Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,100,643	T→C	N209S (AAC→AGC)	gnd ←	6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,100,643	0	T	C	100.0%	328.7 / NA	89	N209S (AAC→AGC)	gnd	6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/44);  total (45/44)

CCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACC  >  NC_000913/2100506‑2100773                                                                                                                                          |                                                                                                                                   ccAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTc                                                                                                                                  >  1:654063/1‑140 (MQ=255)     gATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGGAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGc                                                                                                                              >  2:935086/1‑140 (MQ=255)     gATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGc                                                                                                                              <  1:817286/140‑1 (MQ=255)       tCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCa                                                                                                                            <  1:60164/140‑1 (MQ=255)          caTCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCt                                                                                                                         >  1:1039112/1‑140 (MQ=255)          caTCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCt                                                                                                                         >  1:774160/1‑140 (MQ=255)          caTCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCt                                                                                                                         >  1:659364/1‑140 (MQ=255)          caTCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCt                                                                                                                         >  2:174315/1‑140 (MQ=255)          caTCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCt                                                                                                                         >  2:653488/1‑140 (MQ=255)          caTCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCt                                                                                                                         >  1:161728/1‑140 (MQ=255)             cAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTa                                                                                                                      >  1:847487/1‑140 (MQ=255)                    gTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCagag                                                                                                               >  1:621568/1‑140 (MQ=255)                     tAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCa                                                                                                                            <  2:519506/126‑1 (MQ=255)                     tAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCa                                                                                                                            >  1:519506/1‑126 (MQ=255)                          aCCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAgg                                                                                                         <  1:653488/140‑1 (MQ=255)                          aCCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAgg                                                                                                         <  2:161728/140‑1 (MQ=255)                              tCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTc                                                                                                     >  2:899225/1‑140 (MQ=255)                                ttCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCtt                                                                                                      >  2:814587/1‑137 (MQ=255)                                ttCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCtt                                                                                                      <  1:814587/137‑1 (MQ=255)                                        ttttGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGc                                                                                           >  2:327659/1‑140 (MQ=255)                                         tttGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCt                                                                                          <  2:621568/140‑1 (MQ=255)                                            ggTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCa                                                                                       <  1:785306/140‑1 (MQ=255)                                               gAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCt                                                                                          >  1:939159/1‑134 (MQ=255)                                               gAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCt                                                                                          <  2:939159/134‑1 (MQ=255)                                               gAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGACTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCt                                                                                          >  1:303347/1‑134 (MQ=255)                                               gAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGACTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCt                                                                                          <  2:303347/134‑1 (MQ=255)                                                 aGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCg                                                                                  <  1:657928/140‑1 (MQ=255)                                                        tttGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTAtt                                                                           <  1:899225/140‑1 (MQ=255)                                                        tttGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAAGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCg                                                                                  >  1:955397/1‑133 (MQ=255)                                                        tttGGTGATGTCGACCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCATTTCTTCGTTGGTGAGGCTCAAGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCg                                                                                  <  2:955397/133‑1 (MQ=255)                                                           ggTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCaa                                                                        >  2:666299/1‑140 (MQ=255)                                                            gTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAAt                                                                       >  1:862274/1‑140 (MQ=255)                                                             tGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGcc                                                                                <  2:825883/130‑1 (MQ=255)                                                             tGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGcc                                                                                >  1:825883/1‑130 (MQ=255)                                                             tGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATa                                                                      <  2:847487/140‑1 (MQ=255)                                                             tGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATa                                                                      <  2:1039112/140‑1 (MQ=255)                                                              gATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATAc                                                                     >  2:83131/1‑140 (MQ=255)                                                               aTGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATAcc                                                                    >  2:519670/1‑140 (MQ=255)                                                                tGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATa                                                                      >  2:955290/1‑137 (MQ=255)                                                                tGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATa                                                                      <  1:955290/137‑1 (MQ=255)                                                                tGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCg                                                                   >  2:600025/1‑140 (MQ=255)                                                                  tCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTa                                                                             >  1:292866/1‑128 (MQ=255)                                                                  tCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTa                                                                             <  2:292866/128‑1 (MQ=255)                                                                    gATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTtgt                                                               >  2:214826/1‑140 (MQ=255)                                                                      tCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGa                                                             <  1:327659/140‑1 (MQ=255)                                                                        aGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAAc                                                           <  2:862274/140‑1 (MQ=255)                                                                          gTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCa                                                         <  2:659364/140‑1 (MQ=255)                                                                          gTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCa                                                         >  1:244450/1‑140 (MQ=255)                                                                          gTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCa                                                         >  1:29657/1‑140 (MQ=255)                                                                          gTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCa                                                         <  1:159828/140‑1 (MQ=255)                                                                          gTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCa                                                         <  1:600025/140‑1 (MQ=255)                                                                                 cTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTcac                                                  >  1:797049/1‑140 (MQ=255)                                                                                    aGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATa                                               >  1:9323/1‑140 (MQ=255)                                                                                    aGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATa                                               >  1:337734/1‑140 (MQ=255)                                                                                         aCCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGAc                                          >  1:94884/1‑140 (MQ=255)                                                                                           cGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCt                                        >  2:125845/1‑140 (MQ=255)                                                                                                ttCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGcc                                   >  2:734498/1‑140 (MQ=255)                                                                                                ttCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGcc                                   >  2:205307/1‑140 (MQ=255)                                                                                                   cACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATc                                <  2:92063/140‑1 (MQ=255)                                                                                                     cTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCgg                              >  1:350433/1‑140 (MQ=255)                                                                                                      tCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGc                             <  2:337734/140‑1 (MQ=255)                                                                                                      tCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGc                             <  1:778688/140‑1 (MQ=255)                                                                                                      tCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGc                             <  2:94884/140‑1 (MQ=255)                                                                                                       cGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            <  1:935086/140‑1 (MQ=255)                                                                                                       cGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            <  1:188075/140‑1 (MQ=255)                                                                                                       cGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            <  1:214826/140‑1 (MQ=255)                                                                                                       cGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            <  1:519670/140‑1 (MQ=255)                                                                                                       cGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            <  1:83131/140‑1 (MQ=255)                                                                                                       cGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            <  1:125845/140‑1 (MQ=255)                                                                                                       cGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            <  2:147933/140‑1 (MQ=255)                                                                                                       cGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            <  2:9323/140‑1 (MQ=255)                                                                                                       cGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            >  1:147933/1‑140 (MQ=255)                                                                                                       cGGTAAAGGTCTGCGCCAGTTCTTCGCTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            <  1:734498/140‑1 (MQ=255)                                                                                                          tAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            <  1:257572/137‑1 (MQ=255)                                                                                                          tAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCa                            >  2:257572/1‑137 (MQ=255)                                                                                                          tAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCa                         >  1:115126/1‑140 (MQ=255)                                                                                                          tAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCa                         <  2:797049/140‑1 (MQ=255)                                                                                                               gTCTGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAAtata                    >  2:26590/1‑140 (MQ=255)                                                                                                                  tGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGt                 >  2:739846/1‑140 (MQ=255)                                                                                                                  tGCGCCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGt                 <  1:666299/140‑1 (MQ=255)                                                                                                                    cgcCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGc                             >  2:427947/1‑126 (MQ=255)                                                                                                                    cgcCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGc                             <  1:427947/126‑1 (MQ=255)                                                                                                                    cgcCAGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTaa               >  2:173868/1‑140 (MQ=255)                                                                                                                        aGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCa           <  1:173868/140‑1 (MQ=255)                                                                                                                        aGTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCa           <  2:244450/140‑1 (MQ=255)                                                                                                                         gTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAata                      <  2:401944/128‑1 (MQ=255)                                                                                                                         gTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAata                      >  1:401944/1‑128 (MQ=255)                                                                                                                         gTTCTTCGTTGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCAt          >  1:471386/1‑140 (MQ=255)                                                                                                                                 ttGGTGAGGCTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCAcc  <  2:471386/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   CCAGGATCACATCAACCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACC  >  NC_000913/2100506‑2100773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑