Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	899,749	A→G	intergenic (+104/+95)	rlmC → / ← artJ	23S rRNA m(5)U747 methyltransferase/L‑arginine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	899,749	0	A	G	100.0%	110.4 / NA	31	intergenic (+104/+95)	rlmC/artJ	23S rRNA m(5)U747 methyltransferase/L‑arginine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/17);  total (14/17)

GAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTG  >  NC_000913/899616‑899876                                                                                                                                      |                                                                                                                                gAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGc                                                                                                                           <  1:556320/140‑1 (MQ=255)  aaGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCa                                                                                                                          <  1:115904/140‑1 (MQ=255)      gCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATggg                                                                                                                      <  1:441487/140‑1 (MQ=255)        tGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCa                                                                                                                          <  2:686682/134‑1 (MQ=255)        tGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCa                                                                                                                          >  1:686682/1‑134 (MQ=255)        tGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATggg                                                                                                                      <  1:261263/138‑1 (MQ=255)        tGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATggg                                                                                                                      >  2:261263/1‑138 (MQ=255)        tGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGcc                                                                                                                    >  1:783320/1‑140 (MQ=255)          aCGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATggg                                                                                                                      >  2:37581/1‑136 (MQ=255)          aCGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATggg                                                                                                                      <  1:37581/136‑1 (MQ=255)           cGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATg                                                                                                                 <  1:983510/140‑1 (MQ=255)                ctgGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGc                                                                                                            <  2:133655/140‑1 (MQ=255)                ctgGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGc                                                                                                            <  1:603486/140‑1 (MQ=255)                     gAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGAt                                                                                                       <  1:401198/140‑1 (MQ=255)                                      gCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                      <  1:729321/140‑1 (MQ=255)                                                      aTTGCCGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGtc                                                                      <  1:372936/140‑1 (MQ=255)                                                           tGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAg                                                                 >  2:409307/1‑140 (MQ=255)                                                              cAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGtct                                                              <  2:820724/140‑1 (MQ=255)                                                                                  ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCttt                                          >  2:164897/1‑140 (MQ=255)                                                                                    ggATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCt                                        >  1:648603/1‑140 (MQ=255)                                                                                    ggATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCt                                        >  2:320512/1‑140 (MQ=255)                                                                                      aTGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGa                                      >  1:253093/1‑140 (MQ=255)                                                                                          ggcgTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCt                                  <  1:306330/140‑1 (MQ=255)                                                                                              tAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACt                              <  2:783320/140‑1 (MQ=255)                                                                                                     cTTATCCGGCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGaa                       <  2:261656/140‑1 (MQ=255)                                                                                                             gCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACtgtg                           >  1:107304/1‑128 (MQ=255)                                                                                                             gCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACtgtg                           <  2:107304/128‑1 (MQ=255)                                                                                                             gCCTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGt               >  2:700893/1‑140 (MQ=255)                                                                                                              ccTACAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTc              >  1:50570/1‑140 (MQ=255)                                                                                                                  cAAAAGCGTGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTg          >  1:667452/1‑140 (MQ=255)                                                                                                                          tGCAAATTCAATGCATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTg  >  1:774241/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                GAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTG  >  NC_000913/899616‑899876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑