Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,705,638	T→C	V130A (GTC→GCC)	ydgK →	conserved inner membrane protein YdgK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,705,638	0	T	C	100.0%	160.2 / NA	45	V130A (GTC→GCC)	ydgK	conserved inner membrane protein YdgK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/20);  total (25/20)

TGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGA  >  NC_000913/1705502‑1705774                                                                                                                                         |                                                                                                                                         tgtgTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGcccc                                                                                                                                       <  1:1010319/140‑1 (MQ=255)               cTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCt                                                                                                                                     >  2:55922/1‑128 (MQ=255)               cTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCt                                                                                                                                     <  1:55922/128‑1 (MQ=255)                   attatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCtcgtc                                                                                                                     <  2:602839/140‑1 (MQ=255)                       tCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCgg                                                                                                                 >  2:77645/1‑140 (MQ=255)                          ggCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTa                                                                                                              >  1:380434/1‑140 (MQ=255)                          ggCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTa                                                                                                              >  1:69795/1‑140 (MQ=255)                          ggCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTa                                                                                                              >  1:719965/1‑140 (MQ=255)                          ggCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTa                                                                                                              >  1:810024/1‑140 (MQ=255)                            ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCtcgtc                                                                                                                     >  2:907396/1‑131 (MQ=255)                            ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCtcgtc                                                                                                                     <  1:907396/131‑1 (MQ=255)                            ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTaaa                                                                                                            >  2:733995/1‑140 (MQ=255)                            ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTaaa                                                                                                            >  2:632724/1‑140 (MQ=255)                            ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTaaa                                                                                                            >  1:952312/1‑140 (MQ=255)                            ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTaaa                                                                                                            >  2:141241/1‑140 (MQ=255)                              gctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAg                                                                                                          >  1:395902/1‑140 (MQ=255)                               ctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGc                                                                                                         >  2:888300/1‑140 (MQ=255)                               ctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGc                                                                                                         >  1:908510/1‑140 (MQ=255)                               ctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATACCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGc                                                                                                         >  2:477643/1‑140 (MQ=255)                                 tATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGa                                                                                                       >  2:445425/1‑140 (MQ=255)                                         tATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTtgtg                                                                                               >  2:1003945/1‑140 (MQ=255)                                            tGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAt                                                                                            <  2:908510/140‑1 (MQ=255)                                              cTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATcc                                                                                          >  2:444076/1‑140 (MQ=255)                                                  cAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaa                                                                                      <  1:632724/140‑1 (MQ=255)                                                   aGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaat                                                                                     >  2:386685/1‑140 (MQ=255)                                                   aGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaat                                                                                     >  1:685312/1‑140 (MQ=255)                                                        aaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCt                                                                                <  1:141241/140‑1 (MQ=255)                                                        aaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCt                                                                                <  2:380434/140‑1 (MQ=255)                                                        aaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCt                                                                                <  2:395902/140‑1 (MQ=255)                                                             ttCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGTAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAg                                                                           >  1:506355/1‑140 (MQ=255)                                                              tCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCACCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGt                                                                          >  1:570883/1‑140 (MQ=255)                                                                  tttttCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAc                                                                      <  2:687185/140‑1 (MQ=255)                                                                           cGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAc                                                                      <  1:893192/131‑1 (MQ=255)                                                                           cGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAc                                                                      >  2:893192/1‑131 (MQ=255)                                                                            gTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGc                                                            <  1:477643/140‑1 (MQ=255)                                                                            gTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGc                                                            <  1:386685/140‑1 (MQ=255)                                                                             tGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGcc                                                           <  1:444076/140‑1 (MQ=255)                                                                                 aGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGct                                                       <  1:733995/140‑1 (MQ=255)                                                                                   aTGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGctct                                                     <  2:311773/140‑1 (MQ=255)                                                                                       cATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGAt                                                 <  2:685312/140‑1 (MQ=255)                                                                                       cATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGAt                                                 <  1:156754/140‑1 (MQ=255)                                                                                                 cGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataa                                       >  2:832791/1‑140 (MQ=255)                                                                                                                              cGCATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGaaaa          >  1:47306/1‑140 (MQ=255)                                                                                                                                cATTGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAAt        <  2:47306/140‑1 (MQ=255)                                                                                                                                  ttGATCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAAtga      <  2:469189/140‑1 (MQ=255)                                                                                                                                      tCGCCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAAtgactga  <  2:273952/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGA  >  NC_000913/1705502‑1705774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑