Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,417,580	A→G	C62R (TGC→CGC)	racC ←	Rac prophage; protein RacC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,417,580	0	A	G	100.0%	199.9 / NA	55	C62R (TGC→CGC)	racC	Rac prophage; protein RacC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/27);  total (28/27)

TACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTTTCCTTCCAGATTCACCTCGTG  >  NC_000913/1417446‑1417715                                                                                                                                        |                                                                                                                                        tACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCAttt                                                                                                                                    >  1:727850/1‑140 (MQ=255)      acTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTg                                                                                                                               >  2:819606/1‑140 (MQ=255)       cTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGa                                                                                                                              <  2:580565/140‑1 (MQ=255)        tGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGac                                                                                                                             >  1:144992/1‑140 (MQ=255)         gTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGaca                                                                                                                            >  1:98847/1‑140 (MQ=255)         gTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGaca                                                                                                                            >  2:407402/1‑140 (MQ=255)          ttCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGaca                                                                                                                            <  1:931589/140‑1 (MQ=255)          ttCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGacac                                                                                                                           >  1:306837/1‑140 (MQ=255)          ttCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGacac                                                                                                                           >  2:179980/1‑140 (MQ=255)          ttCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGacac                                                                                                                           >  1:444966/1‑140 (MQ=255)          ttCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGacac                                                                                                                           >  1:632792/1‑140 (MQ=255)                 ccTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTtcatc                                                                                                                    >  1:1041267/1‑140 (MQ=255)                  cTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTtcatca                                                                                                                   >  2:99712/1‑140 (MQ=255)                  cTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTtcatca                                                                                                                   <  2:144992/140‑1 (MQ=255)                    gcgcCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAg                                                                                                                 >  1:475688/1‑140 (MQ=255)                    gcgcCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAg                                                                                                                 >  2:663714/1‑140 (MQ=255)                          tACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGtttt                                                                                                           >  1:646729/1‑140 (MQ=255)                                ggCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGtt                                                                                                     <  2:444966/140‑1 (MQ=255)                                    aTTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACg                                                                                                       <  2:607141/134‑1 (MQ=255)                                    aTTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACg                                                                                                       >  1:607141/1‑134 (MQ=255)                                     tttctttcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTg                                                                                                >  2:981984/1‑140 (MQ=255)                                       tctttcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGAt                                                                                              <  1:538297/140‑1 (MQ=255)                                           tcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGAc                                                                                          >  1:921775/1‑140 (MQ=255)                                             aGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCa                                                                                        <  2:306837/140‑1 (MQ=255)                                             aGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCa                                                                                        >  1:415420/1‑140 (MQ=255)                                               ttttCACCTTTTAATTTCCTTGCAATTAGAGTTGCCAGACATTCGGC‑TTTTTTTTCTGCGGGAAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACATCTTCATCAAGTGTTTTTACGTTGTCTGATGGACCATc                                                                                      >  2:868075/2‑140 (MQ=255)                                               aTTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATc                                                                                      >  1:600957/1‑140 (MQ=255)                                               aTTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATc                                                                                      >  1:508085/1‑140 (MQ=255)                                                tttCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCg                                                                                     <  1:99712/140‑1 (MQ=255)                                                 ttCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGa                                                                                    >  1:85574/1‑140 (MQ=255)                                                  tCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATgg                                                                                            <  2:93147/131‑1 (MQ=255)                                                  tCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATgg                                                                                            >  1:93147/1‑131 (MQ=255)                                                  tCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGat                                                                                   <  1:296986/140‑1 (MQ=255)                                                  tCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGat                                                                                   <  2:896187/140‑1 (MQ=255)                                                            aTTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACcaca                                                                         <  1:663714/140‑1 (MQ=255)                                                            aTTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACcaca                                                                         <  1:452573/140‑1 (MQ=255)                                                            aTTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACcaca                                                                         <  1:981984/140‑1 (MQ=255)                                                                 aTTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGaa                                                                    <  1:745470/140‑1 (MQ=255)                                                                  ttGCAATTAGAGTTGCCAGAATTTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAAt                                                                   >  2:743595/1‑140 (MQ=255)                                                                   tGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATg                                                                  >  1:708376/1‑140 (MQ=255)                                                                       aTTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGt                                                              <  2:600957/140‑1 (MQ=255)                                                                        ttAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGtt                                                             <  1:819606/140‑1 (MQ=255)                                                                                 ccAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTAttt                                                    <  1:254055/140‑1 (MQ=255)                                                                                 ccAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTAttt                                                    <  2:646729/140‑1 (MQ=255)                                                                                                  tttttCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGt                                    <  2:239218/140‑1 (MQ=255)                                                                                                  tttttCTGCGGGCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGt                                    <  1:407402/140‑1 (MQ=255)                                                                                                           gggCAGATTCTTTCCGATATGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTAAGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATgc                           >  2:406167/1‑140 (MQ=255)                                                                                                                           atatGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTTTCCTTCCAGAtt           >  2:270786/1‑140 (MQ=255)                                                                                                                           atatGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTTTCCTTCCAGAtt           >  2:563526/1‑140 (MQ=255)                                                                                                                            tatGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTTTCCTTCCAGATTc          <  1:139589/140‑1 (MQ=255)                                                                                                                              tGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTTTCCTTCCAGATTCAc        <  1:563526/140‑1 (MQ=255)                                                                                                                              tGCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTTTCCTTCCAGATTCAc        <  1:270786/140‑1 (MQ=255)                                                                                                                               gCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTttccttc                 >  2:634770/1‑130 (MQ=255)                                                                                                                               gCACCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTttccttc                 <  1:634770/130‑1 (MQ=255)                                                                                                                                 aCCAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTTTCCTTCCAGATTCACCTc     <  2:415420/140‑1 (MQ=255)                                                                                                                                   cAGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTTTCCTTCCAGATTCACCTCGt   >  1:760698/1‑140 (MQ=255)                                                                                                                                    aGGCGCATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTTTCCTTCCAGATTCACCTCGTg  <  2:508085/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTCTGTTTTAATTACGTAGCCAATGCGCTTTCCTTCCAGATTCACCTCGTG  >  NC_000913/1417446‑1417715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑