Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,152,953	A→G	T339A (ACC→GCC)	fabF →	beta‑ketoacyl‑[acyl carrier protein] synthase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,152,953	0	A	G	100.0%	89.6 / NA	26	T339A (ACC→GCC)	fabF	beta‑ketoacyl‑[acyl carrier protein] synthase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/20);  total (6/20)

TGAAGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCG  >  NC_000913/1152817‑1153067                                                                                                                                         |                                                                                                                   tGAAGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCg                                                                                                                 <  1:63379/140‑1 (MQ=255)  gAAGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCgg                                                                                                                >  1:78676/1‑140 (MQ=255)   aaGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGt                                                                                                               <  1:114476/140‑1 (MQ=255)   aaGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGt                                                                                                               <  2:78676/140‑1 (MQ=255)    aGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGGTCCACGAAATCTATGGCCGGTc                                                                                                              <  2:754772/140‑1 (MQ=255)        aGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCt                                                                                                          >  2:712307/1‑140 (MQ=255)         gTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTg                                                                                                         >  1:24604/1‑140 (MQ=255)          tCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGt                                                                                                        <  2:828285/140‑1 (MQ=255)          tCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGt                                                                                                        <  1:712307/140‑1 (MQ=255)                    tACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTgcggc                                                                                              <  2:1022270/140‑1 (MQ=255)                            cgcgCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCgggggggg                                                                                      >  2:84597/1‑135 (MQ=255)                            cgcgCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAg                                                                                      <  2:291818/140‑1 (MQ=255)                            cgcgCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAg                                                                                      <  2:329629/140‑1 (MQ=255)                             gcgcACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCATGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAGt                                                                                     <  2:716781/140‑1 (MQ=255)                                       cTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAatctatc                                                                           <  2:239297/140‑1 (MQ=255)                                          cTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTAc                                                                        <  1:519944/140‑1 (MQ=255)                                           tACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAA‑CTATCTACTc                                                                      <  1:272975/140‑1 (MQ=255)                                                                tGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATc                                                  <  1:84597/140‑1 (MQ=255)                                                                 gAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCa                                                 <  1:364645/140‑1 (MQ=255)                                                                       cAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCAGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGt                                           >  1:895675/1‑140 (MQ=255)                                                                             gTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGcc                                     <  1:236312/140‑1 (MQ=255)                                                                                 aaaCCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAAcc                                 <  1:310823/140‑1 (MQ=255)                                                                                  aaCCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCa                                <  2:948860/140‑1 (MQ=255)                                                                                  aaCCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCAGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCa                                <  2:895675/140‑1 (MQ=255)                                                                                                              gtgtTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTg    <  1:519462/140‑1 (MQ=255)                                                                                                                gtTGGTAAGCTCCACGAAATCTATGGCCGGTCACCTGTTAGGTGCGGCGGGGGCAGGAAAAATTATATATTCCCTCCTCGCGCTGCGCGGGCAGGGTGTTTCCCCCACCACCCACCCCGGTAAACCCGGTGAAGGGTGCg  >  2:31461/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                   TGAAGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCG  >  NC_000913/1152817‑1153067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑