Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,656,431	G→A	intergenic (‑73/+105)	pepB ← / ← iscX	aminopeptidase B/accessory iron‑sulfur cluster assembly protein IscX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,656,431	0	G	A	100.0%	154.8 / NA	44	intergenic (‑73/+105)	pepB/iscX	aminopeptidase B/accessory iron‑sulfur cluster assembly protein IscX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/25);  total (19/25)

GCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCGTCCAGCCAGACTAACAAAA  >  NC_000913/2656294‑2656567                                                                                                                                          |                                                                                                                                         gCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACa                                                                                                                                        >  1:41608/1‑140 (MQ=255)                     gTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAgtgagtga                                                                                                                    >  1:460191/1‑140 (MQ=255)                          cAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGccc                                                                                                               <  2:460191/140‑1 (MQ=255)                          cAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGccc                                                                                                               <  2:304410/140‑1 (MQ=255)                           aGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGcccc                                                                                                              <  1:375331/140‑1 (MQ=255)                               tGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGa                                                                                                          <  1:941775/140‑1 (MQ=255)                               tGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGa                                                                                                          <  2:916164/140‑1 (MQ=255)                               tGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGa                                                                                                          <  2:243191/140‑1 (MQ=255)                                gggTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAg                                                                                                         <  2:175005/140‑1 (MQ=255)                                 ggTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGc                                                                                                        <  2:505484/140‑1 (MQ=255)                                 ggTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGc                                                                                                        <  2:382667/140‑1 (MQ=255)                                 ggTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGc                                                                                                        >  1:539154/1‑140 (MQ=255)                                      agagGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGa                                                                                                   >  1:357453/1‑140 (MQ=255)                                       gagGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGAt                                                                                                  <  2:393861/140‑1 (MQ=255)                                       gagGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGAt                                                                                                  <  2:209408/140‑1 (MQ=255)                                            tAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTa                                                                                             >  1:98964/1‑140 (MQ=255)                                                cTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTAtgtg                                                                                         >  2:386732/1‑140 (MQ=255)                                                 ttCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGa                                                                                        >  2:257141/1‑140 (MQ=255)                                                  tCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGAc                                                                                       <  1:1007646/140‑1 (MQ=255)                                                    aTCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTg                                                                                     >  1:368265/1‑140 (MQ=255)                                                                  tttAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAgcgc                                                                       >  1:569515/1‑140 (MQ=255)                                                                   ttAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACg                                                                            >  2:105089/1‑134 (MQ=255)                                                                   ttAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACg                                                                            <  1:105089/134‑1 (MQ=255)                                                                     aGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGc                                                                    <  1:386732/140‑1 (MQ=255)                                                                             ttcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCAt                                                            >  2:280020/1‑140 (MQ=255)                                                                                  tGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTgcg                                                       <  2:449368/140‑1 (MQ=255)                                                                                    tAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTgcggc                                                     >  2:31347/1‑140 (MQ=255)                                                                                    tAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTgcggc                                                     >  1:370965/1‑140 (MQ=255)                                                                                     aaaaGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTgcggcg                                                    <  1:31347/140‑1 (MQ=255)                                                                                          ggCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTgcggc                                                     <  1:660856/134‑1 (MQ=255)                                                                                          ggCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTgcggc                                                     >  2:660856/1‑134 (MQ=255)                                                                                            ccgccAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCa                                             >  1:949823/1‑140 (MQ=255)                                                                                                  gTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGAcgc                                       <  1:428725/140‑1 (MQ=255)                                                                                                            ccAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAattatt                             <  2:949823/140‑1 (MQ=255)                                                                                                             cAGGATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTc                            >  1:259861/1‑140 (MQ=255)                                                                                                                gATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCgac                         <  2:370965/140‑3 (MQ=255)                                                                                                                gATTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGc                         <  1:257141/140‑1 (MQ=255)                                                                                                                 aTTTTTCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGcc                        <  2:98964/140‑1 (MQ=255)                                                                                                                    tttCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCg                     <  2:569515/140‑1 (MQ=255)                                                                                                                    tttCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCg                     >  1:120531/1‑140 (MQ=255)                                                                                                                    tttCCGCTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCg                     <  1:54525/140‑1 (MQ=255)                                                                                                                          cTAAATGATTCGCGTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCGTccagc               >  1:746547/1‑140 (MQ=255)                                                                                                                                    cgcgTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCGTCCAGCCAGACTAACa     >  1:458316/1‑140 (MQ=255)                                                                                                                                       gTTACAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCGTCCAGCCAGACTAACaaaa  <  2:746547/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCGTCCAGCCAGACTAACAAAA  >  NC_000913/2656294‑2656567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑