Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,845,130	C→T	V611M (GTG→ATG)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,845,130	0	C	T	100.0%	135.0 / NA	39	V611M (GTG→ATG)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/24);  total (15/24)

CGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCACCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTGCGCGGTCAT  >  NC_000913/1844996‑1845253                                                                                                                                       |                                                                                                                            cGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCg                                                                                                                        <  2:284023/140‑1 (MQ=255) cGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCg                                                                                                                        <  2:673787/140‑1 (MQ=255)      aCGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGt                                                                                                                   <  2:1041724/140‑1 (MQ=255)            tCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCt                                                                                                             >  1:909254/1‑140 (MQ=255)             cGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCtt                                                                                                            <  2:909254/140‑1 (MQ=255)             cGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCtt                                                                                                            <  2:546712/140‑1 (MQ=255)                 ccGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATc                                                                                                        <  2:188386/140‑1 (MQ=255)                  cGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCa                                                                                                       >  2:537151/1‑140 (MQ=255)                   gCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCaa                                                                                                      <  1:5147/140‑1 (MQ=255)                   gCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCaa                                                                                                      <  2:23934/140‑1 (MQ=255)                    cTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCaa                                                                                                      >  2:584856/1‑139 (MQ=255)                    cTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCaa                                                                                                      <  1:584856/139‑1 (MQ=255)                     ttCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATa                                                                                                    <  2:129617/140‑1 (MQ=255)                      tCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATaa                                                                                                   >  1:336153/1‑140 (MQ=255)                      tCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATaa                                                                                                   >  1:943783/1‑140 (MQ=255)                      tCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATaa                                                                                                   <  2:936466/140‑1 (MQ=255)                       ccGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAg                                                                                                  <  1:242118/140‑1 (MQ=255)                             tCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGata                                                                                            <  1:500616/140‑1 (MQ=255)                                     tgGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTcc                                                                                    <  2:798122/140‑1 (MQ=255)                                     tgGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGCACGTTTCGCTTGATCAATAAGCTGATATAGCGTcc                                                                                    >  1:112347/1‑140 (MQ=255)                                      ggCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTccc                                                                                   >  2:303980/1‑140 (MQ=255)                                               tttttCGCACTGCGTTTAGGCGGTGCAGCCTTTTTCTTATCAGGACTGCCACCACTGCCCGGAGCCGCAGTGCCGCGAAACTGCCGCATCGGCGCGCGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCgg                                                                          <  2:112347/140‑1 (MQ=255)                                                     gCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCAt                                                                    <  2:646677/140‑1 (MQ=255)                                                       aCTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATaa                                                                  <  2:943783/140‑1 (MQ=255)                                                                     gTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGa                                                    >  2:1011905/1‑140 (MQ=255)                                                                       gCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGaca                                                  <  1:564400/140‑1 (MQ=255)                                                                                ttCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTc                                         >  1:230111/1‑140 (MQ=255)                                                                                             tGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTc                            >  1:1014621/1‑140 (MQ=255)                                                                                                 accacTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCa                        <  2:336153/140‑1 (MQ=255)                                                                                                 accacTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCa                        <  1:1011905/140‑1 (MQ=255)                                                                                                    acTGCCCGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCg                     >  1:643822/1‑140 (MQ=255)                                                                                                        cccGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTc                 >  1:341548/1‑140 (MQ=255)                                                                                                         ccGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGt                             >  2:461151/1‑127 (MQ=255)                                                                                                         ccGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGt                             <  1:461151/127‑1 (MQ=255)                                                                                                         ccGGAGCCACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTcc                >  2:906112/1‑140 (MQ=255)                                                                                                               ccACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTgc          <  1:537151/140‑1 (MQ=255)                                                                                                               ccACAATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTgc          <  1:281946/140‑1 (MQ=255)                                                                                                                cacaATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTgcg         <  1:906112/140‑1 (MQ=255)                                                                                                                cacaATGCCGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTgcg         <  2:341548/140‑1 (MQ=255)                                                                                                                       ccGCGAAACTGCCGCATCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTGCGCGGTCAt  >  1:250415/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                            CGCTTACGCTATCGCCCCGCTTCCGACTTCATCTGCTGGCGGACTTTTTTTCGCACTACGTTTACGCGGTGCAGCCTTTTTCTTATCAGCACTGCCACCACTGCCCGGAGCCACAATGCCGCGAAACTGCCGCACCGGCGTACGTTTGGCTTGATCAATAAGCTGATATAGCGTCCCCACCAGCGGCTGCATAAAGTCCTGATAGCGACACTGCTTTTCGCTGATTTGCGTCAGCACCGATTCCCAGTGCGCGGTCAT  >  NC_000913/1844996‑1845253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑