Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,596,151	T→C	N196S (AAC→AGC)	ypfJ ←	uncharacterized protein YpfJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,596,151	0	T	C	100.0%	205.7 / NA	57	N196S (AAC→AGC)	ypfJ	uncharacterized protein YpfJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/28);  total (29/28)

CTGCGCCGCGTTCAGCGCCTCTTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGTTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATCCGCGCCAAGTTTGT  >  NC_000913/2596030‑2596286                                                                                                                          |                                                                                                                                        cTGCGCCGCGTTCAGCGCCTCTTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTcg                                                                                                                       >  1:805050/1‑140 (MQ=255) cTGCGCCGCGTTCAGCGCCTCTTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTcg                                                                                                                       <  2:108954/140‑1 (MQ=255) cTGCGCCGCGTTCAGCGCCTCTTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTcg                                                                                                                       <  2:835961/140‑1 (MQ=255)  tGCGCCGCGTTCAGCGCCTCTTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTcgc                                                                                                                      >  2:514147/1‑140 (MQ=255)    cgccgcGTTCAGCGCCTCTTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGt                                                                                                                    >  2:482760/1‑140 (MQ=255)    cgccgcGTTCAGCGCCTCTTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGt                                                                                                                    >  1:46005/1‑140 (MQ=255)     gccgcGTTCAGCGCCTCTTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGtt                                                                                                                   <  1:60038/140‑1 (MQ=255)         cgTTCAGCGCCTCTTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTttgt                                                                                                               >  2:894401/1‑140 (MQ=255)                    tctTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACg                                                                                                    <  2:805050/140‑1 (MQ=255)                    tctTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACg                                                                                                    <  1:894401/140‑1 (MQ=255)                    tctTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACg                                                                                                    <  1:400231/140‑1 (MQ=255)                     ctTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGa                                                                                                   >  2:491525/1‑140 (MQ=255)                      ttCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGaa                                                                                                  >  2:416033/1‑140 (MQ=255)                             tCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCgg                                                                                           >  1:349334/1‑140 (MQ=255)                               gCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCt                                                                                         <  2:46005/140‑1 (MQ=255)                               gCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCt                                                                                         <  2:501388/140‑1 (MQ=255)                                           aaCGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACa                                                                             <  2:296593/140‑1 (MQ=255)                                               ccTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGttt                                                                         <  2:637344/140‑1 (MQ=255)                                                  tgctgctgcATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTg                                                                      <  1:514147/140‑1 (MQ=255)                                                    ctgctgcATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGca                                                                    <  1:371340/140‑1 (MQ=255)                                                          cATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACatgat                                                              >  2:245122/1‑140 (MQ=255)                                                            tACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGttt                                                                         >  2:685483/1‑127 (MQ=255)                                                            tACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGttt                                                                         <  1:685483/127‑1 (MQ=255)                                                              cTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGAcc                                                          <  1:416033/140‑1 (MQ=255)                                                                 tGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGAc                                                       >  2:136642/1‑140 (MQ=255)                                                                 tGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGAc                                                       >  1:288913/1‑140 (MQ=255)                                                                    ccccAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTc                                                    >  1:85779/1‑140 (MQ=255)                                                                      ccAGACACCGGCAAAACAGTCGGCTTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCAt                                                  >  2:568387/1‑140 (MQ=255)                                                                      ccAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATGCGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCAt                                                  >  1:367076/1‑140 (MQ=255)                                                                      ccAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCa                                                   <  1:858719/139‑1 (MQ=255)                                                                      ccAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCa                                                   >  2:858719/1‑139 (MQ=255)                                                                      ccAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCAt                                                  >  1:615689/1‑140 (MQ=255)                                                                       cAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACGGTTTCTGCACATGATGACCGACTTCATg                                                 >  2:702749/1‑140 (MQ=255)                                                                        aGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATgg                                                >  2:533643/1‑140 (MQ=255)                                                                         gACACCGGCAAAACAGTCGGCCTGGAGTTCCATGCGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATggg                                               <  2:367076/140‑1 (MQ=255)                                                                         gACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATggg                                               <  1:136642/140‑1 (MQ=255)                                                                         gACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATggg                                               <  1:1007804/140‑1 (MQ=255)                                                                         gACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATggg                                               <  1:482760/140‑1 (MQ=255)                                                                         gACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATggg                                               >  1:501163/1‑140 (MQ=255)                                                                         gACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATggg                                               <  2:615689/140‑1 (MQ=255)                                                                           cacCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCg                                             <  2:349334/140‑1 (MQ=255)                                                                            acCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGa                                            <  2:963172/140‑1 (MQ=255)                                                                            acCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGa                                            <  1:750041/140‑1 (MQ=255)                                                                                gCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAAc                                        >  2:374150/1‑140 (MQ=255)                                                                                         tCGGCCTGGAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTg                               >  1:794093/1‑140 (MQ=255)                                                                                                 gAGTTCCATACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAAt                       >  1:991176/1‑140 (MQ=255)                                                                                                        aTACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATc                <  2:85779/140‑1 (MQ=255)                                                                                                         tACGCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATcc               >  2:469337/1‑140 (MQ=255)                                                                                                            gCACAGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATCcgcg            >  2:452128/1‑140 (MQ=255)                                                                                                              acaGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATCCGCGcc          >  2:816270/1‑140 (MQ=255)                                                                                                              acaGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATCCGCGcc          >  1:222692/1‑140 (MQ=255)                                                                                                                aGATAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATCCGCGCCaa        >  1:740147/1‑140 (MQ=255)                                                                                                                   tAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATCCGCGCCAAGtt     <  1:568387/140‑1 (MQ=255)                                                                                                                   tAAGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATCCGCGCCAAGtt     <  1:245122/140‑1 (MQ=255)                                                                                                                     aGCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATCCGCGCCAAGTTTg   >  1:93020/1‑140 (MQ=255)                                                                                                                      gCGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATCCGCGCCAAGTTTGt  <  1:452128/140‑1 (MQ=255)                                                                                                                       cGGCTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATCCgcgc           <  1:788538/130‑1 (MQ=255)                                                                                                                          |                                                                                                                                        CTGCGCCGCGTTCAGCGCCTCTTCCAGATCGCCGGTTTCCAGAACGCCTTGCTGCTGCATACTATGCCCCCAGACACCGGCAAAACAGTCGGCCTGGAGTTCCATACGCACAGATAAGCGGTTTACTTCCGCCTGCGTCGCGTTTTGTTGCAGTTGACGAACTTTCGGCTCGATGCCTAACAGTTTCTGCACATGATGACCGACTTCATGGGCGATAACGTACCCCTGGGCAAAATCGCCATCCGCGCCAAGTTTGT  >  NC_000913/2596030‑2596286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑