Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,617,121	C→T	A343V (GCA→GTA)	bepA →	beta‑barrel assembly‑enhancing protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,617,121	0	C	T	100.0%	196.5 / NA	57	A343V (GCA→GTA)	bepA	beta‑barrel assembly‑enhancing protease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/26);  total (31/26)

GGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGCATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCTCAACCTGGCGAACGCTTATTTGCAA  >  NC_000913/2616984‑2617257                                                                                                                                          |                                                                                                                                         ggATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTAt                                                                                                                                        <  2:951300/140‑1 (MQ=255)  gATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATg                                                                                                                                       <  2:52422/140‑1 (MQ=255)    tGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGt                                                                                                                                     >  1:957167/1‑140 (MQ=255)       aTGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTAtc                                                                                                                                  >  1:78239/1‑140 (MQ=255)       aTGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTAtc                                                                                                                                  <  2:78239/140‑1 (MQ=255)         gggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGCCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGCAtctc                                                                                                                                >  1:305679/1‑140 (MQ=255)              aaaGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCt                                                                                                                           >  2:111415/1‑140 (MQ=255)              aaaGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCt                                                                                                                           <  2:957167/140‑1 (MQ=255)               aaGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTg                                                                                                                          <  1:501309/140‑1 (MQ=255)                aGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGAt                                                                                                                             >  2:746676/1‑136 (MQ=255)                aGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGAt                                                                                                                             <  1:746676/136‑1 (MQ=255)                   aaaCGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTa                                                                                                                      <  2:691804/140‑1 (MQ=255)                    aaCGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTAc                                                                                                                     <  1:596224/140‑1 (MQ=255)                     aCGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACt                                                                                                                    >  2:466535/1‑140 (MQ=255)                                     agcagcACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGc                                                                                                    <  2:134835/140‑1 (MQ=255)                                          cacaATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGGCGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTGTGGTATCTCGATCTGGCTACTGATATCGATc                                                                                                          >  2:980664/1‑129 (MQ=255)                                          cacaATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATc                                                                                                          <  1:980664/129‑1 (MQ=255)                                          cacaATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAAc                                                                                               >  2:208004/1‑140 (MQ=255)                                            caATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACaa                                                                                             <  1:522002/140‑1 (MQ=255)                                                    tCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATg                                                                                     >  1:555686/1‑140 (MQ=255)                                                    tCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATg                                                                                     >  1:904522/1‑140 (MQ=255)                                                    tCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGAACTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATg                                                                                     <  1:443336/140‑1 (MQ=255)                                                      gTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAg                                                                                   <  2:151822/140‑1 (MQ=255)                                                         cTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAg                                                                                   >  2:985981/1‑137 (MQ=255)                                                         cTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAg                                                                                   <  1:985981/137‑1 (MQ=255)                                                         cTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCg                                                                                >  1:90994/1‑140 (MQ=255)                                                               aGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAAt                                                                          <  2:90994/140‑1 (MQ=255)                                                                      ggAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGa                                                                   >  2:763620/1‑140 (MQ=255)                                                                      ggAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGa                                                                   >  1:539940/1‑140 (MQ=255)                                                                              ataaataCGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGcc                                                           >  2:708616/1‑140 (MQ=255)                                                                                  ataCGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCcgcg                                                       <  2:904522/140‑1 (MQ=255)                                                                                       acgaGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTa                                                  <  1:763620/140‑1 (MQ=255)                                                                                          aGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCcgcg                                                       >  2:718151/1‑132 (MQ=255)                                                                                          aGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCcgcg                                                       <  1:718151/132‑1 (MQ=255)                                                                                          aGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGc                                               >  1:123080/1‑140 (MQ=255)                                                                                           ggCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCa                                              >  1:232948/1‑140 (MQ=255)                                                                                           ggCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCa                                              >  1:748683/1‑140 (MQ=255)                                                                                           ggCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCa                                              >  1:719246/1‑140 (MQ=255)                                                                                           ggCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCa                                              >  1:66529/1‑140 (MQ=255)                                                                                             cgcgTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCAcc                                            >  1:896744/1‑140 (MQ=255)                                                                                                    aaCGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCgg                                     <  1:194533/140‑1 (MQ=255)                                                                                                         tGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTg                                >  2:45959/1‑140 (MQ=255)                                                                                                         tGCAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTg                                >  1:878637/1‑140 (MQ=255)                                                                                                           cAACCGTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCa                              >  1:893401/1‑140 (MQ=255)                                                                                                                gTTACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCTCa                         >  2:585389/1‑140 (MQ=255)                                                                                                                 ttACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCt                           <  2:353771/137‑1 (MQ=255)                                                                                                                 ttACTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCt                           >  1:353771/1‑137 (MQ=255)                                                                                                                   aCTGGCGGCAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCTCAAcc                      >  1:95907/1‑140 (MQ=255)                                                                                                                      ggcggcAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCTCAACCTgg                   <  1:208004/140‑1 (MQ=255)                                                                                                                       gcggcAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCTCAACCTGGc                  >  2:467797/1‑140 (MQ=255)                                                                                                                        cggcAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCTCAACCTGGCg                 >  2:652888/1‑140 (MQ=255)                                                                                                                          gcAGAACCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCTCAACCTGGCGaa               <  2:123080/140‑1 (MQ=255)                                                                                                                              aaCCTGGCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCTCAACCTGGCGAACGCt           <  2:896744/140‑1 (MQ=255)                                                                                                                                   ggCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGA‑TTACGCACCAATCCGGTGTTGCAGCTCAACCTGGCGaa               <  2:856196/130‑1 (MQ=255)                                                                                                                                   ggCAACGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGA‑TTACGCACCAATCCGGTGTTGCAGCTCAACCTGGCGaa               >  1:856196/1‑130 (MQ=255)                                                                                                                                      aaCGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCTCAACCTGGCGAACGCTTATTTGCa   <  1:652888/140‑1 (MQ=255)                                                                                                                                       aCGTATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCTCAACCTGGCGAACGCTTATTTGCaa  <  1:708616/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAAAACGCTGCAACCGTTACTGGCGGCAGAACCTGGCAACGCATGGTATCTCGATCTGGCTACTGATATCGATCTTGGGCAAAACAAAGCCAATGAGGCGATCAATCGTCTGAAAAATGCCCGCGATTTACGCACCAATCCGGTGTTGCAGCTCAACCTGGCGAACGCTTATTTGCAA  >  NC_000913/2616984‑2617257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑