Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,533,547	G→A	H80Y (CAC→TAC)	yjhQ ←	KpLE2 phage‑like element; putative acetyltransferase TopAI antitoxin YjhQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,533,547	0	G	A	100.0%	282.4 / NA	79	H80Y (CAC→TAC)	yjhQ	KpLE2 phage‑like element; putative acetyltransferase TopAI antitoxin YjhQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/39);  total (40/39)

GTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAA  >  NC_000913/4533410‑4533682                                                                                                                                          |                                                                                                                                        gTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACa                                                                                                                                       >  2:815790/1‑140 (MQ=255)  tAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACAt                                                                                                                                      <  1:815790/140‑1 (MQ=255)         tggcgtggcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGt                                                                                                                               >  1:530985/1‑140 (MQ=255)         tggcgtggcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGt                                                                                                                               >  1:216995/1‑140 (MQ=255)         tggcgtggcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGt                                                                                                                               >  1:228769/1‑140 (MQ=255)          ggcgtggcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTg                                                                                                                              >  1:592806/1‑140 (MQ=255)           gcgtggcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGa                                                                                                                             <  1:695029/140‑1 (MQ=255)            cgtggcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGaa                                                                                                                            >  1:329546/1‑140 (MQ=255)            cgtggcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGaa                                                                                                                            >  1:883145/1‑140 (MQ=255)              tggcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGa                                                                                                                             <  2:462682/137‑1 (MQ=255)              tggcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGa                                                                                                                             >  1:462682/1‑137 (MQ=255)                gcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCa                                                                                                                        >  1:305680/1‑140 (MQ=255)                gcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCa                                                                                                                        >  2:125065/1‑140 (MQ=255)                     gaACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTtct                                                                                                                   <  2:513970/140‑1 (MQ=255)                     gaACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTtct                                                                                                                   <  1:206920/140‑1 (MQ=255)                     gaACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTtct                                                                                                                   <  2:329546/140‑1 (MQ=255)                     gaACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTtct                                                                                                                   <  2:323976/140‑1 (MQ=255)                     gaACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGGATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTtct                                                                                                                   >  1:756400/1‑140 (MQ=255)                         aaaaaCCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCtt                                                                                                               <  2:348643/140‑1 (MQ=255)                          aaaaCCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCttt                                                                                                              <  1:968355/140‑1 (MQ=255)                           aaaCCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTg                                                                                                             >  1:261884/1‑140 (MQ=255)                                  cTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTg                                                                                                      >  1:887823/1‑140 (MQ=255)                                    ggCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGcc                                                                                                    <  2:609274/140‑1 (MQ=255)                                       aGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCgg                                                                                                 <  2:912846/140‑1 (MQ=255)                                            aTCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCgg                                                                                                 <  2:1395/135‑1 (MQ=255)                                            aTCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCgg                                                                                                 >  1:1395/1‑135 (MQ=255)                                            aTCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGtaaa                                                                                            <  2:607821/140‑1 (MQ=255)                                            aTCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGtaaa                                                                                            <  2:305680/140‑1 (MQ=255)                                                       gTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGcc                                                                                 >  2:403371/1‑140 (MQ=255)                                                         gCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCg                                                                               >  1:208673/1‑140 (MQ=255)                                                          cTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGa                                                                              >  1:386404/1‑140 (MQ=255)                                                               tGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCt                                                                         >  1:760644/1‑140 (MQ=255)                                                                gCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCtt                                                                        <  2:228769/140‑1 (MQ=255)                                                                     tGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCtt                                                                        >  1:939410/1‑135 (MQ=255)                                                                     tGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGCGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCtt                                                                        <  2:939410/135‑1 (MQ=255)                                                                         tATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCAt                                                               >  1:354414/1‑140 (MQ=255)                                                                         tATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCAt                                                               <  2:883145/140‑1 (MQ=255)                                                                               ccGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCAt                                                               <  1:313245/134‑1 (MQ=255)                                                                               ccGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCAt                                                               >  2:313245/1‑134 (MQ=255)                                                                               ccGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCggg                                                         >  2:846583/1‑140 (MQ=255)                                                                                  tcctccCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTa                                                      >  2:1032073/1‑140 (MQ=255)                                                                                   cctccCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTag                                                     <  2:386404/140‑1 (MQ=255)                                                                                    ctccCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTagc                                                    >  1:167886/1‑140 (MQ=255)                                                                                          cGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGAt                                              <  2:760644/140‑1 (MQ=255)                                                                                               aTCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAAcgc                                         >  2:360511/1‑140 (MQ=255)                                                                                                tCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAAcgcg                                        >  1:931184/1‑140 (MQ=255)                                                                                                 ctcTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCgg                                       >  2:767022/3‑140 (MQ=255)                                                                                                 cccTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCgg                                       >  1:157730/1‑140 (MQ=255)                                                                                                     tGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGgcg                                   >  2:862705/1‑140 (MQ=255)                                                                                                       gTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACGTCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACCGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGgcgcg                                 <  2:439442/140‑1 (MQ=255)                                                                                                       gTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGgcgcg                                 <  1:360511/140‑1 (MQ=255)                                                                                                         aTTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGgcgcgcg                               <  1:403371/140‑1 (MQ=255)                                                                                                         aTTCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGgcgcgcg                               <  1:967549/140‑1 (MQ=255)                                                                                                           tCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGgcgc                                  >  2:825394/1‑135 (MQ=255)                                                                                                           tCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGgcgc                                  <  1:825394/135‑1 (MQ=255)                                                                                                           tCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCt                             >  2:355998/1‑140 (MQ=255)                                                                                                           tCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCt                             >  1:696388/1‑140 (MQ=255)                                                                                                           tCCGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCt                             >  1:784868/1‑140 (MQ=255)                                                                                                             cGGTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCttt                           >  2:233306/1‑140 (MQ=255)                                                                                                              ggTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTc                          >  1:913640/1‑140 (MQ=255)                                                                                                               gTATGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCa                         <  2:991138/140‑1 (MQ=255)                                                                                                                 aTGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATc                       <  2:216995/140‑1 (MQ=255)                                                                                                                 aTGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATc                       <  2:354414/140‑1 (MQ=255)                                                                                                                 aTGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATc                       <  2:167886/140‑1 (MQ=255)                                                                                                                  tGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATcc                      <  1:767022/140‑1 (MQ=255)                                                                                                                  tGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATcc                      >  1:561597/1‑140 (MQ=255)                                                                                                                  tGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATc                       <  1:673692/139‑1 (MQ=255)                                                                                                                  tGACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATc                       >  2:673692/1‑139 (MQ=255)                                                                                                                   gACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCt                     <  2:794546/140‑1 (MQ=255)                                                                                                                   gACCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCt                     <  1:196883/140‑1 (MQ=255)                                                                                                                    aCCGCCAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTc                    <  2:913640/140‑1 (MQ=255)                                                                                                                         cAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCa                         <  2:806340/130‑1 (MQ=255)                                                                                                                         cAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCa                         >  1:806340/1‑130 (MQ=255)                                                                                                                         cAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTa               <  2:756400/140‑1 (MQ=255)                                                                                                                         cAGGGGAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTa               >  2:603442/1‑140 (MQ=255)                                                                                                                           ggggAGCGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTaaa             <  2:208673/140‑1 (MQ=255)                                                                                                                                 cGAGGATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGcc       <  2:887823/140‑1 (MQ=255)                                                                                                                                    ggATGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACt    <  1:355998/140‑1 (MQ=255)                                                                                                                                      aTGTACATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTaa  >  2:30995/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCTCAAGTAAAGAGGCCACTAA  >  NC_000913/4533410‑4533682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑