Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,884,521	A→G	Y445C (TAC→TGC)	yeaV →	putative transporter YeaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,884,521	0	A	G	100.0%	156.4 / NA	45	Y445C (TAC→TGC)	yeaV	putative transporter YeaV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/22);  total (23/22)

CGCTTTCCATCTTGTTTACCGGTGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTACGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAACCGTATTCAAAGGCGACAACTGAGCGCCATCATCCT  >  NC_000913/1884392‑1884646                                                                                                                                  |                                                                                                                              cGCTTTCCATCTTGTTTACCGGTGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACAtt                                                                                                                     <  2:49576/140‑1 (MQ=255)  gCTTTCCATCTTGTTTACCGGTGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTc                                                                                                                    >  2:769794/1‑140 (MQ=255)   cTTTCCATCTTGTTTACCGGTGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTcc                                                                                                                   >  1:353545/1‑140 (MQ=255)   cTTTCCATCTTGTTTACCGGTGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTcc                                                                                                                   >  2:1028748/1‑140 (MQ=255)           cTTGTTTACCGGTGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGTTGGCTGAAACAGGATTGCGCCGACATTCCGGCtcatc                                                                                                           <  2:926568/140‑1 (MQ=255)              gTTTACCGGTGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAg                                                                                                        <  1:90411/140‑1 (MQ=255)              gTTTACCGGTGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAATCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAg                                                                                                        <  1:898511/140‑1 (MQ=255)                  aCCGGTGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGa                                                                                                    <  1:791161/140‑1 (MQ=255)                      gTGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACAt                                                                                                >  1:251289/1‑140 (MQ=255)                       tGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACAtt                                                                                               >  2:633109/1‑140 (MQ=255)                        gCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGt                                                                                                       <  1:612639/131‑1 (MQ=255)                        gCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGt                                                                                                       >  2:612639/1‑131 (MQ=255)                         cTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTAt                                                                                             <  2:341805/140‑1 (MQ=255)                         cTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTAt                                                                                             >  1:962528/1‑140 (MQ=255)                               tGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCg                                                                                       <  2:190051/140‑1 (MQ=255)                                      gATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGacac                                                                                >  1:437913/1‑140 (MQ=255)                                                       tGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGaa                                                               >  2:440289/1‑140 (MQ=255)                                                           cacaGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGaaaaaa                                                           >  2:898456/1‑140 (MQ=255)                                                              aGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGc                                                        >  2:213346/1‑140 (MQ=255)                                                              aGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGc                                                        <  1:352225/140‑1 (MQ=255)                                                                cccttcccttCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGcta                                                      <  1:769794/140‑3 (MQ=255)                                                                   ttcccttccTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTg                                                   >  2:267412/1‑140 (MQ=255)                                                                   ttcccttccTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTg                                                   >  2:278749/1‑140 (MQ=255)                                                                     cccttccTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCt                                                 >  2:390668/1‑140 (MQ=255)                                                                         tccTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCt                                             <  2:437913/140‑1 (MQ=255)                                                                          ccTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTg                                            >  2:384348/1‑140 (MQ=255)                                                                          ccTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTg                                            >  1:875197/1‑140 (MQ=255)                                                                           cTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGc                                           <  1:633109/140‑1 (MQ=255)                                                                            tCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCg                                          >  1:569125/1‑140 (MQ=255)                                                                             cGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCgg                                         >  2:766085/1‑140 (MQ=255)                                                                             cGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCgg                                         <  1:1028748/140‑1 (MQ=255)                                                                               tCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGGGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGa                                       >  2:987675/1‑140 (MQ=255)                                                                               tCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGa                                       >  1:981470/1‑140 (MQ=255)                                                                                cATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGaa                                      >  1:482106/1‑140 (MQ=255)                                                                                         ggTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAACCGTATTCa                             <  2:482106/140‑1 (MQ=255)                                                                                         ggTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAACCGTATTCa                             <  2:875197/140‑1 (MQ=255)                                                                                          gTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAAc                                     >  1:866529/1‑131 (MQ=255)                                                                                          gTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAAc                                     <  2:866529/131‑1 (MQ=255)                                                                                                     cggGTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAACCGTATTCAAAGGCGACAACt                 <  2:569125/140‑1 (MQ=255)                                                                                                        gTTTATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAACCGTATTCAAAGGCGACAACTGAg              <  1:987675/140‑1 (MQ=255)                                                                                                           tATTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAACCGTATTCAAAGGCGACAACTGAgcgc           <  1:213346/140‑1 (MQ=255)                                                                                                            aTTCGCTGGCTGAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAACCGTATTCAAAGGCGACAACTGAGCGcc          <  2:249963/140‑1 (MQ=255)                                                                                                                  tggctgAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAACCGTATTCAAAGGCGACAACTGAGCGCCatcatc    >  1:464874/1‑140 (MQ=255)                                                                                                                    gctgAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAACCGTATTCAAAGGCGACAACTGAGCGCCATCATCCt  <  1:440289/140‑1 (MQ=255)                                                                                                                    gctgAAACAGGATTGCGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAACCGTATTCAAAGGCGACAACTGAGCGCCATCATCCt  <  1:267412/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                              CGCTTTCCATCTTGTTTACCGGTGCTTCGCTGGAAACGATGAAAACCACCGTCGTGCTCACAGCCCTTCCCTTCCTCGTCATTTTACTGGTGAAAGTCGGCGGGTTTATTCGCTGGCTGAAACAGGATTACGCCGACATTCCGGCTCATCAAGTTGAACATTATCTCCCGCAGACACCGGTTGAAGCCCTGGAAAAAACGCCAGTGCTCCCTGCGGGAACCGTATTCAAAGGCGACAACTGAGCGCCATCATCCT  >  NC_000913/1884392‑1884646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑