Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,486,271	C→T	R1071C (CGC→TGC)	hrpA →	ATP‑dependent RNA helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,486,271	0	C	T	100.0%	126.2 / NA	36	R1071C (CGC→TGC)	hrpA	ATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/23);  total (13/23)

CTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGG  >  NC_000913/1486133‑1486407                                                                                                                                           |                                                                                                                                         cTGCCGGAAAGCTACGAACAGAAGCGTGGC‑ACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGc                                                                                                                                        >  2:836041/1‑140 (MQ=255)    ccGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCg                                                                                                                                      <  1:995063/140‑1 (MQ=255)      ggAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTc                                                                                                                                    <  1:470310/140‑1 (MQ=255)              aCGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTActgctg                                                                                                                            <  2:616727/140‑1 (MQ=255)                           tGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCAt                                                                                                               >  1:276642/1‑140 (MQ=255)                             gCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCg                                                                                                             <  1:424006/140‑1 (MQ=255)                               aaCTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGcc                                                                                                           <  1:81868/140‑1 (MQ=255)                                  tACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAAt                                                                                                        <  1:940441/140‑1 (MQ=255)                                          gAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATAtt                                                                                                <  1:232023/140‑1 (MQ=255)                                          gAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATAtt                                                                                                <  1:836041/140‑1 (MQ=255)                                                   gCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGaaa                                                                                       >  2:309224/1‑140 (MQ=255)                                                   gCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGaaa                                                                                       >  1:1004267/1‑140 (MQ=255)                                                       gcgcTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAAACCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGtt                                                                                   >  2:148067/1‑140 (MQ=255)                                                             gtggATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAGCGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGaa                                                                             >  1:467136/1‑140 (MQ=255)                                                                   gAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGc                                                                       <  2:270326/140‑1 (MQ=255)                                                                          aCAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTg                                                                >  2:496072/1‑140 (MQ=255)                                                                          aCAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTg                                                                <  2:276642/140‑1 (MQ=255)                                                                              tgtgGCGATCTAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGAc                                                            <  1:979105/140‑1 (MQ=255)                                                                              tgtgGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGAc                                                            >  2:723535/1‑140 (MQ=255)                                                                                tgGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTg                                                          >  1:339028/1‑140 (MQ=255)                                                                                     aTCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACtt                                                     >  2:125889/1‑140 (MQ=255)                                                                                                 gATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATgg                                         <  2:339028/140‑1 (MQ=255)                                                                                                   tAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCa                                       >  1:784356/1‑140 (MQ=255)                                                                                                   tAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCa                                       <  1:723535/140‑1 (MQ=255)                                                                                                    aaCCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCaa                                      <  2:1004267/140‑1 (MQ=255)                                                                                                              aGCAAAAGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAg                            <  2:118556/140‑1 (MQ=255)                                                                                                                   aaGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGAt                       >  1:125159/1‑140 (MQ=255)                                                                                                                    aGCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATc                      <  1:309224/140‑1 (MQ=255)                                                                                                                     gCAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATcg                     <  1:524377/140‑1 (MQ=255)                                                                                                                      cAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATcga                    <  2:467136/140‑1 (MQ=255)                                                                                                                      cAGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATcga                    <  1:496072/140‑1 (MQ=255)                                                                                                                       aGGCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATcgac                   <  2:784356/140‑1 (MQ=255)                                                                                                                        ggCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATcgacg                  <  2:186117/140‑1 (MQ=255)                                                                                                                         gCAATGTGGAACGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATcgacga                 >  2:154360/1‑140 (MQ=255)                                                                                                                                    cGGTCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACAACTGTATCTCCt      <  1:148067/140‑1 (MQ=255)                                                                                                                                       tCTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCg   >  2:168612/1‑140 (MQ=255)                                                                                                                                        cTTTGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCgg  <  2:125159/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         CTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGG  >  NC_000913/1486133‑1486407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑