Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,569,455	T→C	Q19Q (CAA→CAG)	eutG ←	putative alcohol dehydrogenase in ethanolamine utilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,569,455	0	T	C	100.0%	209.2 / NA	57	Q19Q (CAA→CAG)	eutG	putative alcohol dehydrogenase in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/31);  total (26/31)

TCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGTCATAAACAAACTGTGCTGCGGTAAA  >  NC_000913/2569319‑2569585                                                                                                                                         |                                                                                                                                   tCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGc                                                                                                                                 <  1:948131/140‑1 (MQ=255)            tGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGt                                                                                                                      >  1:998684/1‑140 (MQ=255)            tGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGt                                                                                                                      >  1:471189/1‑140 (MQ=255)                 aaaGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGa                                                                                                                 >  1:574092/1‑140 (MQ=255)                 aaaGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGa                                                                                                                 <  2:471189/140‑1 (MQ=255)                      tGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGcc                                                                                                            <  2:977417/140‑1 (MQ=255)                             cATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAaga                                                                                                     <  2:776934/140‑1 (MQ=255)                                cACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAgcg                                                                                                  <  2:733329/140‑1 (MQ=255)                                  cGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTATCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGcgcg                                                                                                >  2:144542/1‑140 (MQ=255)                                       aGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTg                                                                                           >  2:15878/1‑140 (MQ=255)                                         aTGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCa                                                                                         >  2:156132/1‑140 (MQ=255)                                              tCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCa                                                                                    >  2:116400/1‑140 (MQ=255)                                                aGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAtt                                                                                  <  1:528843/140‑1 (MQ=255)                                                 gCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAttt                                                                                 >  1:137082/1‑140 (MQ=255)                                                 gCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAttt                                                                                 <  2:574092/140‑1 (MQ=255)                                                 gCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAttt                                                                                 >  2:622179/1‑140 (MQ=255)                                                 gCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAttt                                                                                 >  1:759688/1‑140 (MQ=255)                                                 gCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAttt                                                                                 >  2:1008933/1‑140 (MQ=255)                                                  cccACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAtttt                                                                                <  1:940571/140‑1 (MQ=255)                                                   ccACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTg                                                                               <  1:156132/140‑1 (MQ=255)                                                     aCGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCa                                                                             >  2:905530/1‑140 (MQ=255)                                                       gcgTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAtt                                                                                  <  2:920218/133‑1 (MQ=255)                                                       gcgTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAtt                                                                                  >  1:920218/1‑133 (MQ=255)                                                        cgTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCatat                                                                          <  1:905530/140‑1 (MQ=255)                                                         gTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCAtata                                                                         <  1:406695/140‑1 (MQ=255)                                                                 ttGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAttt                                                                                 <  2:801390/124‑1 (MQ=255)                                                                 ttGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAttt                                                                                 >  1:801390/1‑124 (MQ=255)                                                                    cTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCt                                                              <  2:998684/140‑1 (MQ=255)                                                                         cGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTtctc                                                         <  1:1024328/140‑1 (MQ=255)                                                                              cTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGcc                                                    <  1:90307/140‑1 (MQ=255)                                                                              cTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGcc                                                    <  2:759688/140‑1 (MQ=255)                                                                                gCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCa                                                  <  1:11542/140‑1 (MQ=255)                                                                                 cTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCg                                                      <  1:587530/135‑1 (MQ=255)                                                                                 cTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCg                                                      >  2:587530/1‑135 (MQ=255)                                                                                 cTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCac                                                 >  1:898620/1‑140 (MQ=255)                                                                                 cTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCac                                                 >  1:339254/1‑140 (MQ=255)                                                                                 cTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCac                                                 >  1:636710/1‑140 (MQ=255)                                                                                   cACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCacta                                               >  2:581655/1‑140 (MQ=255)                                                                                       gAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTgg                                           <  2:137082/140‑1 (MQ=255)                                                                                         gCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCg                                         >  1:455784/1‑140 (MQ=255)                                                                                            cGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATc                                      <  1:581655/140‑1 (MQ=255)                                                                                                   cAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCg                               >  2:276544/1‑140 (MQ=255)                                                                                                   cAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCg                               <  1:145331/140‑1 (MQ=255)                                                                                                   cAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCg                               <  2:636710/140‑1 (MQ=255)                                                                                                     aaGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCggg                             <  2:455784/140‑1 (MQ=255)                                                                                                       gCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGt                           <  1:1008933/140‑1 (MQ=255)                                                                                                       gCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGt                           <  2:20652/140‑1 (MQ=255)                                                                                                       gCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGt                           <  1:116400/140‑1 (MQ=255)                                                                                                       gCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGt                           <  1:23624/140‑1 (MQ=255)                                                                                                       gCGTCACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGt                           <  1:249305/140‑1 (MQ=255)                                                                                                           cACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCg                               >  1:223145/1‑132 (MQ=255)                                                                                                           cACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCg                               <  2:223145/132‑1 (MQ=255)                                                                                                           cACCGGTGGAACGCTAAATGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGTCATa                       >  1:520652/1‑140 (MQ=255)                                                                                                                           aaTGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGTCATAAACAAACTGTGCTGCg       >  2:847817/1‑140 (MQ=255)                                                                                                                           aaTGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGTCATAAACAAACTGTGCTGCg       >  2:193281/1‑140 (MQ=255)                                                                                                                             tGTTTTTACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGTCATAAACAAACTGTGCTGCGGt     <  2:898620/140‑1 (MQ=255)                                                                                                                                ttttACCCGCTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGTCATAAACAAACTGTGCTGCGGTaaa  >  2:538776/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   TCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGTCATAAACAAACTGTGCTGCGGTAAA  >  NC_000913/2569319‑2569585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑