Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,095,618	G→A	A122V (GCG→GTG)	rhaA ←	L‑rhamnose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,095,618	0	G	A	100.0%	204.0 / NA	58	A122V (GCG→GTG)	rhaA	L‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/32);  total (26/32)

GACGGCTGGCTTTGCAGTGATCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCGCCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGAT  >  NC_000913/4095484‑4095750                                                                                                                                       |                                                                                                                                     gACGGCTGGCTTTGCAGTGATCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCAt                                                                                                                                 >  2:385109/1‑140 (MQ=255)      ctggctTTGCAGTGATCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAAc                                                                                                                            <  1:269656/140‑1 (MQ=255)        ggctTTGCAGTGATCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAAccc                                                                                                                          <  2:325852/140‑1 (MQ=255)             tGCAGTGATCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGttt                                                                                                                     >  1:6514/1‑140 (MQ=255)                  tGATCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCTTGCTGATTTGCTTTCACCCATTCAACCCAGTTTTTGaa                                                                                                                <  2:157255/140‑1 (MQ=255)                  tGATCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGaa                                                                                                                <  2:960104/140‑1 (MQ=255)                   gATCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAg                                                                                                               >  2:992562/1‑140 (MQ=255)                   gATCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAg                                                                                                               >  1:157790/1‑140 (MQ=255)                    aTCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGt                                                                                                              >  1:470210/1‑140 (MQ=255)                     tCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTg                                                                                                             <  1:992562/140‑1 (MQ=255)                       aaTCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGct                                                                                                           <  1:185628/140‑1 (MQ=255)                       aaTCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGct                                                                                                           >  1:986279/1‑140 (MQ=255)                         tCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGctct                                                                                                         >  2:773432/1‑140 (MQ=255)                         tCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGc                                                                                                            <  2:880985/137‑1 (MQ=255)                         tCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGc                                                                                                            >  1:880985/1‑137 (MQ=255)                            aGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATC‑GCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGct                                                                                                           <  2:727193/134‑1 (MQ=255)                            aGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATC‑GCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGct                                                                                                           >  1:727193/1‑134 (MQ=255)                                 tGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGAt                                                                                                 <  2:986279/140‑1 (MQ=255)                                   gCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGAt                                                                                                 >  2:93450/1‑138 (MQ=255)                                   gCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGAt                                                                                                 <  1:93450/138‑1 (MQ=255)                                   gCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCt                                                                                               >  1:959608/1‑140 (MQ=255)                                   gCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCt                                                                                               >  2:129719/1‑140 (MQ=255)                                   gCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCt                                                                                               >  1:467831/1‑140 (MQ=255)                                   gCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCt                                                                                               >  2:409234/1‑140 (MQ=255)                                   gCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCt                                                                                               >  1:181760/1‑140 (MQ=255)                                     gAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTgg                                                                                             <  1:878063/140‑1 (MQ=255)                                        tGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTcg                                                                                          >  1:56404/1‑140 (MQ=255)                                          cTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAACTCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTcgcg                                                                                        <  2:729947/140‑1 (MQ=255)                                          cTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTcgcg                                                                                        <  2:418417/140‑1 (MQ=255)                                          cTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTcgcg                                                                                        <  2:326529/140‑1 (MQ=255)                                             tcgtcgGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCga                                                                                     >  2:690956/1‑140 (MQ=255)                                              cgtcgGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCgag                                                                                    <  2:93814/140‑1 (MQ=255)                                               gtcgGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCgaga                                                                                   <  2:467831/140‑1 (MQ=255)                                               gtcgGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCgaga                                                                                   <  2:157790/140‑1 (MQ=255)                                                  ggCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTg                                                                                >  2:491027/1‑140 (MQ=255)                                                     aTGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCga                                                                                     >  1:66658/1‑132 (MQ=255)                                                     aTGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCga                                                                                     <  2:66658/132‑1 (MQ=255)                                                     aTGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCg                                                                             <  2:894685/140‑1 (MQ=255)                                                     aTGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCg                                                                             <  2:97236/140‑1 (MQ=255)                                                         gAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATc                                                                         <  2:511636/140‑1 (MQ=255)                                                         gAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATc                                                                         <  2:56404/140‑1 (MQ=255)                                                               gTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTc                                                                   <  1:409234/140‑1 (MQ=255)                                                                tAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTcc                                                                  <  2:80227/140‑1 (MQ=255)                                                                   aGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGAt                                                                     <  1:399944/134‑1 (MQ=255)                                                                   aGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGAt                                                                     >  2:399944/1‑134 (MQ=255)                                                                   aGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCaga                                                               <  2:75755/140‑1 (MQ=255)                                                                        tCGGCGCTTAGCGGATGCGAAAAGCGGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCagatagat                                                          <  1:835110/140‑1 (MQ=255)                                                                         cGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATg                                                         <  2:372364/140‑1 (MQ=255)                                                                                 aGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTaa                                                 >  1:677527/1‑140 (MQ=255)                                                                                      aTGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTaa                                            >  2:545330/1‑140 (MQ=255)                                                                                      aTGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTaa                                            >  1:267226/1‑140 (MQ=255)                                                                                               gCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCg                                   <  1:385109/140‑1 (MQ=255)                                                                                                    aggGGTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGcccc                              <  1:491027/140‑1 (MQ=255)                                                                                                        gTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGaa                          >  1:406565/1‑140 (MQ=255)                                                                                                        gTTAAAATCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGaa                          >  2:1018528/1‑140 (MQ=255)                                                                                                           aaaaTCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCa                       <  2:134315/140‑1 (MQ=255)                                                                                                           aaaaTCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCa                       <  1:129719/140‑1 (MQ=255)                                                                                                            aaaTCCAGACCGAGCTGATTGGCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAg                      <  2:406565/140‑1 (MQ=255)                                                                                                                                ggCTTTCACCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGAt  >  1:934693/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     GACGGCTGGCTTTGCAGTGATCAATCCAGAACTGGCGAATGCTGTCGTCGGCATGGGAAAGCGTAAAGCCATCGGCGCTTAGCGGATGCGAAAAGCAGGAGGGGTTAAAATCCAGACCGAGCTGATTGGCTTTCGCCCATTCAACCCAGTTTTTGAAGTGCTCTGGTTTGATCTGGTCGCGCGAGACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGAT  >  NC_000913/4095484‑4095750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑