Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,717,614	T→C	S132G (AGC→GGC)	pdxH ←	pyridoxal 5‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,717,614	0	T	C	100.0%	84.4 / NA	25	S132G (AGC→GGC)	pdxH	pyridoxal 5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/13);  total (12/13)

GCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTT  >  NC_000913/1717480‑1717743                                                                                                                                       |                                                                                                                                  gCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGaa                                                                                                                              >  2:526838/1‑140 (MQ=255)  cTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGaaa                                                                                                                             >  2:875539/1‑140 (MQ=255)   tCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGaaaa                                                                                                                            <  1:559273/140‑1 (MQ=255)          tGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTtca                                                                                                                     >  2:164636/1‑140 (MQ=255)           ggCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTtcat                                                                                                                    <  1:33103/140‑1 (MQ=255)               cTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACt                                                                                                                >  1:288253/1‑140 (MQ=255)                   gCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCga                                                                                                            <  1:164636/140‑1 (MQ=255)                                ttCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCg                                                                                               <  2:927067/140‑1 (MQ=255)                                   tGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCg                                                                                               <  1:491579/137‑1 (MQ=255)                                   tGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCg                                                                                               >  2:491579/1‑137 (MQ=255)                                   tGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTc                                                                                            >  2:101577/1‑140 (MQ=255)                                          gCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTa                                                                                     >  1:625583/1‑140 (MQ=255)                                                   aTTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGatcacc                                                                            <  2:119682/140‑1 (MQ=255)                                                         tttCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGatcaccatcacc                                                                      <  1:101577/140‑1 (MQ=255)                                                           tCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTg                                                                    <  1:875539/140‑1 (MQ=255)                                                                       cgGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTc                                                              >  2:346325/1‑134 (MQ=255)                                                                       cgGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTc                                                              <  1:346325/134‑1 (MQ=255)                                                                       cgGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTCAAGGGt                                                        >  2:804771/1‑140 (MQ=255)                                                                         ggCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTCAAGGGTAt                                                      <  2:625583/140‑1 (MQ=255)                                                                             gAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTCAAGGGTATGCCa                                                  <  2:524953/140‑1 (MQ=255)                                                                                 tGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTCAAGGGTATGCCACggg                                              <  1:464049/140‑1 (MQ=255)                                                                                              cTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTaa                                 <  2:290086/140‑1 (MQ=255)                                                                                                         aTGCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGatta                      >  2:798949/1‑140 (MQ=255)                                                                                                           gCACCAATCTGGCTATCACGCGGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGattatt                    >  2:127608/1‑140 (MQ=255)                                                                                                                             cgcgGGCGGCCATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCttt  >  2:179283/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                  GCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAGGATACCGCGGGCAGAAATGCGACTGGACTGCTTCGAAACCCATGCACCAATCTGGCTATCACGCGGGCGGCTATGAAAATATTTCATCACTTCGAGAGTCGAAAGTCGTTCTGCTTTACCGATCACCATCACCTGGCGCTCAAGGGTATGCCACGGGAACAGCAGGCTAACGCGCGGATTATTTTCGATTTGATGTGCTTT  >  NC_000913/1717480‑1717743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑