Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	955,315	C→T	D440N (GAT→AAT)	ycaO ←	ribosomal protein S12 methylthiotransferase accessory factor YcaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	955,315	0	C	T	100.0%	183.1 / NA	51	D440N (GAT→AAT)	ycaO	ribosomal protein S12 methylthiotransferase accessory factor YcaO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/26);  total (25/26)

TCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGCTGCCCATACTGTTATTCGCGAGCCACAGATCTTCAGCCGG  >  NC_000913/955180‑955450                                                                                                                                        |                                                                                                                                        tCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTaaaa                                                                                                                                     <  2:668271/140‑1 (MQ=255) tCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCA‑GGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAAc                                                                                                                                    >  1:979651/1‑140 (MQ=255)  ccagaccagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAAc                                                                                                                                    <  1:322316/140‑1 (MQ=255)   cagaccagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAAcc                                                                                                                                   >  1:689446/1‑140 (MQ=255)     gaccagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACctt                                                                                                                                 <  1:736276/140‑1 (MQ=255)           agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCAt                                                                                                                           >  2:993337/1‑140 (MQ=255)           agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCAt                                                                                                                           >  2:728935/1‑140 (MQ=255)           agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCAt                                                                                                                           >  2:645761/1‑140 (MQ=255)           agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCAt                                                                                                                           >  2:215914/1‑140 (MQ=255)           agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCAt                                                                                                                           >  1:959253/1‑140 (MQ=255)           agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCAt                                                                                                                           >  1:869177/1‑140 (MQ=255)               tGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAg                                                                                                                       <  2:696219/140‑1 (MQ=255)                gTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTaa                                                                                                                                       >  2:1010905/1‑123 (MQ=255)                 ttCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGtt                                                                                                                     <  2:213487/140‑1 (MQ=255)                  tCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGATAAAGTCATTAAAACCTTCTTCATCCAGTTg                                                                                                                    >  2:572062/1‑140 (MQ=255)                        tCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGAt                                                                                                              <  1:645761/140‑1 (MQ=255)                        tCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGAt                                                                                                              <  2:861178/140‑1 (MQ=255)                         cGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATg                                                                                                             <  2:654957/140‑1 (MQ=255)                           ccaccaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGa                                                                                                               >  2:356618/1‑136 (MQ=255)                           ccaccaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGa                                                                                                               <  1:356618/136‑1 (MQ=255)                           ccaccaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAg                                                                                                           >  2:754182/1‑140 (MQ=255)                                 gccagcgccagcATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGa                                                                                                               <  1:905397/130‑1 (MQ=255)                                 gccagcgccagcATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGa                                                                                                               >  2:905397/1‑130 (MQ=255)                                                gCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTc                                                                                      >  2:175566/1‑140 (MQ=255)                                                    ttAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCAc                                                                                  <  2:869177/140‑1 (MQ=255)                                                       aTTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCg                                                                               <  1:215914/140‑1 (MQ=255)                                                        ttCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGc                                                                              <  2:549363/140‑1 (MQ=255)                                                            ccGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGcc                                                                          >  1:943858/1‑140 (MQ=255)                                                             cGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCt                                                                         <  2:869178/140‑1 (MQ=255)                                                                tACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGATAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGt                                                                      <  1:572062/140‑1 (MQ=255)                                                                     aGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGa                                                                 >  2:297266/1‑140 (MQ=255)                                                                         tgtACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGa                                                             >  1:714572/1‑140 (MQ=255)                                                                                aCCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGttt                                                      <  1:754182/140‑1 (MQ=255)                                                                                 ccGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTc                                                     >  2:245457/1‑140 (MQ=255)                                                                                 ccGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTc                                                     >  1:548629/1‑140 (MQ=255)                                                                                 ccGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTc                                                     >  2:810383/1‑140 (MQ=255)                                                                                   gTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCAc                                                   >  1:602410/1‑140 (MQ=255)                                                                                     tATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGt                                                 >  1:606992/1‑140 (MQ=255)                                                                                      aTCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTa                                                <  2:352382/140‑1 (MQ=255)                                                                                      aTCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTa                                                >  1:52551/1‑140 (MQ=255)                                                                                           aCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATgg                                           >  1:43491/1‑140 (MQ=255)                                                                                            cccGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGc                                          <  2:943858/140‑1 (MQ=255)                                                                                            cccGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGc                                          <  1:810383/140‑1 (MQ=255)                                                                                            cccGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGc                                          <  1:245457/140‑1 (MQ=255)                                                                                            cccGGTCGCCAGACCCAACAGCTCACGCACGCGGGGAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGc                                          >  2:508192/1‑140 (MQ=255)                                                                                               ggTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGCTGc                                       <  1:297266/140‑1 (MQ=255)                                                                                               ggTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGCTGc                                       <  2:43491/140‑1 (MQ=255)                                                                                                 tCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGCTGccc                                     >  2:631205/1‑140 (MQ=255)                                                                                                           cAACAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCCTCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTACACGTAAATGGCTGCCCATACTGTTAt                           <  2:979651/140‑1 (MQ=255)                                                                                                              cAGCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGCTGCCCATACTGTTATTcg                        <  2:548629/140‑1 (MQ=255)                                                                                                                gCTCACGCACGCGGGTAAAGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGCTGCCCATACTGTTATTcgcg                      <  2:606992/140‑1 (MQ=255)                                                                                                                                 aaGTCATTAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGCTGCCCATACTGTTATTCGCGAGCCACAGATCTTCAGc     <  1:728935/140‑1 (MQ=255)                                                                                                                                    tcattaAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGCTGCCCATACTGTTATTCGCGAGCCACAGATCTTCAGCCgg  >  1:332434/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTAAATGGCTGCCCATACTGTTATTCGCGAGCCACAGATCTTCAGCCGG  >  NC_000913/955180‑955450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑