Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,225,935	A→G	V49A (GTA→GCA)	minC ←	Z‑ring positioning protein MinC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,225,935	0	A	G	100.0%	235.0 / NA	65	V49A (GTA→GCA)	minC	Z‑ring positioning protein MinC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/31);  total (34/31)

CCCATCTTTTCAATTTCGGCTTTAAGTTGCGCATCTTTGCAGCCACTTACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGT  >  NC_000913/1225800‑1226071                                                                                                                                        |                                                                                                                                         cccATCTTTTCAATTTCGGCTTTAAGTTGCGCATCTTTGCAGCCACTTACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAAc                                                                                                                                      >  2:958887/1‑140 (MQ=255)      cTTTTCAATTTCGGCTTTAAGTTGCGCATCTTTGCAGCCACTTACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACggggg                                                                                                                                 <  1:430940/140‑1 (MQ=255)          tCAATTTCGGCTTTAAGTTGCGCATCTTTGCAGCCACTTACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATg                                                                                                                             >  2:348061/1‑140 (MQ=255)                   gCTTTAAGTTGCGCATCTTTGCAGCCACTTACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTaaaaa                                                                                                                    >  1:991334/1‑140 (MQ=255)                       tAAGTTGCGCATCTTTGCAGCCACTTACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCg                                                                                                                <  1:768262/140‑1 (MQ=255)                            tGCGCATCTTTGCAGCCACTTACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGc                                                                                                           <  2:476223/140‑1 (MQ=255)                            tGCGCATCTTTGCAGCCACTTACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGc                                                                                                           <  2:641365/140‑1 (MQ=255)                               gcATCTTTGCAGCCACTTACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTg                                                                                                        >  1:670908/1‑140 (MQ=255)                                             aCTTACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCtt                                                                                          >  2:11361/1‑140 (MQ=255)                                               ttACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTcc                                                                                        <  2:670908/140‑1 (MQ=255)                                               ttACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTcc                                                                                        >  2:203250/1‑140 (MQ=255)                                                tACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCa                                                                                       >  2:613658/1‑140 (MQ=255)                                                     cAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGTATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGcc                                                                                  <  2:805627/140‑1 (MQ=255)                                                     cAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGcc                                                                                  <  2:93633/140‑1 (MQ=255)                                                       aTAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTg                                                                                >  2:989113/1‑140 (MQ=255)                                                       aTAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTg                                                                                >  1:794966/1‑140 (MQ=255)                                                        tAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGAtt                                                                                                 <  1:682483/122‑1 (MQ=255)                                                        tAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGAtt                                                                                                 >  2:682483/1‑122 (MQ=255)                                                        tAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGa                                                                               >  1:445098/1‑140 (MQ=255)                                                            ccGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGa                                                                           <  1:613658/140‑1 (MQ=255)                                                            ccGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGa                                                                           <  2:883188/140‑1 (MQ=255)                                                             cGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCt                                                                                 <  2:467067/133‑1 (MQ=255)                                                             cGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCt                                                                                 >  1:467067/1‑133 (MQ=255)                                                             cGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGa                                                                           <  2:353238/139‑1 (MQ=255)                                                             cGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGa                                                                           >  1:353238/1‑139 (MQ=255)                                                              gCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATa                                                                         <  1:301821/140‑1 (MQ=255)                                                                      gTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAgg                                                                 >  1:972843/1‑140 (MQ=255)                                                                        tGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGtt                                                               <  2:794966/140‑1 (MQ=255)                                                                        tGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGtt                                                               <  1:309082/140‑1 (MQ=255)                                                                         gCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTc                                                              >  1:638537/1‑140 (MQ=255)                                                                             aaaCCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGcc                                                          <  2:602719/140‑1 (MQ=255)                                                                             aaaCCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGcc                                                          <  2:393627/140‑1 (MQ=255)                                                                             aaaCCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGcc                                                          <  2:857751/140‑1 (MQ=255)                                                                                 cGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCAt                                                      <  2:445098/140‑1 (MQ=255)                                                                                    cTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCa                                                   >  1:103818/1‑140 (MQ=255)                                                                                      tATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGa                                                 <  2:638537/140‑1 (MQ=255)                                                                                       aTGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGAt                                                <  1:203250/140‑1 (MQ=255)                                                                                        tGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGtt                                                               <  2:712248/124‑1 (MQ=255)                                                                                        tGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGtt                                                               >  1:712248/1‑124 (MQ=255)                                                                                           aTCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAAc                                            <  2:594039/140‑1 (MQ=255)                                                                                             cGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACca                                          >  2:33604/1‑140 (MQ=255)                                                                                             cGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACca                                          >  2:761567/1‑140 (MQ=255)                                                                                              gCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACcac                                         >  2:775057/1‑140 (MQ=255)                                                                                              gCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACcac                                         >  1:92796/1‑140 (MQ=255)                                                                                                  aCCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGAt                                                <  1:928966/129‑1 (MQ=255)                                                                                                  aCCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGAt                                                >  2:928966/1‑129 (MQ=255)                                                                                                  aCCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGAt                                     >  2:461322/1‑140 (MQ=255)                                                                                                  aCCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGAt                                     <  1:775057/140‑1 (MQ=255)                                                                                                    cAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATaa                                   <  1:989113/140‑1 (MQ=255)                                                                                                       tttACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGt                                >  1:446993/1‑140 (MQ=255)                                                                                                        ttACCGGGTCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTg                               <  1:761567/140‑1 (MQ=255)                                                                                                                tCTTCCAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACt                       <  1:958887/140‑1 (MQ=255)                                                                                                                     cAGTGCACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCtt                  >  1:776311/1‑140 (MQ=255)                                                                                                                          cACTGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGc             >  1:161509/1‑140 (MQ=255)                                                                                                                             tGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTa                <  2:340744/134‑1 (MQ=255)                                                                                                                             tGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTa                >  1:340744/1‑134 (MQ=255)                                                                                                                             tGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCg          >  2:119501/1‑140 (MQ=255)                                                                                                                             tGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCg          >  1:901877/1‑140 (MQ=255)                                                                                                                             tGACGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCg          >  1:217960/1‑140 (MQ=255)                                                                                                                               aCGTTGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGAt        >  2:818204/1‑140 (MQ=255)                                                                                                                                  ttGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGAt        >  1:915110/1‑137 (MQ=255)                                                                                                                                  ttGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGAt        <  2:915110/137‑1 (MQ=255)                                                                                                                                  ttGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTgg     >  2:528466/1‑140 (MQ=255)                                                                                                                                  ttGAGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTgg     >  1:75480/1‑140 (MQ=255)                                                                                                                                     aGTGCAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCgt  <  2:92796/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CCCATCTTTTCAATTTCGGCTTTAAGTTGCGCATCTTTGCAGCCACTTACGCCAATAACCCGCAAACCGGTTGCCGAAACCGCCTTATGCATCGCTGACCAGTTTACCGGGTCTTCCAGTGCACTGACGTTGAGTACAACGGGGGCATGTTTTAAAAATGCGGGGGCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGT  >  NC_000913/1225800‑1226071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑