Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,146,746	A→G	L163L (TTA→TTG)	pflC →	putative pyruvate formate‑lyase 2 activating enzyme PflC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,146,746	0	A	G	100.0%	155.7 / NA	43	L163L (TTA→TTG)	pflC	putative pyruvate formate‑lyase 2 activating enzyme PflC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/18);  total (25/18)

GGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAAC  >  NC_000913/4146615‑4146881                                                                                                                                    |                                                                                                                                        ggcgTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGc                                                                                                                                 <  2:714408/140‑1 (MQ=255) ggcgTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGc                                                                                                                                 >  1:723058/1‑140 (MQ=255)          cTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATtgtg                                                                                                                        <  2:757710/140‑1 (MQ=255)          cTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATtgtg                                                                                                                        <  1:551157/140‑1 (MQ=255)             tCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGa                                                                                                                     >  1:349058/1‑140 (MQ=255)                     cgAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAgtgt                                                                                                             >  1:751075/1‑140 (MQ=255)                     cgAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAgtgt                                                                                                             >  1:493339/1‑140 (MQ=255)                              aaTGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGAtt                                                                                                    >  1:612442/1‑140 (MQ=255)                                    ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTaaaaa                                                                                              >  1:178065/1‑140 (MQ=255)                                    ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTaaaaa                                                                                              >  2:575883/1‑140 (MQ=255)                                      cGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAAtt                                                                                            >  1:768602/1‑140 (MQ=255)                                      cGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAAtt                                                                                            >  1:491641/1‑140 (MQ=255)                                           tttGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGa                                                                                       >  1:869748/1‑140 (MQ=255)                                             tGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGAcg                                                                                     <  2:612442/140‑1 (MQ=255)                                                  cccGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACt                                                                                <  2:768602/140‑1 (MQ=255)                                                  cccGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACt                                                                                <  2:491641/140‑1 (MQ=255)                                                  cccGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACt                                                                                >  2:137915/1‑140 (MQ=255)                                                   ccGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTc                                                                               >  2:613067/1‑140 (MQ=255)                                                    cGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCa                                                                              <  2:188793/140‑1 (MQ=255)                                                     gTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAg                                                                             >  2:689040/1‑140 (MQ=255)                                                       tttttACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGgc                                                                           <  2:493339/140‑1 (MQ=255)                                                        ttttACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGgcg                                                                          <  1:575883/140‑1 (MQ=255)                                                          ttACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGcgcg                                                                        <  1:757705/140‑1 (MQ=255)                                                            aCAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCggg                                                                      <  1:613067/140‑1 (MQ=255)                                                              aGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAt                                                                    <  2:459313/140‑1 (MQ=255)                                                                 gACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAtgtg                                                                 >  1:295044/1‑140 (MQ=255)                                                                 gACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAtgtg                                                                 >  2:360894/1‑140 (MQ=255)                                                                      cGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGa                                                                     >  2:330003/1‑131 (MQ=255)                                                                      cGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGa                                                                     <  1:330003/131‑1 (MQ=255)                                                                                   tgtCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCa                                               >  2:112551/1‑140 (MQ=255)                                                                                    gtCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCAc                                              <  1:360894/140‑1 (MQ=255)                                                                                        tGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGt                                          >  1:703512/1‑140 (MQ=255)                                                                                         gcgcCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTAATGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTg                                         <  2:390848/140‑1 (MQ=255)                                                                                               ttGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGgag                                   >  1:245110/1‑140 (MQ=255)                                                                                                       gCCGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCg                           >  1:1039483/1‑140 (MQ=255)                                                                                                        ccGGAGACGCACCAGCCAGCAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGt                          <  2:1039483/140‑1 (MQ=255)                                                                                                                       ccagcAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGtgagtgag           <  2:295044/140‑1 (MQ=255)                                                                                                                       ccagcAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGtgagtgag           <  2:723058/140‑1 (MQ=255)                                                                                                                          gcAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGc        >  1:841457/1‑140 (MQ=255)                                                                                                                           cAAGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCg       >  1:708601/1‑140 (MQ=255)                                                                                                                            aaGCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGt      >  1:238960/1‑140 (MQ=255)                                                                                                                              gCTATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCa    >  2:130376/1‑140 (MQ=255)                                                                                                                                tATTGCCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAAc  >  2:361911/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        GGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAAC  >  NC_000913/4146615‑4146881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑