Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,720,785	G→A	R14C (CGC→TGC)	slyA ←	DNA‑binding transcriptional dual regulator SlyA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,720,785	0	G	A	100.0%	233.8 / NA	66	R14C (CGC→TGC)	slyA	DNA‑binding transcriptional dual regulator SlyA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/38);  total (28/38)

CTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGC  >  NC_000913/1720648‑1720912                                                                                                                                          |                                                                                                                                ctCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGcaca                                                                                                                               >  1:722879/1‑140 (MQ=255) ctCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGcaca                                                                                                                               >  1:582754/1‑140 (MQ=255)      tGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAAc                                                                                                                          >  2:470281/1‑140 (MQ=255)      tGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAAc                                                                                                                          <  1:822109/140‑1 (MQ=255)      tGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAAc                                                                                                                          >  2:822109/1‑140 (MQ=255)      tGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAAc                                                                                                                          <  1:470281/140‑1 (MQ=255)      tGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGATCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAAc                                                                                                                          <  2:837664/140‑1 (MQ=255)           aTCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGc                                                                                                                     <  2:505674/140‑1 (MQ=255)             cGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCa                                                                                                                   <  2:722879/140‑1 (MQ=255)                  ttGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCa                                                                                                              <  1:102590/140‑1 (MQ=255)                  ttGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCa                                                                                                              <  2:579221/140‑1 (MQ=255)                  ttGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCa                                                                                                              <  1:520331/140‑1 (MQ=255)                         ttGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTa                                                                                                       <  2:832876/140‑1 (MQ=255)                                   gACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTc                                                                                             >  1:993144/1‑140 (MQ=255)                                         tCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAAttt                                                                                       >  2:193728/1‑140 (MQ=255)                                           tGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCa                                                                                     <  1:165056/140‑1 (MQ=255)                                            ggAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCAt                                                                                    <  2:773253/140‑1 (MQ=255)                                            ggAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCAt                                                                                    <  1:832868/140‑1 (MQ=255)                                             gAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCAtc                                                                                   <  1:76222/140‑1 (MQ=255)                                                 tAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCt                                                                               <  2:993144/140‑1 (MQ=255)                                                  aaCTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCtt                                                                              >  1:967192/1‑140 (MQ=255)                                                   aCTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTta                                                                             >  2:7205/1‑140 (MQ=255)                                                   aCTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTta                                                                             >  2:273071/1‑140 (MQ=255)                                                       aTGGATATTGTGTAACGTAACCCCATGGGTTTGTGTTAACTCCAGCGGCTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTCCCAGATCAGAACCTAGTTGCGATTTCAATTTCACCCTCTTATAAt                                                                         >  2:982543/1‑140 (MQ=255)                                                          gATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAg                                                                      <  2:967192/140‑1 (MQ=255)                                                               tgtgTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGc                                                                 >  1:335614/1‑140 (MQ=255)                                                                gtgtAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCt                                                                <  2:1000582/140‑1 (MQ=255)                                                                 tgtAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCt                                                                    >  1:955954/1‑135 (MQ=255)                                                                 tgtAACGTAACCCAATAGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCt                                                                    <  2:955954/135‑1 (MQ=255)                                                                   taacgtaacCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAg                                                             >  1:298720/1‑140 (MQ=255)                                                                   taacgtaacCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAg                                                             <  1:7205/140‑1 (MQ=255)                                                                     acgtaacCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCt                                                                    >  1:719653/1‑131 (MQ=255)                                                                     acgtaacCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCt                                                                    <  2:719653/131‑1 (MQ=255)                                                                      cgtaacCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata                                                          >  1:11050/1‑140 (MQ=255)                                                                      cgtaacCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata                                                          >  1:91328/1‑140 (MQ=255)                                                                           cccAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg                                                     >  2:130763/1‑140 (MQ=255)                                                                           cccAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg                                                     >  2:590713/1‑140 (MQ=255)                                                                           cccAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg                                                     >  2:302564/1‑140 (MQ=255)                                                                           cccAATGGGCTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCCAGCCAGATCAGAACCTAGTGACGATTCCCATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg                                                     >  2:917356/1‑140 (MQ=255)                                                                             cAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg                                                   <  2:91328/140‑1 (MQ=255)                                                                             cAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg                                                   <  2:335614/140‑1 (MQ=255)                                                                             cAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg                                                   <  2:12839/140‑1 (MQ=255)                                                                             cAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg                                                   <  2:582754/140‑1 (MQ=255)                                                                             cAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg                                                   <  2:579903/140‑1 (MQ=255)                                                                             cAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg                                                   <  2:801703/140‑1 (MQ=255)                                                                               aTGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGc                                                 <  2:11050/140‑1 (MQ=255)                                                                               aTGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGc                                                 <  1:590713/140‑1 (MQ=255)                                                                               aTGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGc                                                 <  1:982543/140‑1 (MQ=255)                                                                                tGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGc                                                 >  2:63130/1‑139 (MQ=255)                                                                                tGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGc                                                 <  1:63130/139‑1 (MQ=255)                                                                                  ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCttt                                              <  1:302564/140‑1 (MQ=255)                                                                                    tttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTa                                            >  2:424436/1‑140 (MQ=255)                                                                                      tgtgTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGa                                          >  1:513467/1‑140 (MQ=255)                                                                                       gtgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGaa                                         <  1:273071/140‑1 (MQ=255)                                                                                         gtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATa                                       <  2:287273/140‑1 (MQ=255)                                                                                         gtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATa                                       <  1:193728/140‑1 (MQ=255)                                                                                          ttAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGaa                                         >  1:956807/1‑137 (MQ=255)                                                                                          ttAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGaa                                         <  2:956807/137‑1 (MQ=255)                                                                                              cTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGtgtg                                  <  1:424436/140‑1 (MQ=255)                                                                                                  aGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc                              >  2:566351/1‑140 (MQ=255)                                                                                                       tttCAGGCGATGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTg                         >  2:248317/1‑140 (MQ=255)                                                                                                                aTGGTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTaa                >  1:522020/1‑140 (MQ=255)                                                                                                                   gTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAAc             <  1:248317/140‑1 (MQ=255)                                                                                                                   gTCTATCAGAGCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAAc             >  2:360946/1‑140 (MQ=255)                                                                                                                            agCACGCCATATGCACACCAACCGTGCCAGATCAGGACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTAtt    >  1:125591/1‑140 (MQ=255)                                                                                                                             gCACGCCATATGCACACCAACCGTGCCAGATCAGGACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATtg   <  2:125591/140‑1 (MQ=255)                                                                                                                             gCACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATtg   <  2:522020/140‑1 (MQ=255)                                                                                                                              cACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATtgc  >  1:264429/1‑140 (MQ=255)                                                                                                                              cACGCCATATGCACACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATtgc  >  2:696808/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                CTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATATTGTGTAACGTAACCCAATGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGC  >  NC_000913/1720648‑1720912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑