Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	502,665	T→C	T192A (ACC→GCC)	ybaL ←	putative transporter YbaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	502,665	0	T	C	100.0%	187.8 / NA	52	T192A (ACC→GCC)	ybaL	putative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/25);  total (27/25)

AACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCA  >  NC_000913/502532‑502801                                                                                                                                      |                                                                                                                                         aaCGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATccc                                                                                                                                    >  1:553702/1‑140 (MQ=255)       agcgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATc                                                                                                                              >  1:727092/1‑140 (MQ=255)       agcgccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATc                                                                                                                              >  1:430194/1‑140 (MQ=255)          gccagcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGAc                                                                                                                           <  1:28170/140‑1 (MQ=255)              gcACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCa                                                                                                                       <  2:430194/140‑1 (MQ=255)                  cGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAagag                                                                                                                   <  2:535022/140‑1 (MQ=255)                      aGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGc                                                                                                               <  1:20037/140‑1 (MQ=255)                         gTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCa                                                                                                              <  1:360525/138‑1 (MQ=255)                         gTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCa                                                                                                              >  2:360525/1‑138 (MQ=255)                           aaaCAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGc                                                                                                          <  2:284178/140‑1 (MQ=255)                            aaCAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGcc                                                                                                         >  2:176552/1‑140 (MQ=255)                             aCAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGccc                                                                                                        <  1:930826/140‑1 (MQ=255)                             aCAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGccc                                                                                                        >  1:829484/1‑140 (MQ=255)                                  tCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCTGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATc                                                                                                   >  1:192646/1‑140 (MQ=255)                                  tCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATc                                                                                                   >  1:306662/1‑140 (MQ=255)                                  tCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATc                                                                                                   >  2:183677/1‑140 (MQ=255)                                  tCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATc                                                                                                   >  2:1042519/1‑140 (MQ=255)                                   cgcgAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCg                                                                                                  >  2:552334/1‑140 (MQ=255)                                   cgcgAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCg                                                                                                  >  2:28666/1‑140 (MQ=255)                                    gcgAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGc                                                                                                 >  1:490093/1‑140 (MQ=255)                                        gaACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTg                                                                                             <  1:183677/140‑1 (MQ=255)                                        gaACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTg                                                                                             <  1:176552/140‑1 (MQ=255)                                             ggTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCca                                                                                        <  1:144341/140‑1 (MQ=255)                                             ggTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCca                                                                                        <  2:1020428/140‑1 (MQ=255)                                                   cgcgCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCg                                                                                                  >  1:721989/1‑124 (MQ=255)                                                   cgcgCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCCATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCg                                                                                                  <  2:721989/124‑1 (MQ=255)                                                                 tAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGgc                                                                    <  2:727092/140‑1 (MQ=255)                                                                    tCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCgg                                                                      >  1:156761/1‑135 (MQ=255)                                                                    tCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCgg                                                                      <  2:156761/135‑1 (MQ=255)                                                                         ggCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAacac                                                            >  2:626699/1‑140 (MQ=255)                                                                                gACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCgg                                                                      <  2:1006599/123‑1 (MQ=255)                                                                                gACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCgg                                                                      >  1:1006599/1‑123 (MQ=255)                                                                                           aCCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc                                                >  2:825007/1‑134 (MQ=255)                                                                                           aCCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAAc                                                <  1:825007/134‑1 (MQ=255)                                                                                           aCCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCAtt                                            <  1:573140/138‑1 (MQ=255)                                                                                           aCCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCAtt                                            >  2:573140/1‑138 (MQ=255)                                                                                            ccAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTAcc                                         <  2:239940/140‑1 (MQ=255)                                                                                            acAGCATCATAATGACGAAAAATGCGATCACTTGGGAGATGACGATCCCTAATTCGACTGAAAGAGTGGCAAAGCCCAGATAGCACTGTACAATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTAcc                                         <  2:821538/139‑1 (MQ=255)                                                                                               gCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAgg                                      <  1:479713/140‑1 (MQ=255)                                                                                                    atAATGGCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTc                                 >  1:840668/1‑140 (MQ=255)                                                                                                          gCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGTAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCca                               >  2:562281/1‑136 (MQ=255)                                                                                                          gCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCca                               <  1:562281/136‑1 (MQ=255)                                                                                                          gCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAAt                           >  1:448632/1‑140 (MQ=255)                                                                                                          gCGATAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAAt                           >  2:465658/1‑140 (MQ=255)                                                                                                             aTAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaa                        >  1:830012/1‑140 (MQ=255)                                                                                                             aTAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaa                        >  1:538315/1‑140 (MQ=255)                                                                                                              tAAATGCGATCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATcaac                       <  2:538315/140‑1 (MQ=255)                                                                                                                       tCACTTTGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATg              <  1:626699/140‑1 (MQ=255)                                                                                                                           tttGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGa          <  1:1042519/140‑1 (MQ=255)                                                                                                                             tGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGTGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGAt         >  2:39745/1‑139 (MQ=255)                                                                                                                             tGCCGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGTGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGAt         <  1:39745/139‑1 (MQ=255)                                                                                                                               ccGATGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTg      <  1:140778/140‑1 (MQ=255)                                                                                                                                   tGGCGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGccac  >  1:603270/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                                         AACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTCCATCATTCCTGCCACTGCGGGCAGCAACACCAGCGTCAGAACCATTACCAGGTCTTCCACAATCAACCAACCGATGGCGATTTGCCCA  >  NC_000913/502532‑502801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑