                                                                                                           <  1:111048/140‑1 (MQ=255)           gATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGa                                                                                                                           >  2:638126/1‑140 (MQ=255)            atatTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGaa                                                                                                                          >  1:485473/1‑140 (MQ=255)               ttGGCGAGAGATTTATGAACTCTCTGGCAGGTATAGGTATGGGGAGAATTTGACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCg                                                                                                                       >  2:1041588/1‑140 (MQ=255)                 ggCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGt                                                                                                                     <  2:485473/140‑1 (MQ=255)                     agaTATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGAt                                                                                                                 >  1:1015395/1‑140 (MQ=255)                     agaTATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGAt                                                                                                                 <  2:241324/140‑1 (MQ=255)                        tatTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCaa                                                                                                              <  1:18474/140‑1 (MQ=255)                           ttATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATAc                                                                                                           <  2:412595/140‑1 (MQ=255)                            tATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAg                                                                                                                         <  1:293668/125‑1 (MQ=255)                            tATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAg                                                                                                                         >  2:293668/1‑125 (MQ=255)                                aaCTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGAt                                                                                                      <  1:170095/140‑1 (MQ=255)                                  ctctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATaa                                                                                                    >  2:407093/1‑140 (MQ=255)                                  ctctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATaa                                                                                                    >  1:630926/1‑140 (MQ=255)                                  ctctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATaa                                                                                                    >  2:989938/1‑140 (MQ=255)                                    ctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAaat                                                                                                  >  1:259727/1‑140 (MQ=255)                                     tctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAaatc                                                                                                 >  1:859589/1‑140 (MQ=255)                                      ctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAaatca                                                                                                >  2:899071/1‑140 (MQ=255)                                              tataGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCaaa                                                                                        <  2:419511/140‑1 (MQ=255)                                              tataGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCaaa                                                                                        <  2:549324/140‑1 (MQ=255)                                                   atatGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCa                                                                                   <  1:204867/140‑1 (MQ=255)                                                       gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGaa                                                                               >  2:679038/1‑140 (MQ=255)                                                       gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGaa                                                                               >  1:1047154/1‑140 (MQ=255)                                                       gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGaa                                                                               >  2:427278/1‑140 (MQ=255)                                                        ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                              >  1:535520/1‑140 (MQ=255)                                                        ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                              >  1:375554/1‑140 (MQ=255)                                                        ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                              >  1:817647/1‑140 (MQ=255)                                                        ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                              >  2:998052/1‑140 (MQ=255)                                                        ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                              >  2:160779/1‑140 (MQ=255)                                                        ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                              >  2:751889/1‑140 (MQ=255)                                                        ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                                              >  1:754132/1‑140 (MQ=255)                                                         gCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAcc                                                                             <  1:998052/140‑1 (MQ=255)                                                          cAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCa                                                                            <  2:535520/140‑1 (MQ=255)                                                           aGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAg                                                                           >  2:727431/1‑140 (MQ=255)                                                           aGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAg                                                                           >  1:222412/1‑140 (MQ=255)                                                           aGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAg                                                                           <  1:1035121/140‑1 (MQ=255)                                                             aaTTTTACTCGATTTATCGTATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAgtg                                                                         >  1:77691/1‑140 (MQ=255)                                                             aaTTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAgtg                                                                         >  2:178482/1‑140 (MQ=255)                                                                      cGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCa                                                                <  1:178482/140‑1 (MQ=255)                                                                        aTTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATc                                                              <  1:727431/140‑1 (MQ=255)                                                                         tttATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCt                                                             >  1:300656/1‑140 (MQ=255)                                                                            aTCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGc                                                          <  1:989938/140‑1 (MQ=255)                                                                             tCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCa                                                         <  1:1041588/140‑1 (MQ=255)                                                                              cGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCaa                                                        <  1:751889/140‑1 (MQ=255)                                                                              cGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCaa                                                        <  1:679038/140‑1 (MQ=255)                                                                              cGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCaa                                                        <  1:520600/140‑1 (MQ=255)                                                                              cGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCaa                                                        <  2:754132/140‑1 (MQ=255)                                                                                aaTGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAgg                                                      <  1:771630/140‑1 (MQ=255)                                                                                   gAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGgtg                                                   >  2:1041930/1‑140 (MQ=255)                                                                                    aGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGgtgt                                                  <  2:259727/140‑1 (MQ=255)                                                                                     ggTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGtgtg                                                 >  2:329260/1‑140 (MQ=255)                                                                                       tGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAg                                               >  2:155686/1‑140 (MQ=255)                                                                                         aTTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGa                                             >  1:968661/1‑140 (MQ=255)                                                                                           tAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCaa                                                        <  1:1016711/127‑1 (MQ=255)                                                                                           tAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCaa                                                        >  2:1016711/1‑127 (MQ=255)                                                                                            aaGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGa                                          <  1:1041930/140‑1 (MQ=255)                                                                                            aaGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGa                                          <  2:859589/140‑1 (MQ=255)                                                                                            aaGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGa                                          <  2:300656/140‑1 (MQ=255)                                                                                               cAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATgg                                       >  1:74266/1‑140 (MQ=255)                                                                                                aaCTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGgt                                      >  1:207271/1‑140 (MQ=255)                                                                                                 aCTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGgtg                                     <  1:155686/140‑1 (MQ=255)                                                                                                  cTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGgtgt                                    >  2:394810/1‑140 (MQ=255)                                                                                                   ttGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTc                                   <  2:1047154/140‑1 (MQ=255)                                                                                                   ttGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTc                                   >  2:410102/1‑140 (MQ=255)                                                                                                   ttGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTc                                   >  1:857633/1‑140 (MQ=255)                                                                                                   ttGATGATCTCGAGGCGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTc                                   >  2:253069/1‑140 (MQ=255)                                                                                                        gatCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAata                              <  1:923229/140‑1 (MQ=255)                                                                                                        gatCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAata                              <  2:968661/140‑1 (MQ=255)                                                                                                        gatCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAata                              <  1:253069/140‑1 (MQ=255)                                                                                                         atCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAatat                             <  2:74266/140‑1 (MQ=255)                                                                                                         atCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAatat                             >  2:60131/1‑140 (MQ=255)                                                                                                               aGGTGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGt                       <  2:939575/140‑1 (MQ=255)                                                                                                                  tGCAGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAg                    <  2:265494/140‑1 (MQ=255)                                                                                                                     aGCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTg                 >  1:789260/1‑140 (MQ=255)                                                                                                                      gCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTgc                >  2:532538/1‑140 (MQ=255)                                                                                                                      gCGTAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTgc                <  1:329260/140‑1 (MQ=255)                                                                                                                         tAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGcgcg             <  2:197220/140‑1 (MQ=255)                                                                                                                         tAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGcgcg             <  2:207271/140‑1 (MQ=255)                                                                                                                         tAATCTTCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGcgcg             <  1:532538/140‑1 (MQ=255)                                                                                                                              ttCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGcgcg             >  1:443202/1‑135 (MQ=255)                                                                                                                              ttCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGcgcg             <  2:443202/135‑1 (MQ=255)                                                                                                                              ttCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGaa        >  1:626520/1‑140 (MQ=255)                                                                                                                              ttCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGaa        <  1:638126/140‑1 (MQ=255)                                                                                                                               tCCTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAAt       <  1:394810/140‑1 (MQ=255)                                                                                                                                 cTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTgcg               >  1:443501/1‑130 (MQ=255)                                                                                                                                 cTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTgcg               >  1:285550/1‑130 (MQ=255)                                                                                                                                 cTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTgcg               <  2:443501/130‑1 (MQ=255)                                                                                                                                 cTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTgcg               >  1:986153/1‑130 (MQ=255)                                                                                                                                 cTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTgcg               <  2:986153/130‑1 (MQ=255)                                                                                                                                 cTCGTGCGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTgcg               <  2:285550/130‑1 (MQ=255)                                                                                                                                       cGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTaa  >  1:376941/1‑137 (MQ=255)                                                                                                                                       cGGGCCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTaa  <  2:376941/137‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      ATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAA  >  NC_000913/4079699‑4079969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑