Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,970,201	T→C	T262A (ACG→GCG)	tap ←	methyl‑accepting chemotaxis protein Tap

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,970,201	0	T	C	100.0%	151.9 / NA	42	T262A (ACG→GCG)	tap	methyl‑accepting chemotaxis protein Tap
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/17);  total (25/17)

TGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGTCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCTACCGATAATGGCCAGTG  >  NC_000913/1970075‑1970335                                                                                                                               |                                                                                                                                       tGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGcc                                                                                                                           <  1:223221/140‑1 (MQ=255)   gcggcCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCctg                                                                                                                         >  1:772748/1‑140 (MQ=255)      gcCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCctgctg                                                                                                                      >  1:444102/1‑140 (MQ=255)       ccGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCtgctgc                                                                                                                     <  1:302331/140‑1 (MQ=255)        cGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCa                                                                                                                    >  1:544440/1‑140 (MQ=255)        cGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCa                                                                                                                    <  1:1037739/140‑1 (MQ=255)        cGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCa                                                                                                                    <  1:192187/140‑1 (MQ=255)                   gCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGa                                                                                                         <  2:59283/140‑1 (MQ=255)                   gCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGa                                                                                                         <  2:544440/140‑1 (MQ=255)                    cGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGAc                                                                                                        >  1:1023856/1‑140 (MQ=255)                                     cGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTg                                                                                       <  2:315141/140‑1 (MQ=255)                                             cTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCAtt                                                                               >  1:640426/1‑140 (MQ=255)                                              ttGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTa                                                                              <  2:640426/140‑1 (MQ=255)                                                gagagaTCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCg                                                                                          >  2:474436/1‑126 (MQ=255)                                                gagagaTCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCg                                                                                          <  1:474436/126‑1 (MQ=255)                                                     aTCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTa                                                                              <  2:852901/133‑1 (MQ=255)                                                     aTCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTa                                                                              >  1:852901/1‑133 (MQ=255)                                                      tCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCata                                                                      >  1:626025/1‑140 (MQ=255)                                                      tCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCata                                                                      <  1:1022669/140‑1 (MQ=255)                                                           aTTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCg                                                                 >  1:209531/1‑140 (MQ=255)                                                               ccTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAAt                                                             >  2:962116/1‑140 (MQ=255)                                                                 tGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCg                                                           >  1:181913/1‑140 (MQ=255)                                                                   cGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGa                                                         >  2:663624/1‑140 (MQ=255)                                                                   cGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGa                                                         >  1:341922/1‑140 (MQ=255)                                                                      cAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGAcgc                                                      <  2:772748/140‑1 (MQ=255)                                                                       aaTCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGAcgcg                                                     <  2:855773/140‑1 (MQ=255)                                                                                            cATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGc                                <  1:962116/140‑1 (MQ=255)                                                                                            cATCTCCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAAACGGACGCGCCAGATTACCCGCCGCAAAGc                                >  2:247978/1‑140 (MQ=255)                                                                                               ctcCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGt                             >  1:487267/1‑140 (MQ=255)                                                                                               ctcCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGt                             >  1:23852/1‑140 (MQ=255)                                                                                                tcCTGGCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTc                            >  1:461669/1‑140 (MQ=255)                                                                                                    ggCTTCCCTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCaaaa                        >  2:46366/1‑140 (MQ=255)                                                                                                         cccTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCt                   >  1:942932/1‑140 (MQ=255)                                                                                                          ccTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAGAAATGGCGGTGTTCCCATTACCACCAATTATCGCGACTGGACACGCCAGATTACCCGCAACCAAGCTCTCAAAATAGCTa                  >  2:727033/1‑140 (MQ=255)                                                                                                          ccTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCTa                  <  1:46366/140‑1 (MQ=255)                                                                                                          ccTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCTa                  >  1:876891/1‑140 (MQ=255)                                                                                                           cTTACGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCTAc                 <  2:181913/140‑1 (MQ=255)                                                                                                               cGCACATCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCTACCGAt             >  1:678345/1‑140 (MQ=255)                                                                                                                    aTCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCTACCGATAATgg        >  2:625894/1‑140 (MQ=255)                                                                                                                    aTCACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGAACATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCTACCGATAATgg        >  2:11208/1‑140 (MQ=255)                                                                                                                      cACTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCTACCGATAATGGcc      <  1:625894/140‑1 (MQ=255)                                                                                                                        cTTACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCTACCGATAAAGGCCAg    >  2:346431/1‑140 (MQ=255)                                                                                                                          tACCGCCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCTACCGATAATGGCCAGTg  <  2:1023856/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                       TGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGATCGTTATTGCCTGCGACAATCTCCGCGATACCAATGTGCATCTCCTGGCTTCCCTTACGCACATCACTTACCGTCCCACGCAAAGCCTGCTGCATGGTCTTCAGACTGGCAAAAATGGCGGTGATCTCATTACGACCATATACCGCAATCGGACGCGCCAGATTACCCGCAGCAATGCTGTCAAAATGGCTACCGATAATGGCCAGTG  >  NC_000913/1970075‑1970335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑