Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,199,072	G→A	intergenic (+1774/‑1207)	yehF → / → yehI	putative protein YehF/DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,199,072	0	G	A	100.0%	136.4 / NA	40	intergenic (+1774/‑1207)	yehF/yehI	putative protein YehF/DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/25);  total (15/25)

TCTGTATCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCGTTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCGCTGCTATCGGTAGTGATATCGCCC  >  NC_000913/2198936‑2199198                                                                                                                                         |                                                                                                                               tctGTATCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCCGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATtc                                                                                                                             >  2:229850/1‑140 (MQ=255)  ctGTATCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATtct                                                                                                                            <  2:782609/140‑1 (MQ=255)    gTATCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCg                                                                                                                          <  2:211280/140‑1 (MQ=255)      aTCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGt                                                                                                                        <  1:927050/140‑1 (MQ=255)          gggATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACg                                                                                                                    >  1:686152/1‑140 (MQ=255)               tattGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGac                                                                                                               >  1:705124/1‑140 (MQ=255)                 ttGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGacac                                                                                                             <  1:580998/140‑1 (MQ=255)                      ggTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACAccgccc                                                                                                        >  1:95742/1‑140 (MQ=255)                               cGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGa                                                                                               <  1:748500/140‑1 (MQ=255)                                 tCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGTGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACg                                                                                             >  1:629955/1‑140 (MQ=255)                                 tCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACg                                                                                             <  2:705124/140‑1 (MQ=255)                                     ttCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCAt                                                                                         <  1:569465/140‑1 (MQ=255)                                          ccgACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATa                                                                                    <  1:259418/140‑1 (MQ=255)                                               tCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGTCGCCATTAATACgcgc                                                                               <  2:910449/140‑1 (MQ=255)                                               tCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACgcgc                                                                               >  1:77477/1‑140 (MQ=255)                                                         gATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGt                                                                     >  2:252568/1‑140 (MQ=255)                                                             tGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAAt                                                                 <  1:252568/140‑1 (MQ=255)                                                                   ggATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGc                                                           >  2:196391/1‑140 (MQ=255)                                                                         gTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCAt                                                         <  1:485687/136‑1 (MQ=255)                                                                         gTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCAt                                                         >  2:485687/1‑136 (MQ=255)                                                                             aCCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACgcgc                                                 <  2:240410/140‑1 (MQ=255)                                                                             aCCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACgcgc                                                 >  2:796685/1‑140 (MQ=255)                                                                               cGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCa                                               >  1:520438/1‑140 (MQ=255)                                                                                    ggCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCgtt                                          <  2:990983/140‑1 (MQ=255)                                                                                    ggCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCgtt                                          <  2:520438/140‑1 (MQ=255)                                                                                       aGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCgttggg                                       <  1:351876/140‑1 (MQ=255)                                                                                           cGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCgttgggttgg                                   >  1:934438/1‑140 (MQ=255)                                                                                               ggCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGatat                               <  1:229850/140‑1 (MQ=255)                                                                                                    gcCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCg                          <  1:796685/140‑1 (MQ=255)                                                                                                     ccGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGa                                  <  1:744643/131‑1 (MQ=255)                                                                                                     ccGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGa                                  >  2:744643/1‑131 (MQ=255)                                                                                                        tCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCgctgc                      <  2:629955/140‑1 (MQ=255)                                                                                                        tCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCg                          <  2:1019459/136‑1 (MQ=255)                                                                                                        tCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCg                          >  1:1019459/1‑136 (MQ=255)                                                                                                         cGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCgctgct                     <  2:5260/140‑1 (MQ=255)                                                                                                           aaaGAGAGTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCGCTGCTAt                   <  2:673778/140‑1 (MQ=255)                                                                                                                  gTTGGGCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCGCTGCTATCGGTAGt            >  1:343967/1‑140 (MQ=255)                                                                                                                       gCGTTCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCGCTGCTATCGGTAGTGatat       <  2:77477/140‑1 (MQ=255)                                                                                                                           tCACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCGCTGCTATCGGTAGTGATATCGcc   <  1:196391/140‑1 (MQ=255)                                                                                                                            cACTGCGTTAGGCATTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCTCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCGCTGCTATCGGTAGTGATATCGccc  <  2:937523/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                               TCTGTATCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCTGTTTACCGCCTGGCAGACCGCTGGCGCGCCGTCGAAAGAGAGTTGGGCGTTCACTGCGTTAGGCGTTCTCGGTAACGATGACACCGCCCGCAAACTGACGCCATTAATACGCGCCTGGCCTGGTGAATCCCAGCATAAACGCGCCACCGTTGGGTTGGATATTCTCGCTGCTATCGGTAGTGATATCGCCC  >  NC_000913/2198936‑2199198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑