Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,366,473	A→G	F101L (TTT→CTT)	dcuA ←	C4‑dicarboxylate transporter DcuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,366,473	0	A	G	100.0%	216.5 / NA	59	F101L (TTT→CTT)	dcuA	C4‑dicarboxylate transporter DcuA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/32);  total (27/32)

ATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATA  >  NC_000913/4366337‑4366606                                                                                                                                         |                                                                                                                                      aTTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGAt                                                                                                                                    <  2:192661/140‑1 (MQ=255)    ggCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGt                                                                                                                                 <  1:94911/140‑1 (MQ=255)    ggCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGt                                                                                                                                 <  1:955661/140‑1 (MQ=255)     gCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTc                                                                                                                                <  1:1035108/140‑1 (MQ=255)     gCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTc                                                                                                                                <  1:685325/140‑1 (MQ=255)          gCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGaa                                                                                                                           <  2:14740/140‑1 (MQ=255)           cGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGaaa                                                                                                                          <  1:547216/140‑1 (MQ=255)            ggTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAAt                                                                                                                         <  1:879722/140‑1 (MQ=255)                aTCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGt                                                                                                                     >  1:485983/1‑140 (MQ=255)                  cGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCa                                                                                                                   <  2:4093/140‑1 (MQ=255)                   gCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGGTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGAAGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCAc                                                                                                                  <  2:631017/140‑1 (MQ=255)                     aaTCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGAAAGTCAGGAAAAAGGTCCCGa                                                                                                                >  1:326636/1‑140 (MQ=255)                      aTCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGAt                                                                                                               <  2:427416/140‑1 (MQ=255)                      aTCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGAt                                                                                                               <  1:976923/140‑1 (MQ=255)                      aTCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGAt                                                                                                               <  1:848815/140‑1 (MQ=255)                      aTCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTACGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGAt                                                                                                               <  2:918543/140‑1 (MQ=255)                        cTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCg                                                                                                             >  1:571166/1‑140 (MQ=255)                           cgcgGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTg                                                                                                          >  1:15910/1‑140 (MQ=255)                           cgcgGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTg                                                                                                          >  1:69014/1‑140 (MQ=255)                             cgGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCg                                                                                                        <  2:571166/140‑1 (MQ=255)                             cgGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCg                                                                                                        <  2:925708/140‑1 (MQ=255)                               gATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAg                                                                                                      <  2:857899/140‑1 (MQ=255)                                   ccACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATc                                                                                                  <  2:69014/140‑1 (MQ=255)                                        gCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGAt                                                                                             <  2:15910/140‑1 (MQ=255)                                            tAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTAt                                                                                         >  1:25840/1‑140 (MQ=255)                                              gACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTAttt                                                                                       <  2:485983/140‑1 (MQ=255)                                                 aGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCgg                                                                                    >  2:443394/1‑140 (MQ=255)                                                   cGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGa                                                                                                    >  1:559890/1‑122 (MQ=255)                                                   cGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGa                                                                                                    <  2:559890/122‑1 (MQ=255)                                                   cGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGt                                                                                  >  1:224473/1‑140 (MQ=255)                                                      aCGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAc                                                                               >  2:837580/1‑140 (MQ=255)                                                                ttAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGcagcag                                                                     <  1:756907/140‑1 (MQ=255)                                                                ttAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGcagcag                                                                     <  1:837580/140‑1 (MQ=255)                                                                  aaCGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCt                                                                   >  2:499088/1‑140 (MQ=255)                                                                        ttGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTtctg                                                             >  1:38779/1‑140 (MQ=255)                                                                        ttGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTtctg                                                             >  1:751760/1‑140 (MQ=255)                                                                        ttGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTtctg                                                             >  2:482646/1‑140 (MQ=255)                                                                         tGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTtctgt                                                            >  2:759980/1‑140 (MQ=255)                                                                         tGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTtctg                                                             >  2:366456/1‑139 (MQ=255)                                                                         tGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTtctg                                                             <  1:366456/139‑1 (MQ=255)                                                                              cTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCtgat                                                       >  1:695287/1‑140 (MQ=255)                                                                                 cGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGaa                                                    <  1:443394/140‑1 (MQ=255)                                                                                 cGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGaa                                                    <  1:868832/140‑1 (MQ=255)                                                                                 cGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGaa                                                    <  1:759980/140‑1 (MQ=255)                                                                                   cAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAAcc                                                  >  1:439091/1‑140 (MQ=255)                                                                                     aCTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAg                                                >  1:423306/1‑140 (MQ=255)                                                                                     aCTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCAAGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAg                                                >  2:637910/1‑140 (MQ=255)                                                                                            cGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTcc                                         <  2:777259/140‑1 (MQ=255)                                                                                                aaCTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGAc                                     <  1:131947/140‑1 (MQ=255)                                                                                                      cAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAg                               >  1:985772/1‑140 (MQ=255)                                                                                                         tgtCGCCAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCaa                            >  1:437273/1‑140 (MQ=255)                                                                                                              ccAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGc                       <  1:482646/140‑1 (MQ=255)                                                                                                               cAGAGAGATGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCa                      <  1:499088/140‑1 (MQ=255)                                                                                                                       tGTTGCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCAAGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaa              <  1:637910/140‑1 (MQ=255)                                                                                                                           gCCAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAg          >  1:40218/1‑140 (MQ=255)                                                                                                                            ccAGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAgc         >  1:787092/1‑140 (MQ=255)                                                                                                                              aGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAgcgg       >  1:936792/1‑140 (MQ=255)                                                                                                                              aGTACCAGCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAgcgg       >  1:789435/1‑140 (MQ=255)                                                                                                                                   cAGCAAGGATAGCCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATa  >  2:776359/1‑140 (MQ=255)                                                                                                                                     gCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAg          >  2:336504/1‑130 (MQ=255)                                                                                                                                     gCAAGGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAg          <  1:336504/130‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      ATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATA  >  NC_000913/4366337‑4366606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑