Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,124,560	T→C	N396S (AAC→AGC)	wcaI ←	putative colanic biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,124,560	0	T	C	100.0%	106.9 / NA	31	N396S (AAC→AGC)	wcaI	putative colanic biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/15);  total (16/15)

GGGATAAAGTACGCGGGAAAGCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCG  >  NC_000913/2124437‑2124689                                                                                                                            |                                                                                                                                  gggATAAAGTACGCGGGAAAGCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAgcgt                                                                                                                   <  1:575209/140‑1 (MQ=255)    aTAAAGTACGCGGGAAAGCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAgcgtgcg                                                                                                                <  2:770047/140‑1 (MQ=255)      aaaGTACGCGGGAAAGCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGtt                                                                                                              <  2:589954/140‑1 (MQ=255)        aGTACGCGGGAAAGCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCg                                                                                                            <  1:238506/140‑1 (MQ=255)            cgcgGGAAAGCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCat                                                                                                        >  2:246619/1‑140 (MQ=255)             gcgGGAAAGCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCata                                                                                                       >  1:98586/1‑140 (MQ=255)                     gCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTg                                                                                               <  2:422231/140‑1 (MQ=255)                     gCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTg                                                                                               <  1:733868/140‑1 (MQ=255)                           aTAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCg                                                                                         >  2:51007/1‑140 (MQ=255)                            tAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCgt                                                                                        <  2:456843/140‑1 (MQ=255)                              aGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCgtgt                                                                                      <  1:246619/140‑1 (MQ=255)                                                 tGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAg                                                                   <  1:352523/140‑1 (MQ=255)                                                             tGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGAt                                                       >  1:544220/1‑140 (MQ=255)                                                               gATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATCTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATcc                                                     >  2:143540/1‑140 (MQ=255)                                                                aTAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATccc                                                    >  2:891603/1‑140 (MQ=255)                                                                 tAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGa                                                            >  1:824613/1‑131 (MQ=255)                                                                 tAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGa                                                            <  2:824613/131‑1 (MQ=255)                                                                    agTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGcc                                                >  2:760026/1‑140 (MQ=255)                                                                     gTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGcca                                               >  2:599872/1‑140 (MQ=255)                                                                       ttCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGccacc                                             <  2:358062/140‑1 (MQ=255)                                                                         cGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAg                                           >  1:30334/1‑140 (MQ=255)                                                                              gcgcCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCt                                      >  2:821147/1‑140 (MQ=255)                                                                              gcgcCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCt                                      <  1:599872/140‑1 (MQ=255)                                                                              gcgcCATAATTATCCCCGAATATCATCTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCt                                      <  1:143540/140‑1 (MQ=255)                                                                                                aaTATCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCaa                    >  1:376214/1‑140 (MQ=255)                                                                                                 atatCATTTATAAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAAc                   >  1:55747/1‑140 (MQ=255)                                                                                                         tataAATTGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCg           >  1:533251/1‑140 (MQ=255)                                                                                                           taAATTGACGTAACACGCTCTCTTAATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCa         <  1:848982/140‑1 (MQ=255)                                                                                                                tGACGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGcc    >  2:404142/1‑140 (MQ=255)                                                                                                                  aCGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCaa               <  2:614639/127‑1 (MQ=255)                                                                                                                  aCGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCaa               >  1:614639/1‑127 (MQ=255)                                                                                                                  aCGTAACACGCTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCg  >  2:1043579/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                  GGGATAAAGTACGCGGGAAAGCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCG  >  NC_000913/2124437‑2124689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑