| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 601,908 | A→G | intergenic (+51/‑51) | cusA → / → pheP | copper/silver export system RND permease/phenylalanine:H(+) symporter PheP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 601,908 | 0 | A | G | 98.2% | 187.4 / ‑4.3 | 54 | intergenic (+51/‑51) | cusA/pheP | copper/silver export system RND permease/phenylalanine:H(+) symporter PheP |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (25/28); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.65e-01 | |||||||||||
GATCACCGCACCTTTGCTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCAT > NC_000913/601773‑602039 | gATCACCGCACCTTTGCTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTaa < 2:840135/140‑1 (MQ=255)gATCACCGCACCTTTGCTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTaa < 1:505658/140‑1 (MQ=255)gATCACCGCACCTTTGCTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTaa < 1:825970/140‑1 (MQ=255) gCACCTTTGCTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTaa > 2:282307/1‑140 (MQ=255) cTTTGCTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGtt < 2:830768/134‑1 (MQ=255) cTTTGCTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGtt > 1:830768/1‑134 (MQ=255) ttGCTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTg > 1:691923/1‑140 (MQ=255) ttGCTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTg < 1:414772/140‑1 (MQ=255) cTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGccc > 1:145689/1‑140 (MQ=255) tttATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACGCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACaaa > 2:729636/1‑140 (MQ=255) tatCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGa < 1:895559/140‑1 (MQ=255) tatCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGa < 1:473838/140‑1 (MQ=255) tatCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGa < 1:611284/140‑1 (MQ=255) tatCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGa < 1:282307/140‑1 (MQ=255) tCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGaca < 1:598041/140‑1 (MQ=255) ccGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGacaca < 1:1031994/140‑1 (MQ=255) cggcggCGAATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGacacac > 2:541607/1‑140 (MQ=255) tgtgGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGaaaa > 2:893237/1‑140 (MQ=255) gCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAAcgc > 1:51504/1‑140 (MQ=255) aCCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCaa > 2:283096/1‑140 (MQ=255) aCCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCaa < 1:729636/140‑1 (MQ=255) gACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCg < 2:691923/140‑1 (MQ=255) gACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCg < 2:51504/140‑1 (MQ=255) aCATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGTAAGGCGTGAAAAACGCGTCAACCGt > 2:283278/1‑140 (MQ=255) cATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTa < 1:1008962/140‑1 (MQ=255) cATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTa < 1:273905/140‑1 (MQ=255) ggTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGa > 1:44458/1‑140 (MQ=255) tACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGa < 2:86292/138‑1 (MQ=255) tACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGa > 1:86292/1‑138 (MQ=255) cGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGAt > 1:848711/1‑140 (MQ=255) aaaTAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGTAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACt < 1:283278/140‑1 (MQ=255) tAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCg > 1:1030725/1‑140 (MQ=255) gATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCaa > 2:668151/1‑140 (MQ=255) ccccGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGc < 2:1030725/140‑1 (MQ=255) ttAACCGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGc > 1:587032/1‑140 (MQ=255) ttAACCGTGTGGATTGGGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGACGgcgc > 2:51001/1‑140 (MQ=255) ccGTGTGGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCa > 2:78536/1‑140 (MQ=255) ccGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCa > 1:412862/1‑140 (MQ=255) gtgGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATcgc > 2:448806/1‑140 (MQ=255) tgGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATcgcg > 2:1007003/1‑140 (MQ=255) tgGATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATcgcg < 1:78536/140‑1 (MQ=255) ggATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCgg < 2:145689/140‑1 (MQ=255) gATTGTGTCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGa < 1:541607/140‑1 (MQ=255) tgtCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACa > 1:1015337/1‑140 (MQ=255) tCTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATa > 1:627418/1‑140 (MQ=255) cTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATag < 2:587032/140‑2 (MQ=255) cTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATaa < 2:848711/140‑1 (MQ=255) cTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATaa < 1:1007003/140‑1 (MQ=255) cTTGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATaa < 1:448806/140‑1 (MQ=255) tGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAAcc > 2:741250/1‑140 (MQ=255) tGCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAAcc > 1:1009525/1‑140 (MQ=255) gCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCg > 2:180563/1‑140 (MQ=255) gCGACGATGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCg < 1:668151/140‑1 (MQ=255) gacgaTGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTc < 2:1009525/140‑1 (MQ=255) gacgaTGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTc < 1:741250/140‑1 (MQ=255) cgaTGGGCGCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCat > 1:409766/1‑140 (MQ=255) | GATCACCGCACCTTTGCTGTCGCTGTTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCAT > NC_000913/601773‑602039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |