Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,690,397	T→C	A182A (GCT→GCC)	ydgA →	conserved protein YdgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,690,397	0	T	C	100.0%	113.3 / NA	32	A182A (GCT→GCC)	ydgA	conserved protein YdgA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

AAACGCCTTTTGAGATTAACTCGCGCATTGGTTACAGCGGTGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCTGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGCAGTTGACCTTTAATAATCTGAAAACCGACGGTTCCAGCACGCTGGCAAGT  >  NC_000913/1690264‑1690532                                                                                                                                      |                                                                                                                                        aaaCGCCTTTTGAGATTAACTCGCGCATTGGTTACAGCGGTGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACaga                                                                                                                                   >  2:199152/1‑140 (MQ=255)              gaTTAACTCGCGCATTGGTTACAGCGGTGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCa                                                                                                                      <  1:374508/140‑1 (MQ=255)                    cTCGCGCATTGGTTACAGCGGTGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTccc                                                                                                                >  1:553996/1‑140 (MQ=255)                         gcATTGGTTACAGCGGTGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTcc                                                                                                           >  2:1048367/1‑140 (MQ=255)                         gcATTGGTTACAGCGGTGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTcc                                                                                                           >  2:385520/1‑140 (MQ=255)                            ttGGTTACAGCGGTGATTCCAGTTCCGATATTTCGCTCAAGCCACTCAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGTGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCggg                                                                                                        <  2:90670/140‑1 (MQ=255)                             tGGTTACAGCGGTGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCgggg                                                                                                       >  2:1026056/1‑140 (MQ=255)                                     gCGGTGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCggg                                                                                                        <  2:383929/131‑1 (MQ=255)                                     gCGGTGATTCCAGTTCCGATACTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGTCGGCAAAGCCATCTCCCTTTCCggg                                                                                                        >  1:383929/1‑131 (MQ=255)                                         tGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGCATTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGCGAGGCGCAAAGTg                                                                                           >  2:642375/1‑140 (MQ=255)                                         tGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCa                                                                                                >  2:7990/1‑135 (MQ=255)                                         tGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCa                                                                                                <  1:7990/135‑1 (MQ=255)                                          gATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTgg                                                                                          <  1:311716/140‑1 (MQ=255)                                           aTTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGt                                                                                         >  1:248149/1‑140 (MQ=255)                                           aTTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGt                                                                                         <  2:553996/140‑1 (MQ=255)                                               cAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGa                                                                                     <  1:562591/140‑1 (MQ=255)                                                   tCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGa                                                                                 <  2:248149/140‑1 (MQ=255)                                                     cGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACg                                                                               <  1:1048367/140‑1 (MQ=255)                                                     cGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACg                                                                               <  1:385520/140‑1 (MQ=255)                                                       atatTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCa                                                                             >  1:409382/1‑140 (MQ=255)                                                                tCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGaa                                                                    <  1:915356/140‑1 (MQ=255)                                                                tCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGaa                                                                    <  1:1026056/140‑1 (MQ=255)                                                                              tACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGt                                                      >  2:265661/1‑140 (MQ=255)                                                                                cGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGc                                                    >  1:915363/1‑140 (MQ=255)                                                                                     aaaaGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGCAGTTg                                               >  1:661500/1‑140 (MQ=255)                                                                                     aaaaGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGCAGTTg                                               >  2:78583/1‑140 (MQ=255)                                                                                            tGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGTGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGCAGTTGACCTTta                                        >  1:192836/1‑140 (MQ=255)                                                                                            tGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGCAGTTGACCTTta                                        >  2:381260/1‑140 (MQ=255)                                                                                              aaaaaGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGCAGTTGACCTTtaat                                      <  1:199152/140‑1 (MQ=255)                                                                                                            aGCGGCGGCGAGTTCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGCAGTTGACCTTTAATAATCTGAAAACCGa                        <  1:265661/140‑1 (MQ=255)                                                                                                                         tCCAGTTAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGCAGTTGACCTTTAATAATCTGAAAACCGACGGTTCCAGCACg           <  2:915363/140‑1 (MQ=255)                                                                                                                              ttAAATGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGCAGTTGGCCTTTAATAATCTGAAAACCGACGGTTCCAGCACGCTGGc      <  1:642375/140‑1 (MQ=255)                                                                                                                                  aTGCCGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGCAGTTGACCTTTAATAATCTGAAAACCGACGGTTCCAGCACGCTGGCAAGt  >  2:943122/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        AAACGCCTTTTGAGATTAACTCGCGCATTGGTTACAGCGGTGATTCCAGTTCCGATATTTCGCTCAAGCCACTGAATTACGAGCAAAAGGATGAAAAAGTCGCCTTTAGCGGCGGCGAGTTCCAGTTAAATGCTGACAGAGACGGCAAAGCCATCTCCCTTTCCGGGGAGGCGCAAAGTGGTCGGATAGACGCAGTTAACGAATACAACCAGAAAGTGCAGTTGACCTTTAATAATCTGAAAACCGACGGTTCCAGCACGCTGGCAAGT  >  NC_000913/1690264‑1690532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑