Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	571,064	T→C	S58P (TCT→CCT)	ybcL →	DLP12 prophage; periplasmic protein YbcL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	571,064	0	T	C	100.0%	305.6 / NA	83	S58P (TCT→CCT)	ybcL	DLP12 prophage; periplasmic protein YbcL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/51);  total (32/51)

CATAACATTTTCTGCGCAGGCTGCAGCATTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGTCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCGTTGATGCAGGG  >  NC_000913/570928‑571198                                                                                                                                         |                                                                                                                                       cATAACATTTTCTGCGCAGGCTGCAGCATTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACctggcctg                                                                                                                                     >  2:859736/1‑140 (MQ=255) cATAACATTTTCTGCGCAGGCTGCAGCATTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACctggcctg                                                                                                                                     >  1:169821/1‑140 (MQ=255) cATAACATTTTCTGCGCAGGCTGCAGCATTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACctggcctg                                                                                                                                     >  1:303952/1‑140 (MQ=255)    aaCATTTTCTGCGCAGGCTGCAGCATTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGgtg                                                                                                                                  <  1:76061/140‑1 (MQ=255)           tCTGCGCAGGCTGCAGCATTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGa                                                                                                                           >  2:643896/1‑140 (MQ=255)                gcAGGCTGCAGCATTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTa                                                                                                                      >  2:467499/1‑140 (MQ=255)                    gCTGCAGCATTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGaa                                                                                                                          >  1:723934/1‑132 (MQ=255)                    gCTGCAGCATTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGaa                                                                                                                          <  2:723934/132‑1 (MQ=255)                        cagcaTTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGc                                                                                                              <  1:853986/140‑1 (MQ=255)                        cagcaTTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGc                                                                                                              <  2:303952/140‑1 (MQ=255)                         agcaTTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCt                                                                                                             <  2:1001469/140‑1 (MQ=255)                          gcaTTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCtt                                                                                                            <  2:62377/140‑1 (MQ=255)                             tttCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGc                                                                                                         <  1:208690/140‑1 (MQ=255)                              ttCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGcc                                                                                                        >  2:356596/1‑140 (MQ=255)                               tCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCg                                                                                                       <  1:937193/140‑1 (MQ=255)                               tCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCg                                                                                                       <  2:685075/140‑1 (MQ=255)                               tCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCg                                                                                                       <  1:248109/140‑1 (MQ=255)                               tCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGAGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCg                                                                                                       <  2:729936/140‑1 (MQ=255)                                cAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGt                                                                                                      <  1:356596/140‑1 (MQ=255)                                cAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGt                                                                                                      <  1:597370/140‑1 (MQ=255)                                cAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGt                                                                                                      <  2:833948/140‑1 (MQ=255)                                  ggTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTaa                                                                                                    <  2:741418/140‑1 (MQ=255)                                  ggTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTaa                                                                                                    <  2:744746/140‑1 (MQ=255)                                    tCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACt                                                                                                  <  2:267751/140‑1 (MQ=255)                                        tAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTa                                                                                                     >  2:426474/1‑133 (MQ=255)                                        tAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTa                                                                                                     <  1:426474/133‑1 (MQ=255)                                        tAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGtat                                                                                              >  2:96150/1‑140 (MQ=255)                                        tAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGtat                                                                                              >  2:704619/1‑140 (MQ=255)                                         aGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGtata                                                                                             <  1:103453/140‑1 (MQ=255)                                         aGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGtata                                                                                             <  1:96150/140‑1 (MQ=255)                                           tAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATg                                                                                           >  2:363311/1‑140 (MQ=255)                                            aaTGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGa                                                                                          <  2:417252/140‑1 (MQ=255)                                             aTGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTa                                                                                                     >  2:627878/1‑128 (MQ=255)                                             aTGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTa                                                                                                     <  1:627878/128‑1 (MQ=255)                                             aTGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGAt                                                                                         <  1:363311/140‑1 (MQ=255)                                               gAAATAAAAACAGGAGAGCAACTTACAACGCCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATcc                                                                                       <  1:234966/140‑1 (MQ=255)                                                aaaTAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCa                                                                                      >  1:132709/1‑140 (MQ=255)                                                 aaTAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAg                                                                                     <  1:1005683/140‑1 (MQ=255)                                                  aTAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTaa                                                                                                    <  1:761950/124‑1 (MQ=255)                                                  aTAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTaa                                                                                                    >  2:761950/1‑124 (MQ=255)                                                  aTAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGa                                                                                    <  1:859736/140‑1 (MQ=255)                                                  aTAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGa                                                                                    <  2:149346/140‑1 (MQ=255)                                                  aTAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGa                                                                                    >  1:527552/1‑140 (MQ=255)                                                    aaaaaCAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATg                                                                                  <  1:409954/140‑1 (MQ=255)                                                    aaaaaCAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATg                                                                                  <  1:842846/140‑1 (MQ=255)                                                    aaaaaCAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATg                                                                                  <  2:169821/140‑1 (MQ=255)                                                     aaaaCAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGc                                                                                 >  2:414706/1‑140 (MQ=255)                                                     aaaaCAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGc                                                                                 >  2:160519/1‑140 (MQ=255)                                                      aaaCAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCa                                                                                <  2:513243/140‑1 (MQ=255)                                                               agCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGc                                                                       <  2:527552/140‑1 (MQ=255)                                                                 cAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAg                                                                     <  1:643896/140‑1 (MQ=255)                                                                      tACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATg                                                                                  <  2:1000994/122‑1 (MQ=255)                                                                      tACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATg                                                                                  >  1:1000994/1‑122 (MQ=255)                                                                      tACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGtggtt                                                                >  1:307533/1‑140 (MQ=255)                                                                             tctcATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCa                                                         >  1:453813/1‑140 (MQ=255)                                                                              ctcATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCAt                                                        >  2:699634/1‑140 (MQ=255)                                                                              ctcATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCAt                                                        >  1:665312/1‑140 (MQ=255)                                                                               tcATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCAtt                                                       >  1:894549/1‑140 (MQ=255)                                                                                 aTGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTgg                                                     >  1:764772/1‑140 (MQ=255)                                                                                  tGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGa                                                    <  2:309946/140‑1 (MQ=255)                                                                                  tGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGa                                                    <  2:307533/140‑1 (MQ=255)                                                                                  tGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGa                                                    <  2:1030298/140‑1 (MQ=255)                                                                                  tGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGa                                                    <  1:467499/140‑1 (MQ=255)                                                                                       tttCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACtgtt                                               >  1:146143/1‑140 (MQ=255)                                                                                        ttCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACtgttg                                              >  2:139230/1‑140 (MQ=255)                                                                                        ttCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACtgttg                                              >  1:209663/1‑140 (MQ=255)                                                                                           tGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTa                                           <  1:160519/140‑1 (MQ=255)                                                                                           tGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTa                                           <  1:414706/140‑1 (MQ=255)                                                                                                     tgtgAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGc                                 <  1:483861/140‑1 (MQ=255)                                                                                                     tgtgAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGc                                 <  2:894549/140‑1 (MQ=255)                                                                                                       tgAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACtgt                                                <  2:918916/123‑1 (MQ=255)                                                                                                       tgAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACtgt                                                >  1:918916/1‑123 (MQ=255)                                                                                                       tgAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCaa                               <  1:858911/140‑1 (MQ=255)                                                                                                       tgAAGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCaa                               >  2:983150/1‑140 (MQ=255)                                                                                                         aaGGTGGTAATACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACa                             <  1:699634/140‑1 (MQ=255)                                                                                                                 aaTACATCGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACata                     >  1:95054/1‑140 (MQ=255)                                                                                                                        cGCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGccc              >  1:392607/1‑140 (MQ=255)                                                                                                                         gCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCg             <  1:314680/140‑1 (MQ=255)                                                                                                                         gCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCg             >  2:446737/1‑140 (MQ=255)                                                                                                                         gCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCg             <  2:392607/140‑1 (MQ=255)                                                                                                                         gCCCTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCg             >  2:314680/1‑140 (MQ=255)                                                                                                                          cccTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCGt            <  2:764772/140‑1 (MQ=255)                                                                                                                          cccTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCGt            >  2:979902/1‑140 (MQ=255)                                                                                                                          cccTCATTAACCTGGCCTGGCGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGAATATGATCCAGATGCACCTACAGGCAGCGGTTGGAGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCGt            >  2:64701/1‑140 (MQ=255)                                                                                                                            cTCATTAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCGTTg          <  1:836386/140‑1 (MQ=255)                                                                                                                                 tAACCTGGCCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCGTTGATGCa     <  2:132709/140‑1 (MQ=255)                                                                                                                                    cctggcctggTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCGTTGATGCAggg  >  1:301370/1‑140 (MQ=255)                                                                                                                                    cctggcctggTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCGTTGATGCAggg  >  1:502006/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CATAACATTTTCTGCGCAGGCTGCAGCATTTCAGGTCACTAGTAATGAAATAAAAACAGGAGAGCAACTTACAACGTCTCATGTCTTTTCTGGATTTGGGTGTGAAGGTGGTAATACATCGCCCTCATTAACCTGGTCTGGTGTTCCTGAAGGTACCAAAAGCTTTGCCGTAACTGTATATGATCCAGATGCACCTACAGGCAGTGGTTGGTGGCATTGGACTGTTGTTAATATTCCAGCAACAGTAACATATTTGCCCGTTGATGCAGGG  >  NC_000913/570928‑571198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑