Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,021,330	G→A	L81L (CTG→CTA)	fliN →	flagellar motor switch protein FliN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,021,330	0	G	A	100.0%	275.4 / NA	77	L81L (CTG→CTA)	fliN	flagellar motor switch protein FliN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/37);  total (40/37)

GTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGA  >  NC_000913/2021201‑2021458                                                                                                                                  |                                                                                                                                 gtTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGt                                                                                                                        >  1:963954/1‑140 (MQ=255)  ttCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTc                                                                                                                       <  1:700249/140‑1 (MQ=255)  ttCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTc                                                                                                                       <  1:795205/140‑1 (MQ=255)     cAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTc                                                                                                                       <  1:649449/137‑1 (MQ=255)     cAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTc                                                                                                                       >  2:649449/1‑137 (MQ=255)       gCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCcgtcg                                                                                                                  <  1:822250/140‑1 (MQ=255)          aTTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTgg                                                                                                               >  2:803361/1‑140 (MQ=255)          aTTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTgg                                                                                                               >  2:561798/1‑140 (MQ=255)            ttGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGgcg                                                                                                             >  2:644870/1‑140 (MQ=255)            ttGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATACCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGgcg                                                                                                             >  1:82909/1‑140 (MQ=255)              ggCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCGGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCt                                                                                                           >  2:918633/1‑140 (MQ=255)                cgctggtgATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTgg                                                                                                         >  1:773850/4‑140 (MQ=255)                cGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTgg                                                                                                         <  1:561798/140‑1 (MQ=255)                         tGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTgg                                                                                                >  2:24868/1‑140 (MQ=255)                             aGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTggcggg                                                                                            >  2:762330/1‑140 (MQ=255)                               cGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTggcgggcg                                                                                          <  1:133500/140‑1 (MQ=255)                               cGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTggcgggcg                                                                                          <  1:591377/140‑1 (MQ=255)                                   aCGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAAcc                                                                                      >  2:491654/1‑140 (MQ=255)                                   aCGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAAcc                                                                                      <  1:762330/140‑1 (MQ=255)                                   aCGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAAcc                                                                                      <  1:1005585/140‑1 (MQ=255)                                   aCGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAAcc                                                                                      <  1:644870/140‑1 (MQ=255)                                   aCGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGCACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAAcc                                                                                      <  1:17621/140‑1 (MQ=255)                                    cGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCa                                                                                     <  1:24868/140‑1 (MQ=255)                                    cGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCa                                                                                     <  2:963954/140‑1 (MQ=255)                                           ggATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGata                                                                              >  1:698953/1‑140 (MQ=255)                                            gATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGatat                                                                             >  1:667796/1‑140 (MQ=255)                                                 cGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGa                                                                        >  2:165077/1‑140 (MQ=255)                                                 cGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGa                                                                        >  1:414874/1‑140 (MQ=255)                                                   aCCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTgg                                                                                 >  1:956260/1‑129 (MQ=255)                                                   aCCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATc                                                                      <  1:918633/140‑1 (MQ=255)                                                   aCCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATc                                                                      <  1:289882/140‑1 (MQ=255)                                                   aCCTGATTATCGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTgg                                                                                 <  2:956260/129‑1 (MQ=255)                                                      tGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAAc                                                                   <  1:491654/140‑1 (MQ=255)                                                      tGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAAc                                                                   <  1:635685/140‑1 (MQ=255)                                                      tGATTATGGATATACCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAAc                                                                   <  2:82909/140‑1 (MQ=255)                                                       gATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACg                                                                  >  1:718395/1‑140 (MQ=255)                                                        aTTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACgg                                                                 >  1:928760/1‑140 (MQ=255)                                                                  ttCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATc                                                       >  1:478524/1‑140 (MQ=255)                                                                  ttCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATc                                                       >  1:749265/1‑140 (MQ=255)                                                                  ttCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATc                                                       >  1:745516/1‑140 (MQ=255)                                                                  ttCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATc                                                       >  1:700065/1‑140 (MQ=255)                                                                  ttCCGGCCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATc                                                       >  1:1035048/1‑140 (MQ=255)                                                                   tCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCg                                                      >  1:407507/1‑140 (MQ=255)                                                                     cGGTCCAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGcc                                                    <  2:928760/140‑1 (MQ=255)                                                                     cGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGcc                                                    <  2:718395/140‑1 (MQ=255)                                                                      ggTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGccc                                                   >  2:170528/1‑140 (MQ=255)                                                                       gTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCaa                                                                    >  1:341460/1‑122 (MQ=255)                                                                       gTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCaa                                                                    <  2:341460/122‑1 (MQ=255)                                                                       gTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCa                                                  <  1:165077/140‑1 (MQ=255)                                                                       gTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCa                                                  <  2:698953/140‑1 (MQ=255)                                                                       gTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCa                                                  >  1:404350/1‑140 (MQ=255)                                                                       gTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCa                                                  >  1:668249/1‑140 (MQ=255)                                                                       gTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCa                                                  >  1:253302/1‑140 (MQ=255)                                                                          aaGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAggg                                               >  1:543964/1‑140 (MQ=255)                                                                            gCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCg                                             <  2:1035048/140‑1 (MQ=255)                                                                                 ccGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCg                                             >  2:814115/1‑135 (MQ=255)                                                                                 ccGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCg                                             <  1:814115/135‑1 (MQ=255)                                                                                  cGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGcc                                                    <  2:651312/127‑1 (MQ=255)                                                                                  cGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGcc                                                    >  1:651312/1‑127 (MQ=255)                                                                                        gCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCg                                 <  2:414874/140‑1 (MQ=255)                                                                                         gagacaggTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGt                                <  1:267059/133‑1 (MQ=255)                                                                                          tGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGtt                               >  1:1829/1‑140 (MQ=255)                                                                                           gggCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCGGAGCAACGATTATTTAAGCGCCCAGGGCGAAGAGGTGGTCGGTg                              <  2:773850/140‑1 (MQ=255)                                                                                            ggCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGc                             >  1:842730/1‑140 (MQ=255)                                                                                               cGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGa                          <  2:667796/140‑1 (MQ=255)                                                                                               cGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGa                          <  2:700065/140‑1 (MQ=255)                                                                                               cGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGa                          <  2:745516/140‑1 (MQ=255)                                                                                                 tACGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATa                        >  1:826054/1‑140 (MQ=255)                                                                                                  aCGCGGATGACCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTTATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGACGTTGCCGATaa                       >  2:269545/1‑140 (MQ=255)                                                                                                           aCCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGGGAAACACTGGATATTCTGATCAACGGTTATTTAGTCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGt              >  2:974952/1‑140 (MQ=255)                                                                                                           aCCATCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGGTATTCTGATCAACGGGTATTTAATCGCCCAGGGCGAAGGGGTGGGCGGTGCCGATAAATCTgtctc              >  2:267059/1‑136 (MQ=255)                                                                                                              aTCAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGGGGTGGGCGTTGCCGGTAAATATGGGGTTCg           >  2:1034518/1‑140 (MQ=255)                                                                                                                cAAAGAGCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGGTATTCTGATCAACGGTTATTTAATCGCCCAGGGCGGAGTGGGGGTCGTTGCCGATAAATATGGCGTGCggg         >  2:255509/1‑139 (MQ=255)                                                                                                                   agagCTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCa      <  2:826054/140‑1 (MQ=255)                                                                                                                       cTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGa  <  2:668249/140‑1 (MQ=255)                                                                                                                       cTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGa  <  2:48869/140‑1 (MQ=255)                                                                                                                       cTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGa  <  2:407507/140‑1 (MQ=255)                                                                                                                       cTGTTGCGTCTAACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGa  <  1:1034518/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                 GTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATATCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGTCTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGA  >  NC_000913/2021201‑2021458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑