Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	161,822	A→G	P70P (CCT→CCC)	thpR ←	RNA 2',3'‑cyclic phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	161,822	0	A	G	100.0%	153.2 / NA	44	P70P (CCT→CCC)	thpR	RNA 2',3'‑cyclic phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/16);  total (28/16)

AACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGAAGTGTGTGGC  >  NC_000913/161689‑161956                                                                                                                                      |                                                                                                                                       aacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGa                                                                                                                                  <  2:953331/140‑1 (MQ=255)                    tgAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTaaaa                                                                                                               <  1:1023761/140‑1 (MQ=255)                           aGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAg                                                                                                        >  2:165290/1‑140 (MQ=255)                              atatTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcgc                                                                                                     >  2:423525/1‑140 (MQ=255)                                       aGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCttt                                                                                            >  1:119747/1‑140 (MQ=255)                                            gATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcg                                                                                                      >  1:356676/1‑125 (MQ=255)                                            gATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcg                                                                                                      <  2:356676/125‑1 (MQ=255)                                                  gCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCa                                                                                 >  1:686399/1‑140 (MQ=255)                                                   ccGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCAc                                                                                <  1:621951/140‑1 (MQ=255)                                                      cgTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTc                                                                             >  1:278143/1‑140 (MQ=255)                                                        tGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGc                                                                           <  1:423525/140‑1 (MQ=255)                                                        tGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGc                                                                           <  2:119747/140‑1 (MQ=255)                                                                 aCGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATg                                                                  >  2:515766/1‑140 (MQ=255)                                                                           aaCCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTc                                                          <  1:769834/138‑1 (MQ=255)                                                                           aaCCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTc                                                          >  2:769834/1‑138 (MQ=255)                                                                           aaCCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAg                                                        >  1:617332/1‑140 (MQ=255)                                                                           aaCCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAg                                                        >  1:539116/1‑140 (MQ=255)                                                                                 accacacGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaa                                                  >  1:606530/1‑140 (MQ=255)                                                                                 accacacGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaa                                                  >  1:143438/1‑140 (MQ=255)                                                                                 accacacGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaa                                                  >  1:961214/1‑140 (MQ=255)                                                                                 accacacGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaa                                                  >  2:589412/1‑140 (MQ=255)                                                                                 accacacGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaa                                                  >  2:451520/1‑140 (MQ=255)                                                                                 accacacGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaa                                                  >  1:130189/1‑140 (MQ=255)                                                                                 accacacGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaa                                                  >  2:261504/1‑140 (MQ=255)                                                                                 accacacGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTAAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaa                                                  >  2:905027/1‑140 (MQ=255)                                                                                     cacGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTa                                              >  2:412005/1‑140 (MQ=255)                                                                                      acGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTAt                                             >  2:463201/1‑140 (MQ=255)                                                                                                      tGTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACg                             >  2:785259/1‑140 (MQ=255)                                                                                                       gTCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGt                            <  2:278143/140‑1 (MQ=255)                                                                                                        tCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTc                           >  2:637092/1‑140 (MQ=255)                                                                                                        tCCGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTc                           >  2:697657/1‑140 (MQ=255)                                                                                                         ccGGCGTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTcc                          <  1:697657/140‑1 (MQ=255)                                                                                                              gTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCGGAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCt                     <  1:936544/140‑1 (MQ=255)                                                                                                              gTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGGGACCGGACGTCCCGCCt                     <  2:615618/140‑1 (MQ=255)                                                                                                              gTCATCAAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCt                     <  2:617332/140‑1 (MQ=255)                                                                                                                   caAGCGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGt                <  1:589412/140‑1 (MQ=255)                                                                                                                       cGTGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGa            >  1:100812/1‑140 (MQ=255)                                                                                                                       cGTAAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGa            <  1:905027/140‑1 (MQ=255)                                                                                                                         tGAGTGTGAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGAAg          >  2:149055/1‑140 (MQ=255)                                                                                                                             tgtgAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCt                     <  2:1042048/125‑1 (MQ=255)                                                                                                                             tgtgAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCt                     >  1:1042048/1‑125 (MQ=255)                                                                                                                               tgAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGAAGtgtgtg    <  2:686399/140‑1 (MQ=255)                                                                                                                               tgAAACCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGAAGtgtgtg    >  2:915867/1‑140 (MQ=255)                                                                                                                                 aaaCCGGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGAAGTGTGTGgc  >  1:594095/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       AACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGAAGTGTGTGGC  >  NC_000913/161689‑161956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑