Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,954,295	G→A	R285H (CGC→CAC)	cmoB →	tRNA U34 carboxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,954,295	0	G	A	100.0%	211.6 / NA	63	R285H (CGC→CAC)	cmoB	tRNA U34 carboxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/31);  total (32/31)

GGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCC  >  NC_000913/1954159‑1954431                                                                                                                                         |                                                                                                                                         ggCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGacacac                                                                                                                                       <  1:771749/140‑1 (MQ=255) ggCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGacacac                                                                                                                                       >  1:27438/1‑140 (MQ=255) ggCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGacacac                                                                                                                                       <  1:226019/140‑1 (MQ=255)     aTCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGaa                                                                                                                                   >  1:589502/1‑140 (MQ=255)         ttACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAatgga                                                                                                                               <  2:27438/140‑1 (MQ=255)          tACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAatggat                                                                                                                              <  2:650414/140‑1 (MQ=255)                cAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTAc                                                                                                                        >  2:1024535/1‑140 (MQ=255)                             tCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGcc                                                                                                           >  2:509834/1‑140 (MQ=255)                                aTTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGAt                                                                                                        <  2:1004790/140‑1 (MQ=255)                                aTTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGAt                                                                                                        >  1:91943/1‑140 (MQ=255)                                     aTCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCt                                                                                                   <  2:176589/140‑1 (MQ=255)                                     aTCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCt                                                                                                   >  1:586748/1‑140 (MQ=255)                                      tCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTc                                                                                                  >  2:155554/1‑140 (MQ=255)                                        ccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGa                                                                                                <  2:389186/140‑1 (MQ=255)                                        ccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGa                                                                                                <  2:42275/140‑1 (MQ=255)                                        ccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGa                                                                                                <  1:43968/140‑1 (MQ=255)                                        ccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGa                                                                                                <  1:509834/140‑1 (MQ=255)                                           tCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGAccc                                                                                             >  1:440484/1‑140 (MQ=255)                                           tCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGAccc                                                                                             >  1:524566/1‑140 (MQ=255)                                             cgcgCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCcac                                                                                           >  1:436513/1‑140 (MQ=255)                                                  tggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGAt                                                                                      <  2:91943/140‑1 (MQ=255)                                                                             gtgGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCg                                                           <  2:140430/140‑1 (MQ=255)                                                                               ggTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGc                                                         <  2:436513/140‑1 (MQ=255)                                                                                gTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCa                                                        >  2:739240/1‑140 (MQ=255)                                                                                   ttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCgg                                                          >  1:644435/1‑135 (MQ=255)                                                                                   ttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCgg                                                          <  2:644435/135‑1 (MQ=255)                                                                                   ttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCt                                                     >  1:1019176/1‑140 (MQ=255)                                                                                     gTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGa                                                                     >  1:534016/1‑122 (MQ=255)                                                                                     gTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGa                                                                     <  2:534016/122‑1 (MQ=255)                                                                                      tAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTaaa                                                  >  1:634430/1‑140 (MQ=255)                                                                                        gATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcg                                                >  2:68471/1‑140 (MQ=255)                                                                                         atatCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgc                                               <  1:866588/140‑1 (MQ=255)                                                                                         atatCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgc                                               <  2:1019176/140‑1 (MQ=255)                                                                                          tatCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcg                                              >  1:295659/1‑140 (MQ=255)                                                                                          tatCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcg                                              <  1:373342/140‑1 (MQ=255)                                                                                           atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcgc                                             <  2:897396/140‑1 (MQ=255)                                                                                           atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcgc                                             <  2:524566/140‑1 (MQ=255)                                                                                           atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcgc                                             <  1:739240/140‑1 (MQ=255)                                                                                           atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcgc                                             >  2:132644/1‑140 (MQ=255)                                                                                            tCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcgcg                                            <  2:295659/140‑1 (MQ=255)                                                                                             ccGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcg                                              <  2:79012/137‑1 (MQ=255)                                                                                             ccGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcg                                              >  1:79012/1‑137 (MQ=255)                                                                                             ccGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCgg                                           >  2:399640/1‑140 (MQ=255)                                                                                             ccGCATTGCGGATGTGAACGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCgg                                           >  1:876568/1‑140 (MQ=255)                                                                                              cGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGt                                          >  1:669299/1‑140 (MQ=255)                                                                                               gCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTg                                         <  1:68471/140‑1 (MQ=255)                                                                                                     cGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGAtt                                   >  1:671438/1‑140 (MQ=255)                                                                                                       gATGTGAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgc                                 >  1:353929/1‑140 (MQ=255)                                                                                                          gtgAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAACCGCGCGGTGCTGATTgcgcg                              <  2:586748/140‑1 (MQ=255)                                                                                                          gtgAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgcgcg                              <  1:147219/140‑1 (MQ=255)                                                                                                            gAGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCa                            <  1:1017414/140‑1 (MQ=255)                                                                                                             aGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCaa                           >  2:92653/1‑140 (MQ=255)                                                                                                             aGCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCaa                           >  2:133524/1‑140 (MQ=255)                                                                                                              gCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAg                          <  2:51112/140‑1 (MQ=255)                                                                                                              gCGTTACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAg                          <  1:92653/140‑1 (MQ=255)                                                                                                                  tACCACCACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGt                      >  1:943795/1‑140 (MQ=255)                                                                                                                   cccaccaCAGAAGAGCGGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCGGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTa                     <  2:589502/139‑1 (MQ=255)                                                                                                                   accaccACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTa                     >  1:1045556/1‑140 (MQ=255)                                                                                                                   accaccACAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTa                     <  2:440484/140‑1 (MQ=255)                                                                                                                       ccacAGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAgg                 >  1:851500/1‑140 (MQ=255)                                                                                                                        cacaGAAGAGCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGt                <  2:943795/140‑1 (MQ=255)                                                                                                                              agagCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTa          <  1:133524/140‑1 (MQ=255)                                                                                                                               gagCAGCGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGAAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTAt         <  2:1045556/140‑1 (MQ=255)                                                                                                                                  cagcGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTAtt        >  2:1012600/1‑138 (MQ=255)                                                                                                                                  cagcGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTAtt        <  1:1012600/138‑1 (MQ=255)                                                                                                                                  cagcGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTAc      >  2:210458/1‑140 (MQ=255)                                                                                                                                     cGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGc   >  1:982898/1‑140 (MQ=255)                                                                                                                                     cGACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGc   >  1:88484/1‑140 (MQ=255)                                                                                                                                      gACACACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGcc  <  2:876568/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCC  >  NC_000913/1954159‑1954431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑