Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	813,081	C→T	intergenic (+134/‑445)	bioD → / → uvrB	dethiobiotin synthetase/excision nuclease subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	813,081	0	C	T	100.0%	121.4 / NA	36	intergenic (+134/‑445)	bioD/uvrB	dethiobiotin synthetase/excision nuclease subunit B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/24);  total (12/24)

AGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGCACGCGAATTGGCGTTAAAGACGGCTCAA  >  NC_000913/812946‑813211                                                                                                                                        |                                                                                                                                   aGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGgtggcg                                                                                                                                <  2:348138/140‑1 (MQ=255)       tCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGAt                                                                                                                          <  1:682066/140‑1 (MQ=255)       tCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGAt                                                                                                                          <  2:477655/140‑1 (MQ=255)       tCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGAt                                                                                                                          >  2:557106/1‑140 (MQ=255)       tCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGAt                                                                                                                          <  1:591610/140‑1 (MQ=255)       tCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGAt                                                                                                                          <  2:826040/140‑1 (MQ=255)       tCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGAt                                                                                                                          >  1:368190/1‑140 (MQ=255)         tGTATTTGGTTAAATTGCGAGCGAGATCGGGTTTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTCCTCCCGCCTCAGGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAg                                                                                                                        <  2:229087/140‑1 (MQ=255)         tGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAg                                                                                                                        <  2:46704/140‑1 (MQ=255)         tGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAg                                                                                                                        <  1:493445/140‑1 (MQ=255)         tGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAg                                                                                                                        <  2:53721/140‑1 (MQ=255)         tGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAg                                                                                                                        <  2:368190/140‑1 (MQ=255)                ggTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAACTAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAgggg                                                                                                                     <  2:648092/136‑1 (MQ=255)                ggTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAgggg                                                                                                                     >  1:648092/1‑136 (MQ=255)                ggTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTgg                                                                                                                 >  1:57356/1‑140 (MQ=255)                 gTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGga                                                                                                                <  2:57356/140‑1 (MQ=255)                   tAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGgaga                                                                                                              <  1:557106/140‑1 (MQ=255)                        tGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGtt                                                                                                         <  1:267192/140‑1 (MQ=255)                            agcgagATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATcc                                                                                                     >  1:321994/1‑140 (MQ=255)                                    cgcgTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATCCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTcc                                                                                             >  1:90196/1‑140 (MQ=255)                                          ttCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGa                                                                                       <  1:280762/140‑1 (MQ=255)                                            cGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACG‑AAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTgg                                                                                        >  1:781620/1‑136 (MQ=255)                                            cGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACG‑AAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTgg                                                                                        <  2:781620/136‑1 (MQ=255)                                                 gACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCAt                                                                                <  2:277059/140‑1 (MQ=255)                                                 gACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCAt                                                                                <  2:745922/140‑1 (MQ=255)                                                       aaTTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTAt                                                                          <  2:90196/140‑1 (MQ=255)                                                              ccAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATATcc                                                                                  <  2:492319/125‑1 (MQ=255)                                                              ccAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAAcc                                                                                  >  1:492319/1‑125 (MQ=255)                                                                 aTTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGa                                                                <  2:184356/140‑1 (MQ=255)                                                                             aataatCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCa                                                    <  2:736119/140‑1 (MQ=255)                                                                                 atCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCg                                                >  1:870588/1‑140 (MQ=255)                                                                                    aCGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAg                                                       >  2:458107/1‑130 (MQ=255)                                                                                    aCGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAg                                                       <  1:458107/130‑1 (MQ=255)                                                                                                      ccGCCTCATGCGGCGAATGTGGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGCAcg                           <  2:893853/140‑1 (MQ=255)                                                                                                                       tgtgGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGCACGCGAATTGGCGTTAAAGa          >  2:504206/1‑140 (MQ=255)                                                                                                                       tgtgGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGCACGCGAATTGGCGTTAAAGa          <  1:82906/140‑1 (MQ=255)                                                                                                                       tgtgGGAATTGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGCACGCGAATTGGCGTTAAAGa          <  1:415743/140‑1 (MQ=255)                                                                                                                               ttGCCCAGGTGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGCACGCGAATTGGCGTTAAAGACGGCTCaa  >  2:979717/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   AGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTCCTGTGGATAACCATGTGTATTAGAGTTAGAAAACACGAGGCAAGCGAGAGAATACGCGGCTTGCACGCGAATTGGCGTTAAAGACGGCTCAA  >  NC_000913/812946‑813211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑