Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	313,061	A→G	intergenic (‑284/+80)	ykgM ← / ← ykgR	putative ribosomal protein/putative membrane protein YkgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	313,061	0	A	G	100.0%	213.6 / NA	59	intergenic (‑284/+80)	ykgM/ykgR	putative ribosomal protein/putative membrane protein YkgR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/32);  total (27/32)

TAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAAC  >  NC_000913/312926‑313194                                                                                                                                        |                                                                                                                                      tAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGa                                                                                                                                   <  2:200693/140‑1 (MQ=255)        cTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATataata                                                                                                                            >  2:748626/1‑140 (MQ=255)        cTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATataata                                                                                                                            >  2:672164/1‑140 (MQ=255)          ggATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGT‑TTCCTCTCCCGCGGATATAATAAtt                                                                                                                         >  1:506733/1‑140 (MQ=255)               aGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAAttactt                                                                                                                     <  2:313/140‑1 (MQ=255)               aGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAAttactt                                                                                                                     <  2:557475/140‑1 (MQ=255)                     tgatgaTGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACaaaa                                                                                                               <  1:990876/140‑1 (MQ=255)                      gatgatGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACaaaaa                                                                                                              >  1:23088/1‑140 (MQ=255)                         gatgGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAgg                                                                                                           >  2:545453/1‑140 (MQ=255)                           tgGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCAGATATAATAATTACTTACAAAAAAGGga                                                                                                         <  1:725702/140‑1 (MQ=255)                           tgGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCAGATATAATAATTACTTACAAAAAAGGga                                                                                                         >  2:725702/1‑140 (MQ=255)                           tgGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCACGCGGATATAATAATTACTTACAAAAAAGGga                                                                                                         >  2:727093/1‑140 (MQ=255)                             gTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGgaga                                                                                                       >  1:764060/1‑140 (MQ=255)                             gTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGgaga                                                                                                       >  1:712031/1‑140 (MQ=255)                               tttGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAgg                                                                                                     <  1:629102/140‑1 (MQ=255)                                  gCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATg                                                                                                  <  2:473451/140‑1 (MQ=255)                                    tGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCa                                                                                                <  1:617646/140‑1 (MQ=255)                                      agagTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCata                                                                                              <  1:672164/140‑1 (MQ=255)                                             ggATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTaaa                                                                                       <  2:50279/140‑1 (MQ=255)                                             ggATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTaaa                                                                                       <  1:60241/140‑1 (MQ=255)                                                  tttCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCa                                                                                  >  1:878187/1‑140 (MQ=255)                                                  tttCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCa                                                                                  >  1:220597/1‑140 (MQ=255)                                                  tttCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCa                                                                                  >  1:54843/1‑140 (MQ=255)                                                  tttCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCACGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCa                                                                                  <  1:727093/140‑1 (MQ=255)                                                    tCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACt                                                                                >  2:490685/1‑140 (MQ=255)                                                       agaATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGaa                                                                             <  2:801701/140‑1 (MQ=255)                                                            ggAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGaa                                                                        <  2:640002/140‑1 (MQ=255)                                                            ggAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGaa                                                                        <  1:545453/140‑1 (MQ=255)                                                            ggAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGaa                                                                        <  2:805707/140‑1 (MQ=255)                                                            ggAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTACTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCGCTGAAGTGaa                                                                        <  2:911908/140‑1 (MQ=255)                                                                gTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGt                                                                    >  2:124025/1‑140 (MQ=255)                                                                 ttATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGtt                                                                   <  2:131428/140‑1 (MQ=255)                                                                        cACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTcc                                                            <  1:834883/140‑1 (MQ=255)                                                                          cTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGt                                                          >  1:394663/1‑140 (MQ=255)                                                                               cTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGttatta                                                     >  2:185411/1‑140 (MQ=255)                                                                                tGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTaa                                                    <  1:490685/140‑1 (MQ=255)                                                                                tGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATCTCCGTTATTaa                                                    <  1:316648/140‑1 (MQ=255)                                                                                  gCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTaa                                                    <  2:755381/138‑1 (MQ=255)                                                                                  gCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTaa                                                    >  1:755381/1‑138 (MQ=255)                                                                                        aaTTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATg                                            <  1:748626/140‑1 (MQ=255)                                                                                             aTCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGaa                                       <  1:924070/140‑1 (MQ=255)                                                                                                     aTGATTGATTACCTGACTGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTTAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAg                               <  2:935164/140‑1 (MQ=255)                                                                                                     aTGATTGATTACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAg                               <  1:185411/140‑1 (MQ=255)                                                                                                        attgattACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCg                            <  2:220597/140‑1 (MQ=255)                                                                                                        attgattACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCg                            <  1:620629/140‑1 (MQ=255)                                                                                                              tACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCt                      >  2:832793/1‑140 (MQ=255)                                                                                                              tACCTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCt                      >  1:477161/1‑140 (MQ=255)                                                                                                                 cTGACAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACa                   >  1:1032628/1‑140 (MQ=255)                                                                                                                    aCAGCGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAAtt                >  2:367863/1‑140 (MQ=255)                                                                                                                        cGTGTATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGa            >  1:144105/1‑140 (MQ=255)                                                                                                                          tgtATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACa          >  1:59634/1‑140 (MQ=255)                                                                                                                          tgtATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACa          >  2:65679/1‑140 (MQ=255)                                                                                                                          tgtATTCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACa          <  2:59634/140‑1 (MQ=255)                                                                                                                              ttCCTCTCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACaaaaa      >  1:323179/1‑140 (MQ=255)                                                                                                                                 ctctCCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAaacaa   <  1:832793/140‑1 (MQ=255)                                                                                                                                  tctcCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAaacaac  >  2:580368/1‑140 (MQ=255)                                                                                                                                    tcCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAaacaa   >  1:151067/1‑137 (MQ=255)                                                                                                                                    tcCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAaacaa   <  2:1014437/137‑1 (MQ=255)                                                                                                                                    tcCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAaacaa   <  2:151067/137‑1 (MQ=255)                                                                                                                                    tcCCGCGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAaacaa   >  1:1014437/1‑137 (MQ=255)                                                                                                                                        |                                                                                                                                      TAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAAC  >  NC_000913/312926‑313194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑