Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,613,791	T→C	N305D (AAC→GAC)	sad ←	NAD(+)‑dependent succinate semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,613,791	0	T	C	100.0%	150.6 / NA	42	N305D (AAC→GAC)	sad	NAD(+)‑dependent succinate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/18);  total (24/18)

AGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAAC  >  NC_000913/1613665‑1613925                                                                                                                               |                                                                                                                                       agtagtTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACgg                                                                                                                           >  2:849338/1‑140 (MQ=255)         ccTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCCCCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGcc                                                                                                                   <  1:68431/140‑1 (MQ=255)             ccccAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCAtt                                                                                                               >  1:911309/1‑140 (MQ=255)                  gCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCaa                                                                                                          >  2:141060/1‑140 (MQ=255)                             cccGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAg                                                                                               <  1:849338/140‑1 (MQ=255)                                gCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTg                                                                                            >  2:527557/1‑140 (MQ=255)                                           aaCGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGt                                                                                 <  1:141060/140‑1 (MQ=255)                                            aCGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGtt                                                                                <  1:527557/140‑1 (MQ=255)                                               cgcACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCgg                                                                             >  1:16230/1‑140 (MQ=255)                                                gcACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGt                                                                            >  1:982217/1‑140 (MQ=255)                                                gcACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGt                                                                            >  1:656504/1‑140 (MQ=255)                                                 cACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTa                                                                           >  1:851441/1‑140 (MQ=255)                                                    ccTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAAt                                                                        >  2:535044/1‑140 (MQ=255)                                                      tGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGc                                                                      >  2:606680/1‑140 (MQ=255)                                                                   tctcCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTccc                                                         <  1:606680/140‑1 (MQ=255)                                                                   tctcCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTccc                                                         <  2:437295/140‑1 (MQ=255)                                                                        aCCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTc                                                    >  1:970414/1‑140 (MQ=255)                                                                            gatgatGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGata                                                >  2:189420/1‑140 (MQ=255)                                                                             atgatgCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGataa                                               >  1:538238/1‑140 (MQ=255)                                                                             atgatgCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGataa                                               >  1:192151/1‑140 (MQ=255)                                                                                  gCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAg                                          <  2:64922/140‑1 (MQ=255)                                                                                    aGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTcc                                                          >  2:363689/1‑122 (MQ=255)                                                                                    aGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTcc                                                          <  1:363689/122‑1 (MQ=255)                                                                                    aGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCg                                        >  2:480615/1‑140 (MQ=255)                                                                                    aGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCg                                        >  2:620074/1‑140 (MQ=255)                                                                                       tcatcaCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGttt                                     >  2:552032/1‑140 (MQ=255)                                                                                       tcatcaCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGttt                                     >  2:657863/1‑140 (MQ=255)                                                                                       tcatcaCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGttt                                     <  1:480615/140‑1 (MQ=255)                                                                                        catcaCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGtttt                                    >  1:337557/1‑140 (MQ=255)                                                                                         atcaCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTg                                   >  1:1033521/1‑140 (MQ=255)                                                                                           caCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGcc                                 <  1:657863/140‑1 (MQ=255)                                                                                            aCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCg                                <  2:656504/140‑1 (MQ=255)                                                                                            aCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCg                                <  1:189420/140‑1 (MQ=255)                                                                                                        aCGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCt                    <  2:911309/140‑1 (MQ=255)                                                                                                        aCGAGCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCt                    <  2:970414/140‑1 (MQ=255)                                                                                                            gCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTcc                <  2:337557/140‑1 (MQ=255)                                                                                                            gCCATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTcc                >  1:426250/1‑140 (MQ=255)                                                                                                             ccATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCg               <  1:535044/140‑1 (MQ=255)                                                                                                              cATTGGTCCGAGAGCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCgg              <  2:538238/140‑1 (MQ=255)                                                                                                                         gagCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATaa   <  1:929403/140‑1 (MQ=255)                                                                                                                          agCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCTGCTGCACATACCTGTCCGGTATTCTGATAAc  >  1:112379/1‑140 (MQ=255)                                                                                                                          agCGTCCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAAc  >  1:968924/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                       AGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGCCGCTGCACATACCTGTCCGGTATTCTGATAAC  >  NC_000913/1613665‑1613925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑