Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	666,790	A→G	G476G (GGT→GGC)	mrdA ←	peptidoglycan DD‑transpeptidase MrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	666,790	0	A	G	100.0%	254.7 / NA	71	G476G (GGT→GGC)	mrdA	peptidoglycan DD‑transpeptidase MrdA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/36);  total (35/36)

CACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGC  >  NC_000913/666654‑666923                                                                                                                                         |                                                                                                                                      cacCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCAATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCg                                                                                                                                    >  1:707969/1‑140 (MQ=255)  acCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGt                                                                                                                                   >  2:878560/1‑140 (MQ=255)      tACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGGACCTTCGCGAAGTCGTCAtt                                                                                                                               >  2:1010023/1‑140 (MQ=255)      tACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCa                                                                                                                                 <  1:357041/138‑1 (MQ=255)      tACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCa                                                                                                                                 >  2:357041/1‑138 (MQ=255)      tACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCAtt                                                                                                                               >  1:343/1‑140 (MQ=255)      tACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCAtt                                                                                                                               >  2:8378/1‑140 (MQ=255)        cATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTaa                                                                                                                             <  1:878560/140‑1 (MQ=255)           aCCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCa                                                                                                                                 >  2:553430/1‑133 (MQ=255)           aCCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCa                                                                                                                                 <  1:553430/133‑1 (MQ=255)           aCCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCa                                                                                                                          >  2:133386/1‑140 (MQ=255)             cGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAgg                                                                                                                        <  1:875497/140‑1 (MQ=255)             cGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAgg                                                                                                                        >  2:875497/1‑140 (MQ=255)              gTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGa                                                                                                                       >  2:1043770/1‑140 (MQ=255)               tCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGat                                                                                                                      >  2:945251/1‑140 (MQ=255)                 tttCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGatca                                                                                                                    >  2:457531/1‑140 (MQ=255)                 tttCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGatca                                                                                                                    >  1:965046/1‑140 (MQ=255)                  ttCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGatcat                                                                                                                   <  1:554958/140‑1 (MQ=255)                  ttCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGatcat                                                                                                                   <  1:259073/140‑1 (MQ=255)                  ttCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGatcat                                                                                                                   <  2:977757/140‑1 (MQ=255)                   tCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGatcatc                                                                                                                  <  2:830554/140‑1 (MQ=255)                    cGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGAtcatca                                                                                                                 <  2:622739/140‑1 (MQ=255)                    cGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGAtcatca                                                                                                                 <  2:683674/140‑1 (MQ=255)                    cGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGAtcatca                                                                                                                 <  2:886514/140‑1 (MQ=255)                    cGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGAtcatca                                                                                                                 >  1:819650/1‑140 (MQ=255)                    cGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGAtcatca                                                                                                                 <  1:1043770/140‑1 (MQ=255)                    cGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCAATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGAtcatca                                                                                                                 <  2:707969/140‑1 (MQ=255)                       cAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTg                                                                                                              <  1:785678/140‑1 (MQ=255)                         gCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGAtcatca                                                                                                                 <  2:584110/135‑1 (MQ=255)                         gCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGAtcatca                                                                                                                 >  1:584110/1‑135 (MQ=255)                            cccAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTa                                                                                                         <  2:932589/140‑1 (MQ=255)                               aGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGAtcatca                                                                                                                 >  2:597884/1‑129 (MQ=255)                               aGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGAtcatca                                                                                                                 <  1:597884/129‑1 (MQ=255)                                 tAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCAt                                                                                                    <  1:901532/140‑1 (MQ=255)                                    ccGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTg                                                                                                 >  1:677548/1‑140 (MQ=255)                                            aatATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTgggg                                                                                         <  2:863393/140‑1 (MQ=255)                                               atCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCg                                                                                      >  1:342547/1‑140 (MQ=255)                                               atCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCg                                                                                      >  1:360795/1‑140 (MQ=255)                                               atCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCg                                                                                      <  2:360795/140‑1 (MQ=255)                                               atCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCg                                                                                      >  1:455495/1‑140 (MQ=255)                                               atCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCg                                                                                      <  1:8378/140‑1 (MQ=255)                                                  gCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTg                                                                                   >  1:686969/1‑140 (MQ=255)                                                  gCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTg                                                                                   <  1:376722/140‑1 (MQ=255)                                                         ggCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTa                                                                            >  2:231596/1‑140 (MQ=255)                                                            ggTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAAcc                                                                         <  2:819650/140‑1 (MQ=255)                                                            ggTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAAcc                                                                         <  2:455495/140‑1 (MQ=255)                                                              ttCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCt                                                                       <  1:945251/140‑1 (MQ=255)                                                                 aTGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGAc                                                                    <  1:133386/140‑1 (MQ=255)                                                                        tGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATAcc                                                             <  2:921766/140‑1 (MQ=255)                                                                        tGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACCATGCCGTCATTAATAAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATAcc                                                             >  1:89063/1‑140 (MQ=255)                                                                          tACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCaa                                                           >  1:114285/1‑140 (MQ=255)                                                                            cccATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAAcc                                                         >  1:413819/1‑140 (MQ=255)                                                                                tGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGaa                                                     <  2:114285/140‑1 (MQ=255)                                                                                    aCCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGgt                                                 <  2:343/140‑1 (MQ=255)                                                                                    aCCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGgt                                                 >  2:737450/1‑140 (MQ=255)                                                                                      cTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGgtgt                                               <  1:1010023/140‑1 (MQ=255)                                                                                      cTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGgtgt                                               <  2:123509/140‑1 (MQ=255)                                                                                      cTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGCCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGgtgt                                               >  2:382420/1‑140 (MQ=255)                                                                                                 cTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATAc                                    >  2:90394/1‑140 (MQ=255)                                                                                                      gcggTGCTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACgg                               <  2:758571/140‑1 (MQ=255)                                                                                                            cTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGtttttt                         >  2:35447/1‑140 (MQ=255)                                                                                                            cTCATCAGCAAATGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGtttttt                         >  2:988888/1‑140 (MQ=255)                                                                                                                     aaaTGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGttt                <  2:190425/140‑1 (MQ=255)                                                                                                                     aaaTGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGttt                <  2:965046/140‑1 (MQ=255)                                                                                                                      aaTGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTc               >  1:626692/1‑140 (MQ=255)                                                                                                                      aaTGAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTc               >  1:1009259/1‑140 (MQ=255)                                                                                                                         gAGGAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGt            >  1:97903/1‑140 (MQ=255)                                                                                                                            gAACCTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTc         <  2:342547/140‑1 (MQ=255)                                                                                                                               ccTTCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCAt      >  1:719200/1‑140 (MQ=255)                                                                                                                                 ttCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTc    >  1:52215/1‑140 (MQ=255)                                                                                                                                  tCACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTcg   <  2:413819/140‑1 (MQ=255)                                                                                                                                   cACGATGCCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTcgc  >  2:678581/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGC  >  NC_000913/666654‑666923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑